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Protein

AN1-type zinc finger protein 6

Gene

ZFAND6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'-linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1. Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 4235A20-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 18944AN1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cellular response to tumor necrosis factor Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • protein targeting to peroxisome Source: UniProtKB
  • regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
AN1-type zinc finger protein 6
Alternative name(s):
Associated with PRK1 protein
Zinc finger A20 domain-containing protein 3
Gene namesi
Name:ZFAND6
Synonyms:AWP1, ZA20D3
ORF Names:HT032
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:30164. ZFAND6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134883256.

Polymorphism and mutation databases

BioMutaiZFAND6.
DMDMi110288083.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208AN1-type zinc finger protein 6PRO_0000245235Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei204 – 2041N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ6FIF0.
MaxQBiQ6FIF0.
PaxDbiQ6FIF0.
PeptideAtlasiQ6FIF0.
PRIDEiQ6FIF0.
TopDownProteomicsiQ6FIF0-1. [Q6FIF0-1]

PTM databases

iPTMnetiQ6FIF0.

Expressioni

Tissue specificityi

Widely expressed with high level in heart, skeletal muscle, liver, kidney and placenta.1 Publication

Gene expression databases

BgeeiQ6FIF0.
CleanExiHS_ZFAND6.
ExpressionAtlasiQ6FIF0. baseline and differential.
GenevisibleiQ6FIF0. HS.

Organism-specific databases

HPAiHPA034845.

Interactioni

Subunit structurei

Interacts with PKN1 (By similarity). Interacts with TRAF2. Interacts with mono- and polyubiquitin. Interacts with PEX6. Interacts with PEX5 (Cys-linked ubiquitinated) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TRAF2Q129336EBI-724630,EBI-355744
TRAF5O004633EBI-724630,EBI-523498

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119975. 14 interactions.
IntActiQ6FIF0. 3 interactions.
MINTiMINT-1405113.
STRINGi9606.ENSP00000261749.

Structurei

3D structure databases

ProteinModelPortaliQ6FIF0.
SMRiQ6FIF0. Positions 11-44, 135-190.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 10967Ser-richAdd
BLAST

Domaini

The A20-type zinc finger domain mediates regulation of NF-kappa-B activity.
The AN1-type zinc finger domain mediates association with TRAF2.

Sequence similaritiesi

Contains 1 A20-type zinc finger.PROSITE-ProRule annotation
Contains 1 AN1-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri8 – 4235A20-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri146 – 18944AN1-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3173. Eukaryota.
ENOG4111UWC. LUCA.
GeneTreeiENSGT00510000046525.
HOGENOMiHOG000238229.
HOVERGENiHBG053349.
InParanoidiQ6FIF0.
OMAiNVPEAQS.
OrthoDBiEOG7TTQ9W.
PhylomeDBiQ6FIF0.
TreeFamiTF313612.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR002653. Znf_A20.
IPR000058. Znf_AN1.
[Graphical view]
PfamiPF01754. zf-A20. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00259. ZnF_A20. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51036. ZF_A20. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6FIF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQETNHSQV PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QNSSNGRISP
60 70 80 90 100
PATSVSSLSE SLPVQCTDGS VPEAQSALDS TSSSMQPSPV SNQSLLSESV
110 120 130 140 150
ASSQLDSTSV DKAVPETEDV QASVSDTAQQ PSEEQSKSLE KPKQKKNRCF
160 170 180 190 200
MCRKKVGLTG FECRCGNVYC GVHRYSDVHN CSYNYKADAA EKIRKENPVV

VGEKIQKI
Length:208
Mass (Da):22,555
Last modified:July 11, 2006 - v2
Checksum:i28B443F2E6D84493
GO
Isoform 2 (identifier: Q6FIF0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-63: Missing.

Note: No experimental confirmation available.
Show »
Length:196
Mass (Da):21,396
Checksum:i030B4F52C8324630
GO

