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Q6FIS9 (KPYK1_CANGA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase 1

Short name=PK 1
EC=2.7.1.40
Gene names
Name:PYK1
Ordered Locus Names:CAGL0M12034g
OrganismCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]
Taxonomic identifier284593 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
Pyruvate
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from direct assay PubMed 21085892. Source: CGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: InterPro

pyruvate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Pyruvate kinase 1
PRO_0000112110

Sites

Metal binding521Potassium By similarity
Metal binding541Potassium By similarity
Metal binding851Potassium By similarity
Metal binding861Potassium; via carbonyl oxygen By similarity
Metal binding2431Magnesium Potential
Metal binding2671Magnesium By similarity
Binding site501Substrate By similarity
Binding site2661Substrate; via amide nitrogen By similarity
Binding site2671Substrate; via amide nitrogen By similarity
Binding site2991Substrate By similarity
Binding site3381ADP Potential
Site2411Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6FIS9 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: D3F800A625D7FD4E

FASTA50154,668
        10         20         30         40         50         60 
MDSRLARLTT LQTSAGEELR RTSIIGTIGP KTNNPETLVA LRKAGLNIVR MNFSHGSYEY 

        70         80         90        100        110        120 
HKSVIDNARK SEELYPGRPL AIALDTKGPE IRTGTTTNEV DYPIPPNHEM IFTTDDKYAK 

       130        140        150        160        170        180 
ACDDKIMYLD YKNITKVISA GRIIYVDDGV LSFEVLQVVD DKTLKVKSLN AGKICSHKGV 

       190        200        210        220        230        240 
NLPGTDVDLP ALSEKDKADL RFGVENGVHM IFASFIRTAQ DVLTIREVLG EDGKDIKIVV 

       250        260        270        280        290        300 
KIENQQGVNN FDEILKVTDA VMVARGDLGI EIPAPEVLAV QKKLIAKSNL AGKPVICATQ 

       310        320        330        340        350        360 
MLESMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGN YPINAVTTMA ETALIAEQAI 

       370        380        390        400        410        420 
AYLPNYDDIR NCTPKPTSTT ETVAASAVAA VFEQKAKAII VLSTSGTTAR LVSKYRPNCP 

       430        440        450        460        470        480 
IILVTRNART ARFSHLYRGV FPFVYEKESV SDWTEDVEAR LNFGIEQAIE FGILKKGDTY 

       490        500 
VSIQGFKAGV GHSNTLQVST A 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR380959 Genomic DNA. Translation: CAG62845.1.
RefSeqXP_449865.1. XM_449865.1.

3D structure databases

ProteinModelPortalQ6FIS9.
SMRQ6FIS9. Positions 3-500.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ6FIS9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2891415.
KEGGcgr:CAGL0M12034g.

Phylogenomic databases

HOGENOMHOG000021559.
KOK00873.
OMALIVESCE.
OrthoDBEOG7M6DJC.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK1_CANGA
AccessionPrimary (citable) accession number: Q6FIS9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: November 13, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways