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Protein

Anamorsin

Gene

CIAPIN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells (By similarity). Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S cluster from NADPH via the FAD- and FMN-containing protein NDOR1.By similarity1 Publication

Cofactori

[2Fe-2S] clusterUniRule annotation2 PublicationsNote: Binds 1 [2Fe-2S] cluster per subunit.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi237Iron-sulfur (2Fe-2S)1
Metal bindingi246Iron-sulfur (2Fe-2S)1
Metal bindingi249Iron-sulfur (2Fe-2S)1
Metal bindingi251Iron-sulfur (2Fe-2S)1

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • hemopoiesis Source: UniProtKB-HAMAP
  • iron-sulfur cluster assembly Source: GO_Central
  • negative regulation of apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-2564830. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
AnamorsinUniRule annotation
Alternative name(s):
Cytokine-induced apoptosis inhibitor 1UniRule annotation
Fe-S cluster assembly protein DRE2 homologUniRule annotation
Gene namesi
Name:CIAPIN1UniRule annotation
ORF Names:CUA001, PRO0915
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:28050. CIAPIN1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • mitochondrial intermembrane space Source: UniProtKB-SubCell
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi57019.
OpenTargetsiENSG00000005194.
PharmGKBiPA134978864.

Polymorphism and mutation databases

BioMutaiCIAPIN1.
DMDMi57012667.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000792881 – 312AnamorsinAdd BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136PhosphothreonineCombined sources1
Modified residuei177PhosphoserineCombined sources1
Modified residuei182PhosphoserineCombined sources1
Modified residuei183PhosphoserineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Modified residuei272PhosphoserineBy similarity1
Modified residuei305PhosphoserineCombined sources1
Modified residuei307PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6FI81.
MaxQBiQ6FI81.
PaxDbiQ6FI81.
PeptideAtlasiQ6FI81.
PRIDEiQ6FI81.
TopDownProteomicsiQ6FI81-1. [Q6FI81-1]

PTM databases

iPTMnetiQ6FI81.
PhosphoSitePlusiQ6FI81.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in heart, liver and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000005194.
CleanExiHS_CIAPIN1.
ExpressionAtlasiQ6FI81. baseline and differential.
GenevisibleiQ6FI81. HS.

Organism-specific databases

HPAiHPA041350.
HPA042182.

Interactioni

Subunit structurei

Monomer. Interacts with NDOR1; its oxidized form can be reduced by NDOR1. Interacts with CHCHD4 and may be a substrate for CHCHD4 chaperone activity.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GLRX3O7600313EBI-750511,EBI-374781
MEOX2A4D1273EBI-750511,EBI-10172134
NDOR1Q9UHB45EBI-750511,EBI-10249760

Protein-protein interaction databases

BioGridi121328. 50 interactors.
DIPiDIP-61697N.
IntActiQ6FI81. 8 interactors.
MINTiMINT-1469842.
STRINGi9606.ENSP00000377914.

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Beta strandi11 – 16Combined sources6
Helixi22 – 36Combined sources15
Turni37 – 39Combined sources3
Beta strandi40 – 46Combined sources7
Helixi47 – 49Combined sources3
Helixi50 – 53Combined sources4
Beta strandi60 – 66Combined sources7
Beta strandi68 – 70Combined sources3
Helixi77 – 86Combined sources10
Beta strandi87 – 102Combined sources16
Beta strandi105 – 109Combined sources5
Helixi112 – 121Combined sources10
Beta strandi125 – 134Combined sources10
Helixi137 – 147Combined sources11
Beta strandi154 – 162Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LD4NMR-A1-172[»]
2YUINMR-A1-170[»]
4M7RX-ray1.80A/B1-172[»]
ProteinModelPortaliQ6FI81.
SMRiQ6FI81.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6FI81.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 172N-terminal domainAdd BLAST172
Regioni173 – 223LinkerAdd BLAST51
Regioni224 – 312Intrinsically disorderedAdd BLAST89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi274 – 277Cx2C motif 14
Motifi285 – 288Cx2C motif 24

Domaini

The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine.1 Publication
The C-terminal domain binds one 2Fe-2S iron-sulfur cluster but is otherwise mostly in an intrinsically disordered conformation.
The twin Cx2C motifs are involved in the recognition by the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.