Sequence cautioni

The sequence AAD17528.1 differs from that shown. Reason: Frameshift at position 141. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti138 – 1381S → P in CAB66533 (Ref. 3) Curated
Sequence conflicti138 – 1381S → P in CAG38507 (Ref. 5) Curated
Sequence conflicti140 – 1401E → D in AAD17528 (Ref. 2) Curated
Sequence conflicti179 – 1791H → L in CAB66533 (Ref. 3) Curated
Sequence conflicti179 – 1791H → L in CAG38507 (Ref. 5) Curated
Sequence conflicti197 – 1971N → D in CAG38507 (Ref. 5) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei52 – 6312Missing in isoform 2. 1 PublicationVSP_019652Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251095 mRNA. Translation: CAC14876.1.
AF061739 mRNA. Translation: AAD17528.1. Frameshift.
AF261138 mRNA. Translation: AAG44674.1.
AL136598 mRNA. Translation: CAB66533.1.
CR533476 mRNA. Translation: CAG38507.1.
CH471136 Genomic DNA. Translation: EAW99122.1.
CH471136 Genomic DNA. Translation: EAW99123.1.
CH471136 Genomic DNA. Translation: EAW99124.1.
CH471136 Genomic DNA. Translation: EAW99125.1.
CH471136 Genomic DNA. Translation: EAW99127.1.
BC005283 mRNA. Translation: AAH05283.1.
CCDSiCCDS10313.1. [Q6FIF0-1]
CCDS58395.1. [Q6FIF0-2]
RefSeqiNP_001229840.1. NM_001242911.1. [Q6FIF0-1]
NP_001229841.1. NM_001242912.1. [Q6FIF0-1]
NP_001229842.1. NM_001242913.1. [Q6FIF0-1]
NP_001229843.1. NM_001242914.1. [Q6FIF0-1]
NP_001229844.1. NM_001242915.1. [Q6FIF0-1]
NP_001229845.1. NM_001242916.1. [Q6FIF0-1]
NP_001229846.1. NM_001242917.1. [Q6FIF0-2]
NP_061879.2. NM_019006.3. [Q6FIF0-1]
XP_011519986.1. XM_011521684.1. [Q6FIF0-1]
UniGeneiHs.596679.
Hs.744030.

Genome annotation databases

EnsembliENST00000261749; ENSP00000261749; ENSG00000086666. [Q6FIF0-1]
ENST00000558087; ENSP00000453888; ENSG00000086666. [Q6FIF0-1]
ENST00000558494; ENSP00000454137; ENSG00000086666. [Q6FIF0-1]
ENST00000559157; ENSP00000454152; ENSG00000086666. [Q6FIF0-2]
ENST00000559775; ENSP00000453709; ENSG00000086666. [Q6FIF0-1]
ENST00000559835; ENSP00000453291; ENSG00000086666. [Q6FIF0-1]
ENST00000561060; ENSP00000452735; ENSG00000086666. [Q6FIF0-1]
ENST00000613266; ENSP00000479071; ENSG00000086666. [Q6FIF0-1]
ENST00000616533; ENSP00000478129; ENSG00000086666. [Q6FIF0-1]
ENST00000618205; ENSP00000484971; ENSG00000086666. [Q6FIF0-1]
GeneIDi54469.
KEGGihsa:54469.
UCSCiuc002bfe.2. human. [Q6FIF0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251095 mRNA. Translation: CAC14876.1.
AF061739 mRNA. Translation: AAD17528.1. Frameshift.
AF261138 mRNA. Translation: AAG44674.1.
AL136598 mRNA. Translation: CAB66533.1.
CR533476 mRNA. Translation: CAG38507.1.
CH471136 Genomic DNA. Translation: EAW99122.1.
CH471136 Genomic DNA. Translation: EAW99123.1.
CH471136 Genomic DNA. Translation: EAW99124.1.
CH471136 Genomic DNA. Translation: EAW99125.1.
CH471136 Genomic DNA. Translation: EAW99127.1.
BC005283 mRNA. Translation: AAH05283.1.
CCDSiCCDS10313.1. [Q6FIF0-1]
CCDS58395.1. [Q6FIF0-2]
RefSeqiNP_001229840.1. NM_001242911.1. [Q6FIF0-1]
NP_001229841.1. NM_001242912.1. [Q6FIF0-1]
NP_001229842.1. NM_001242913.1. [Q6FIF0-1]
NP_001229843.1. NM_001242914.1. [Q6FIF0-1]
NP_001229844.1. NM_001242915.1. [Q6FIF0-1]
NP_001229845.1. NM_001242916.1. [Q6FIF0-1]
NP_001229846.1. NM_001242917.1. [Q6FIF0-2]
NP_061879.2. NM_019006.3. [Q6FIF0-1]
XP_011519986.1. XM_011521684.1. [Q6FIF0-1]
UniGeneiHs.596679.
Hs.744030.

3D structure databases

ProteinModelPortaliQ6FIF0.
SMRiQ6FIF0. Positions 11-44, 135-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119975. 14 interactions.
IntActiQ6FIF0. 3 interactions.
MINTiMINT-1405113.
STRINGi9606.ENSP00000261749.

