Q6FIV3 (ERG27_CANGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-keto-steroid reductase
EC=1.1.1.270

Gene names

Name:	ERG27
Ordered Locus Names:	CAGL0M11506g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the reduction of the keto group on the C-3 of sterols By similarity.
Catalytic activity	4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NADP+ = 4-alpha-methyl-5-alpha-cholest-7-en-3-one + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-keto sterol reductase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	3-keto-steroid reductase		PRO_0000054591
Sites
	Active site	203	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FIV3 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 5636A881CD1F7645	FASTA	348	39,505
	10 20 30 40 50 60 MTSKTRKVAV ITGANSNLGL NIAYRLIERQ SADVRLTLVV TSRTLPRVRE VVELIKKFVA 70 80 90 100 110 120 TQEDPCSVDF DYLLVDFTNM VSVLNAYYDL NQKYESINYF FVNAAQGVYD GIDWIGAVKQ 130 140 150 160 170 180 VLSDPLEAVT NPTYRKQLVG VKSKDEMGLV FQANVFGPYY LIQKILPQLS KGKATVVWIS 190 200 210 220 230 240 SIMADPKHLS LQDIEMIKSD VTYEGSKRVV DLLHLATYKQ MKSQGIHQYV VQPGIFTSYS 250 260 270 280 290 300 FAKYLNFFTT FGMLFLFYLA RLLGSKWHNI DGYKAANAPV YVATLINPHF EHQEVKYGSA 310 320 330 340 SSRDGMEYIE TTDIDKTGSS DVLAYIEKKK LEWDDKLKDQ ITNSRIPI

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380959 Genomic DNA. Translation: CAG62821.1.
RefSeq	XP_449841.1. XM_449841.1.
3D structure databases
ProteinModelPortal	Q6FIV3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2891410.
GenomeReviews	Gene locus CAGL0M11506g in contig CR380959_GR.
KEGG	cgr:CAGL0M11506g.
Phylogenomic databases
eggNOG	fuNOG05767.
HOGENOM	HBG398311.
OMA	FLFYLAR.
OrthoDB	EOG4FBN2N.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K09827.
ProtoNet	Search...

Entry information

Entry name

ERG27_CANGA

Accession

Primary (citable) accession number: Q6FIV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	July 19, 2004
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program