Sequence similaritiesi

Belongs to the anamorsin family.UniRule annotation

Phylogenomic databases

eggNOGiKOG4020. Eukaryota.
COG5636. LUCA.
GeneTreeiENSGT00390000011417.
HOGENOMiHOG000241118.
HOVERGENiHBG051104.
InParanoidiQ6FI81.
OMAiMWTLSAN.
OrthoDBiEOG091G0OTI.
PhylomeDBiQ6FI81.
TreeFamiTF314449.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03115. Anamorsin. 1 hit.
InterProiIPR007785. Anamorsin.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13273. PTHR13273. 1 hit.
PfamiPF05093. CIAPIN1. 2 hits.
PF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6FI81-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL
60 70 80 90 100
LQSAHKESSF DIILSGLVPG STTLHSAEIL AEIARILRPG GCLFLKEPVE
110 120 130 140 150
TAVDNNSKVK TASKLCSALT LSGLVEVKEL QREPLTPEEV QSVREHLGHE
160 170 180 190 200
SDNLLFVQIT GKKPNFEVGS SRQLKLSITK KSSPSVKPAV DPAAAKLWTL
210 220 230 240 250
SANDMEDDSM DLIDSDELLD PEDLKKPDPA SLRAASCGEG KKRKACKNCT
260 270 280 290 300
CGLAEELEKE KSREQMSSQP KSACGNCYLG DAFRCASCPY LGMPAFKPGE
310
KVLLSDSNLH DA
Length:312
Mass (Da):33,582
Last modified:January 4, 2005 - v2
Checksum:i70A71D44074ABFBA
GO
Isoform 2 (identifier: Q6FI81-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Note: No experimental confirmation available.
Show »
Length:108
Mass (Da):11,735
Checksum:iC9B53F80FA24398A
GO
Isoform 3 (identifier: Q6FI81-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     53-66: SAHKESSFDIILSG → C

Show »
Length:299
Mass (Da):32,213
Checksum:i29CA61B57C265FE7
GO

Sequence cautioni

The sequence AAC24312 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142S → F in CAB66548 (PubMed:11230166).Curated1
Sequence conflicti142S → F in CAG38576 (Ref. 4) Curated1
Sequence conflicti278Y → C in CAG38576 (Ref. 4) Curated1
Sequence conflicti289P → T in CAB66548 (PubMed:11230166).Curated1
Sequence conflicti289P → T in CAG38576 (Ref. 4) Curated1
Sequence conflicti310H → N in CAB66548 (PubMed:11230166).Curated1
Sequence conflicti310H → N in CAG38576 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03374734A → E.Corresponds to variant rs11557672dbSNPEnsembl.1
Natural variantiVAR_03374852Q → E.Corresponds to variant rs11557674dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0123601 – 204Missing in isoform 2. 1 PublicationAdd BLAST204
Alternative sequenceiVSP_01236153 – 66SAHKE…IILSG → C in isoform 3. 3 PublicationsAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248964 mRNA. Translation: AAG44562.1.
AL136613 mRNA. Translation: CAB66548.1.
AF116609 mRNA. Translation: AAF71034.1.
CR533545 mRNA. Translation: CAG38576.1.
AK292281 mRNA. Translation: BAF84970.1.
AC004382 Genomic DNA. Translation: AAC24311.1.
AC004382 Genomic DNA. Translation: AAC24312.1. Sequence problems.
CH471092 Genomic DNA. Translation: EAW82923.1.
BC002568 mRNA. Translation: AAH02568.1.
BC024196 mRNA. Translation: AAH24196.1.
BC067303 mRNA. Translation: AAH67303.1.
BC071740 mRNA. Translation: AAH71740.1.
CCDSiCCDS10781.2. [Q6FI81-1]
CCDS76876.1. [Q6FI81-3]
RefSeqiNP_001295276.1. NM_001308347.1. [Q6FI81-3]
NP_001295287.1. NM_001308358.1.
NP_064709.2. NM_020313.3. [Q6FI81-1]
UniGeneiHs.4900.