PTM databases

iPTMnetiQ6FIF0.

Polymorphism and mutation databases

BioMutaiZFAND6.
DMDMi110288083.

Proteomic databases

EPDiQ6FIF0.
MaxQBiQ6FIF0.
PaxDbiQ6FIF0.
PeptideAtlasiQ6FIF0.
PRIDEiQ6FIF0.
TopDownProteomicsiQ6FIF0-1. [Q6FIF0-1]

Protocols and materials databases

DNASUi54469.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261749; ENSP00000261749; ENSG00000086666. [Q6FIF0-1]
ENST00000558087; ENSP00000453888; ENSG00000086666. [Q6FIF0-1]
ENST00000558494; ENSP00000454137; ENSG00000086666. [Q6FIF0-1]
ENST00000559157; ENSP00000454152; ENSG00000086666. [Q6FIF0-2]
ENST00000559775; ENSP00000453709; ENSG00000086666. [Q6FIF0-1]
ENST00000559835; ENSP00000453291; ENSG00000086666. [Q6FIF0-1]
ENST00000561060; ENSP00000452735; ENSG00000086666. [Q6FIF0-1]
ENST00000613266; ENSP00000479071; ENSG00000086666. [Q6FIF0-1]
ENST00000616533; ENSP00000478129; ENSG00000086666. [Q6FIF0-1]
ENST00000618205; ENSP00000484971; ENSG00000086666. [Q6FIF0-1]
GeneIDi54469.
KEGGihsa:54469.
UCSCiuc002bfe.2. human. [Q6FIF0-1]

Organism-specific databases

CTDi54469.
GeneCardsiZFAND6.
HGNCiHGNC:30164. ZFAND6.
HPAiHPA034845.
MIMi610183. gene.
neXtProtiNX_Q6FIF0.
PharmGKBiPA134883256.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3173. Eukaryota.
ENOG4111UWC. LUCA.
GeneTreeiENSGT00510000046525.
HOGENOMiHOG000238229.
HOVERGENiHBG053349.
InParanoidiQ6FIF0.
OMAiNVPEAQS.
OrthoDBiEOG7TTQ9W.
PhylomeDBiQ6FIF0.
TreeFamiTF313612.

Miscellaneous databases

ChiTaRSiZFAND6. human.
GenomeRNAii54469.
PROiQ6FIF0.
SOURCEiSearch...

Gene expression databases

BgeeiQ6FIF0.
CleanExiHS_ZFAND6.
ExpressionAtlasiQ6FIF0. baseline and differential.
GenevisibleiQ6FIF0. HS.

Family and domain databases

Gene3Di4.10.1110.10. 1 hit.
InterProiIPR002653. Znf_A20.
IPR000058. Znf_AN1.
[Graphical view]
PfamiPF01754. zf-A20. 1 hit.
PF01428. zf-AN1. 1 hit.
[Graphical view]
SMARTiSM00259. ZnF_A20. 1 hit.
SM00154. ZnF_AN1. 1 hit.
[Graphical view]
PROSITEiPS51036. ZF_A20. 1 hit.
PS51039. ZF_AN1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo."
    Duan W., Sun B., Li T.W., Tan B.J., Lee M.K., Teo T.S.
    Gene 256:113-121(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Uterus.
  2. "A novel human gene expressed in acute promyelocytic leukemia cell line NB4."
    Wu J., Liu T., Zhang J., Mao M., Zhang Q., Fu G., Shen Y., Zhou J., Yu Y., Wang Z., Chen S., Chen Z.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Hypothalamus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Identification of polyubiquitin binding proteins involved in NF-kappaB signaling using protein arrays."
    Fenner B.J., Scannell M., Prehn J.H.
    Biochim. Biophys. Acta 1794:1010-1016(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POLYUBIQUITIN.
  10. "AWP1 binds to tumor necrosis factor receptor-associated factor 2 (TRAF2) and is involved in TRAF2-mediated nuclear factor-kappaB signaling."
    Chang E.J., Ha J., Kang S.S., Lee Z.H., Kim H.H.
    Int. J. Biochem. Cell Biol. 43:1612-1620(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRAF2.
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiZFAN6_HUMAN
AccessioniPrimary (citable) accession number: Q6FIF0
Secondary accession number(s): D3DW92
, D3DW94, O95792, Q9BQF7, Q9GZY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: July 6, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.