Serine/threonine-protein kinase SSN3 - Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase SSN3
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cyclin-dependent kinase 8

Gene names

Name:	SSN3
Synonyms:	CDK8
Ordered Locus Names:	CAGL0L12650g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces ›

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the SRB8-11 complex, a regulatory module of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 567

567

Serine/threonine-protein kinase SSN3

PRO_0000312939

Regions

Domain

68 – 470

403

Protein kinase

Nucleotide binding

74 – 82

9

ATP By similarity

Compositional bias

538 – 546

9

Poly-Ala

Sites

Active site

293

1

Proton acceptor By similarity

Binding site

190

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6FKC6 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 4CED3A8B663AC2EF
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FASTA

567

63,532

        10         20         30         40         50         60 
MQNSGRDLWQ NQSGYMSSLQ QGLNKSNMVG ASTSHGKTPM LMANNDVFTI APYRTRKDNA 

        70         80         90        100        110        120 
RVSVLDKYEI IGYIAAGTYG KVYKAKSRQS SKSSSSTGSD SLAQDTKPTT EFSNTSSLQN 

       130        140        150        160        170        180 
AGTYGDMMGA HGPNSNNISA GGNTNPELST RNNPNNPRVP TSTIISGDKR NSENTDNRRK 

       190        200        210        220        230        240 
AETTMYYAIK KFKTEKDGIE QLHYTGISQS ACREMALCRE LDNNHLTKLV EIFLQKKSIY 

       250        260        270        280        290        300 
MVYEFAEHDL LQIIHFHSHP EKRMIPPRMI RSIMWQILDG VSYLHQNWVL HRDLKPANIM 

       310        320        330        340        350        360 
VTMDGVVKIG DLGLARKFSN MLQTMYTGDK VVVTIWYRAP ELLLGARHYT PAIDLWAVGC 

       370        380        390        400        410        420 
IFAELIGLQP IFKGEEAKMD SKKTVPFQAN QLQRILKILG TPTPKSWPHL QKYPEYEQLS 

       430        440        450        460        470        480 
KFPKYRDNLP GWFHSAGGRD KHALSLLYHL LNYNPIERID AINALDHSYF THGDMPVCEN 

       490        500        510        520        530        540 
VFEGLNYKYP ARRIHTNDND ILNLGLHKPK VPAKVVQPTM NNSTATLGGL GVNKRILAAA 

       550        560 
AAAAAAVSGN SSSQSSRNME PMKKKRK

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References

[1]

"Genome evolution in yeasts."
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.

Souciet J.-L.
Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380958 Genomic DNA. Translation: CAG62292.1.
RefSeq	XP_449318.1. XM_449318.1.
3D structure databases
ProteinModelPortal	Q6FKC6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2890604.
KEGG	cgr:CAGL0L12650g.
Phylogenomic databases
KO	K02208.
OMA	FHSHPEK.
OrthoDB	EOG470XRW.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSN3_CANGA

Accession

Primary (citable) accession number: Q6FKC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2004
Last modified:	May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families