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Protein

Pyruvate kinase 1

Gene

PYK1

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (GPD1), Glyceraldehyde-3-phosphate dehydrogenase 2 (GPD2)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase (GPM1), Phosphoglycerate mutase (CAGL0K01705g)
  4. Enolase 1 (ENO1), Enolase 2 (ENO2)
  5. Pyruvate kinase 2 (PYK2), Pyruvate kinase 1 (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Metal bindingi52PotassiumBy similarity1
Metal bindingi54PotassiumBy similarity1
Metal bindingi85PotassiumBy similarity1
Metal bindingi86Potassium; via carbonyl oxygenBy similarity1
Sitei241Transition state stabilizerBy similarity1
Metal bindingi243MagnesiumSequence analysis1
Binding sitei266Substrate; via amide nitrogenBy similarity1
Metal bindingi267MagnesiumBy similarity1
Binding sitei267Substrate; via amide nitrogenBy similarity1
Binding sitei299SubstrateBy similarity1
Binding sitei338ADPSequence analysis1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 1 (EC:2.7.1.40)
Short name:
PK 1
Gene namesi
Name:PYK1
Ordered Locus Names:CAGL0M12034g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
Proteomesi
  • UP000002428 Componenti: Chromosome M

Organism-specific databases

CGDiCAL0137479. PYK1.
EuPathDBiFungiDB:CAGL0M12034g.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121101 – 501Pyruvate kinase 1Add BLAST501

Proteomic databases

PRIDEiQ6FIS9.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi284593.XP_449865.1.

Structurei

3D structure databases

ProteinModelPortaliQ6FIS9.
SMRiQ6FIS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOGENOMiHOG000021559.
InParanoidiQ6FIS9.
KOiK00873.
OMAiKHEAIEQ.
OrthoDBiEOG092C1RXZ.

Family and domain databases

CDDicd00288. Pyruvate_Kinase. 1 hit.
Gene3Di3.40.1380.20. 1 hit.
InterProiView protein in InterPro
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR036918. Pyrv_Knase_C_sf.
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiView protein in Pfam
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiView protein in PROSITE
PS00110. PYRUVATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6FIS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRLARLTT LQTSAGEELR RTSIIGTIGP KTNNPETLVA LRKAGLNIVR
60 70 80 90 100
MNFSHGSYEY HKSVIDNARK SEELYPGRPL AIALDTKGPE IRTGTTTNEV
110 120 130 140 150
DYPIPPNHEM IFTTDDKYAK ACDDKIMYLD YKNITKVISA GRIIYVDDGV
160 170 180 190 200
LSFEVLQVVD DKTLKVKSLN AGKICSHKGV NLPGTDVDLP ALSEKDKADL
210 220 230 240 250
RFGVENGVHM IFASFIRTAQ DVLTIREVLG EDGKDIKIVV KIENQQGVNN
260 270 280 290 300
FDEILKVTDA VMVARGDLGI EIPAPEVLAV QKKLIAKSNL AGKPVICATQ
310 320 330 340 350
MLESMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGN YPINAVTTMA
360 370 380 390 400
ETALIAEQAI AYLPNYDDIR NCTPKPTSTT ETVAASAVAA VFEQKAKAII
410 420 430 440 450
VLSTSGTTAR LVSKYRPNCP IILVTRNART ARFSHLYRGV FPFVYEKESV
460 470 480 490 500
SDWTEDVEAR LNFGIEQAIE FGILKKGDTY VSIQGFKAGV GHSNTLQVST

A
Length:501
Mass (Da):54,668
Last modified:July 19, 2004 - v1
Checksum:iD3F800A625D7FD4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380959 Genomic DNA. Translation: CAG62845.1.
RefSeqiXP_449865.1. XM_449865.1.

Genome annotation databases

EnsemblFungiiCAG62845; CAG62845; CAGL0M12034g.
GeneIDi2891415.
KEGGicgr:CAGL0M12034g.

Similar proteinsi

Entry informationi

Entry nameiKPYK1_CANGA
AccessioniPrimary (citable) accession number: Q6FIS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: October 25, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families