Genome annotation databases

EnsembliENST00000394391; ENSP00000377914; ENSG00000005194. [Q6FI81-1]
ENST00000567518; ENSP00000456114; ENSG00000005194. [Q6FI81-3]
GeneIDi57019.
KEGGihsa:57019.
UCSCiuc002ell.2. human. [Q6FI81-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248964 mRNA. Translation: AAG44562.1.
AL136613 mRNA. Translation: CAB66548.1.
AF116609 mRNA. Translation: AAF71034.1.
CR533545 mRNA. Translation: CAG38576.1.
AK292281 mRNA. Translation: BAF84970.1.
AC004382 Genomic DNA. Translation: AAC24311.1.
AC004382 Genomic DNA. Translation: AAC24312.1. Sequence problems.
CH471092 Genomic DNA. Translation: EAW82923.1.
BC002568 mRNA. Translation: AAH02568.1.
BC024196 mRNA. Translation: AAH24196.1.
BC067303 mRNA. Translation: AAH67303.1.
BC071740 mRNA. Translation: AAH71740.1.
CCDSiCCDS10781.2. [Q6FI81-1]
CCDS76876.1. [Q6FI81-3]
RefSeqiNP_001295276.1. NM_001308347.1. [Q6FI81-3]
NP_001295287.1. NM_001308358.1.
NP_064709.2. NM_020313.3. [Q6FI81-1]
UniGeneiHs.4900.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LD4NMR-A1-172[»]
2YUINMR-A1-170[»]
4M7RX-ray1.80A/B1-172[»]
ProteinModelPortaliQ6FI81.
SMRiQ6FI81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121328. 50 interactors.
DIPiDIP-61697N.
IntActiQ6FI81. 8 interactors.
MINTiMINT-1469842.
STRINGi9606.ENSP00000377914.

PTM databases

iPTMnetiQ6FI81.
PhosphoSitePlusiQ6FI81.

Polymorphism and mutation databases

BioMutaiCIAPIN1.
DMDMi57012667.

Proteomic databases

EPDiQ6FI81.
MaxQBiQ6FI81.
PaxDbiQ6FI81.
PeptideAtlasiQ6FI81.
PRIDEiQ6FI81.
TopDownProteomicsiQ6FI81-1. [Q6FI81-1]

Protocols and materials databases

DNASUi57019.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394391; ENSP00000377914; ENSG00000005194. [Q6FI81-1]
ENST00000567518; ENSP00000456114; ENSG00000005194. [Q6FI81-3]
GeneIDi57019.
KEGGihsa:57019.
UCSCiuc002ell.2. human. [Q6FI81-1]

Organism-specific databases

CTDi57019.
DisGeNETi57019.
GeneCardsiCIAPIN1.
HGNCiHGNC:28050. CIAPIN1.
HPAiHPA041350.
HPA042182.
MIMi608943. gene.
neXtProtiNX_Q6FI81.
OpenTargetsiENSG00000005194.
PharmGKBiPA134978864.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4020. Eukaryota.
COG5636. LUCA.
GeneTreeiENSGT00390000011417.
HOGENOMiHOG000241118.
HOVERGENiHBG051104.
InParanoidiQ6FI81.
OMAiMWTLSAN.
OrthoDBiEOG091G0OTI.
PhylomeDBiQ6FI81.
TreeFamiTF314449.

Enzyme and pathway databases

ReactomeiR-HSA-2564830. Cytosolic iron-sulfur cluster assembly.

Miscellaneous databases

ChiTaRSiCIAPIN1. human.
EvolutionaryTraceiQ6FI81.
GeneWikiiCIAPIN1.
GenomeRNAii57019.
PROiQ6FI81.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005194.
CleanExiHS_CIAPIN1.
ExpressionAtlasiQ6FI81. baseline and differential.
GenevisibleiQ6FI81. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03115. Anamorsin. 1 hit.
InterProiIPR007785. Anamorsin.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13273. PTHR13273. 1 hit.
PfamiPF05093. CIAPIN1. 2 hits.
PF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCPIN1_HUMAN
AccessioniPrimary (citable) accession number: Q6FI81
Secondary accession number(s): A8K8B6
, O75206, O75207, Q9H0W1, Q9P1L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

'Ana-mors-in' means 'anti-death molecule' in Latin.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.