Q6FI81 (CPIN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anamorsin

Alternative name(s):
Cytokine-induced apoptosis inhibitor 1
Fe-S cluster assembly protein DRE2 homolog

Gene names

Name:	CIAPIN1
ORF Names:	CUA001, PRO0915

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be required for the maturation of extramitochondrial Fe/S proteins By similarity. Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells By similarity. HAMAP-Rule MF_03115
Subcellular location	Cytoplasm. Nucleus Ref.10.
Tissue specificity	Ubiquitously expressed. Highly expressed in heart, liver and pancreas. Ref.9
Miscellaneous	'Ana-mors-in' means 'anti-death molecule' in Latin. HAMAP-Rule MF_03115
Sequence similarities	Belongs to the anamorsin family.
Sequence caution	The sequence AAC24312.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW hemopoiesis Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from sequence or structural similarity Ref.9. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.10. Source: UniProtKB nucleolus Inferred from direct assay Ref.10. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6FI81-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6FI81-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6FI81-3)

The sequence of this isoform differs from the canonical sequence as follows:
     53-66: SAHKESSFDIILSG → C

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 312

312

Anamorsin HAMAP-Rule MF_03115

PRO_0000079288

Amino acid modifications

Modified residue

182

1

Phosphoserine Ref.13

Modified residue

183

1

Phosphoserine Ref.13

Modified residue

305

1

Phosphoserine Ref.14 Ref.16

Modified residue

307

1

Phosphoserine Ref.12 Ref.14

Natural variations

Alternative sequence

1 – 204

204

Missing in isoform 2.

VSP_012360

Alternative sequence

53 – 66

14

SAHKE…IILSG → C in isoform 3.

VSP_012361

Natural variant

34

1

A → E.
Corresponds to variant rs11557672 [ dbSNP | Ensembl ].

VAR_033747

Natural variant

52

1

Q → E.
Corresponds to variant rs11557674 [ dbSNP | Ensembl ].

VAR_033748

Experimental info

Sequence conflict

142

1

S → F in CAB66548. Ref.2

Sequence conflict

142

1

S → F in CAG38576. Ref.4

Sequence conflict

278

1

Y → C in CAG38576. Ref.4

Sequence conflict

289

1

P → T in CAB66548. Ref.2

Sequence conflict

289

1

P → T in CAG38576. Ref.4

Sequence conflict

310

1

H → N in CAB66548. Ref.2

Sequence conflict

310

1

H → N in CAG38576. Ref.4

Secondary structure

1

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312

Helix Strand Turn

Details...

