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Protein

Pyruvate kinase 1

Gene

PYK1

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501SubstrateBy similarity
Metal bindingi52 – 521PotassiumBy similarity
Metal bindingi54 – 541PotassiumBy similarity
Metal bindingi85 – 851PotassiumBy similarity
Metal bindingi86 – 861Potassium; via carbonyl oxygenBy similarity
Sitei241 – 2411Transition state stabilizerBy similarity
Metal bindingi243 – 2431MagnesiumSequence Analysis
Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
Metal bindingi267 – 2671MagnesiumBy similarity
Binding sitei267 – 2671Substrate; via amide nitrogenBy similarity
Binding sitei299 – 2991SubstrateBy similarity
Binding sitei338 – 3381ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 1 (EC:2.7.1.40)
Short name:
PK 1
Gene namesi
Name:PYK1
Ordered Locus Names:CAGL0M12034g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
ProteomesiUP000002428: Chromosome M

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: CGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Pyruvate kinase 1PRO_0000112110Add
BLAST

Proteomic databases

PRIDEiQ6FIS9.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6FIS9.
SMRiQ6FIS9. Positions 3-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000021559.
InParanoidiQ6FIS9.
KOiK00873.
OMAiGMDIGKA.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6FIS9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSRLARLTT LQTSAGEELR RTSIIGTIGP KTNNPETLVA LRKAGLNIVR
60 70 80 90 100
MNFSHGSYEY HKSVIDNARK SEELYPGRPL AIALDTKGPE IRTGTTTNEV
110 120 130 140 150
DYPIPPNHEM IFTTDDKYAK ACDDKIMYLD YKNITKVISA GRIIYVDDGV
160 170 180 190 200
LSFEVLQVVD DKTLKVKSLN AGKICSHKGV NLPGTDVDLP ALSEKDKADL
210 220 230 240 250
RFGVENGVHM IFASFIRTAQ DVLTIREVLG EDGKDIKIVV KIENQQGVNN
260 270 280 290 300
FDEILKVTDA VMVARGDLGI EIPAPEVLAV QKKLIAKSNL AGKPVICATQ
310 320 330 340 350
MLESMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGN YPINAVTTMA
360 370 380 390 400
ETALIAEQAI AYLPNYDDIR NCTPKPTSTT ETVAASAVAA VFEQKAKAII
410 420 430 440 450
VLSTSGTTAR LVSKYRPNCP IILVTRNART ARFSHLYRGV FPFVYEKESV
460 470 480 490 500
SDWTEDVEAR LNFGIEQAIE FGILKKGDTY VSIQGFKAGV GHSNTLQVST

A
Length:501
Mass (Da):54,668
Last modified:July 19, 2004 - v1
Checksum:iD3F800A625D7FD4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380959 Genomic DNA. Translation: CAG62845.1.
RefSeqiXP_449865.1. XM_449865.1.

Genome annotation databases

GeneIDi2891415.
KEGGicgr:CAGL0M12034g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380959 Genomic DNA. Translation: CAG62845.1.
RefSeqiXP_449865.1. XM_449865.1.

3D structure databases

ProteinModelPortaliQ6FIS9.
SMRiQ6FIS9. Positions 3-500.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6FIS9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2891415.
KEGGicgr:CAGL0M12034g.

Phylogenomic databases

HOGENOMiHOG000021559.
InParanoidiQ6FIS9.
KOiK00873.
OMAiGMDIGKA.
OrthoDBiEOG7M6DJC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK1_CANGA
AccessioniPrimary (citable) accession number: Q6FIS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.