Turn

2 – 5

4

Beta strand

10 – 16

7

Helix

22 – 35

14

Turn

36 – 39

4

Beta strand

40 – 46

7

Helix

47 – 53

7

Beta strand

60 – 65

6

Beta strand

68 – 70

3

Helix

77 – 86

10

Beta strand

87 – 103

17

Beta strand

105 – 109

5

Helix

112 – 121

10

Beta strand

125 – 133

9

Helix

137 – 146

10

Beta strand

152 – 162

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 70A71D44074ABFBA	FASTA	312	33,582
	10 20 30 40 50 60 MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL LQSAHKESSF 70 80 90 100 110 120 DIILSGLVPG STTLHSAEIL AEIARILRPG GCLFLKEPVE TAVDNNSKVK TASKLCSALT 130 140 150 160 170 180 LSGLVEVKEL QREPLTPEEV QSVREHLGHE SDNLLFVQIT GKKPNFEVGS SRQLKLSITK 190 200 210 220 230 240 KSSPSVKPAV DPAAAKLWTL SANDMEDDSM DLIDSDELLD PEDLKKPDPA SLRAASCGEG 250 260 270 280 290 300 KKRKACKNCT CGLAEELEKE KSREQMSSQP KSACGNCYLG DAFRCASCPY LGMPAFKPGE 310 KVLLSDSNLH DA
	Isoform 2 [UniParc]. Checksum: C9B53F80FA24398A	FASTA	108	11,735
	10 20 30 40 50 60 MEDDSMDLID SDELLDPEDL KKPDPASLRA ASCGEGKKRK ACKNCTCGLA EELEKEKSRE 70 80 90 100 QMSSQPKSAC GNCYLGDAFR CASCPYLGMP AFKPGEKVLL SDSNLHDA
	Isoform 3 [UniParc]. Checksum: 29CA61B57C265FE7	FASTA	299	32,213
	10 20 30 40 50 60 MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL LQCLVPGSTT 70 80 90 100 110 120 LHSAEILAEI ARILRPGGCL FLKEPVETAV DNNSKVKTAS KLCSALTLSG LVEVKELQRE 130 140 150 160 170 180 PLTPEEVQSV REHLGHESDN LLFVQITGKK PNFEVGSSRQ LKLSITKKSS PSVKPAVDPA 190 200 210 220 230 240 AAKLWTLSAN DMEDDSMDLI DSDELLDPED LKKPDPASLR AASCGEGKKR KACKNCTCGL 250 260 270 280 290 AEELEKEKSR EQMSSQPKSA CGNCYLGDAF RCASCPYLGM PAFKPGEKVL LSDSNLHDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Yang Y., Xu X., Gao G., Xiao H., Chen Z., Han Z. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Adrenal tumor.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[3]	"Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs." Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C., Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C., He F. Genome Res. 11:1392-1403(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Fetal liver.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[6]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain, Placenta, Skin and Testis.
[9]	"Identification of a cytokine-induced antiapoptotic molecule anamorsin essential for definitive hematopoiesis." Shibayama H., Takai E., Matsumura I., Kouno M., Morii E., Kitamura Y., Takeda J., Kanakura Y. J. Exp. Med. 199:581-592(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"Subcellular localization of CIAPIN1." Hao Z., Li X., Qiao T., Du R., Zhang G., Fan D. J. Histochem. Cytochem. 54:1437-1444(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[11]	"Successful expression and purification of human CIAPIN1 in baculovirus-insect cell system and application of this system to investigation of its potential methyltransferase activity." Hao Z., Li X., Qiao T., Fan D. Int. J. Biol. Macromol. 42:27-32(2008) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF METHYLTRANSFERASE ACTIVITY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182 AND SER-183, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305 AND SER-307, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305, MASS SPECTROMETRY.
[17]	"Solution structure of the N-terminal domain in human cytokine-induced apoptosis inhibitor anamorsin." RIKEN structural genomics initiative (RSGI) Submitted (APR-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-171.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF248964 mRNA. Translation: AAG44562.1.
AL136613 mRNA. Translation: CAB66548.1.
AF116609 mRNA. Translation: AAF71034.1.
CR533545 mRNA. Translation: CAG38576.1.
AK292281 mRNA. Translation: BAF84970.1.
AC004382 Genomic DNA. Translation: AAC24311.1.
AC004382 Genomic DNA. Translation: AAC24312.1. Sequence problems.
CH471092 Genomic DNA. Translation: EAW82923.1.
BC002568 mRNA. Translation: AAH02568.1.
BC024196 mRNA. Translation: AAH24196.1.
BC067303 mRNA. Translation: AAH67303.1.
BC071740 mRNA. Translation: AAH71740.1.

IPI

IPI00025333.
IPI00387130.
IPI00513770.

RefSeq

NP_064709.2. NM_020313.2.

UniGene

Hs.4900.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LD4	NMR	-	A	1-172	[»]
2YUI	NMR	-	A	1-170	[»]

ProteinModelPortal

Q6FI81.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q6FI81. 3 interactions.

MINT

MINT-1469842.

STRING

9606.ENSP00000377914.

PTM databases

PhosphoSite

Q6FI81.

Polymorphism databases

DMDM

57012667.

Proteomic databases

PaxDb

Q6FI81.

PRIDE

Q6FI81.

Protocols and materials databases

DNASU

57019.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000394391; ENSP00000377914; ENSG00000005194.
ENST00000567518; ENSP00000456114; ENSG00000005194.

GeneID

57019.

KEGG

hsa:57019.

UCSC

uc002ell.1. human.
uc002elm.1. human.

Organism-specific databases

CTD

57019.

GeneCards

GC16M057462.

HGNC

HGNC:28050. CIAPIN1.

HPA

HPA041350.
HPA042182.

MIM

608943. gene.

neXtProt

NX_Q6FI81.

PharmGKB

PA134978864.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5636.

HOGENOM

HOG000241118.

HOVERGEN

HBG051104.

OMA

QFVAVVW.

OrthoDB

EOG4NKBWN.

Gene expression databases

ArrayExpress

Q6FI81.

Bgee

Q6FI81.

CleanEx

HS_CIAPIN1.

Genevestigator

Q6FI81.

Family and domain databases

HAMAP

MF_03115. Anamorsin.

InterPro

IPR007785. CIAPIN1.
[Graphical view]

PANTHER

PTHR13273. PTHR13273. 1 hit.

Pfam

PF05093. CIAPIN1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CIAPIN1. human.

EvolutionaryTrace

Q6FI81.

GenomeRNAi

57019.

NextBio

62770.

SOURCE

Search...

Entry information

Entry name

CPIN1_HUMAN

Accession

Primary (citable) accession number: Q6FI81
Secondary accession number(s): A8K8B6 , O75206, O75207, Q9H0W1, Q9P1L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.