Q6FG10 (SYY_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	ACIAD0013

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02007
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity. HAMAP MF_02007
Subcellular location	Cytoplasm By similarity HAMAP MF_02007.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Tyrosine--tRNA ligase HAMAP MF_02007		PRO_0000236688
Regions
	Domain	342 – 402	61	S4 RNA-binding
	Motif	45 – 54	10	"HIGH" region HAMAP MF_02007
	Motif	229 – 233	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	232	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FG10 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 9B7E696A151BB4E3	FASTA	404	44,851
	10 20 30 40 50 60 MSNFLPAEEQ LALIQRGTHE IISEEDLLKK LKENRPLRIK AGFDPTAPDL HLGHTVLINK 70 80 90 100 110 120 LKAFQDLGHE VTFLIGDYTA MIGDPTGKSA TRPPLTREQV EANAKTYQEQ VFKILDPNKT 130 140 150 160 170 180 KVRFNSEWFN QRTAADLIQL ASQQTVSRML ERDDFTKRYN NHQPIAIHEF LYPLVQGYDS 190 200 210 220 230 240 IALEADVELG GTDQTFNLLM GRTLQGRYGQ ESQVCITVPI LEGLDGVNKM SKSLGNYIGV 250 260 270 280 290 300 FDAPGAMYQK VLSMPDTLIE RYFELLSFKS LDEIQGLLDE MANGRNPQDL KKILALELVE 310 320 330 340 350 360 RFHDADAAAN AHKGAGNIIT EGEIPEGTPE VTISRGEFGG EIFIASIVRL AGLTKNAAQA 370 380 390 400 KDAVSRGAVK VDWQVVDANF SVKENKTYLI QAGKKAIAQV TFTD

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ADP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG66997.1.
RefSeq	YP_044819.1. NC_005966.1.
3D structure databases
ProteinModelPortal	Q6FG10.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FG10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2880799.
GenomeReviews	Gene locus ACIAD0013 in contig CR543861_GR.
KEGG	aci:ACIAD0013.
NMPDR	fig|62977.3.peg.1079.
PATRIC	20738018. VBIAciSp98416_0011.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	YVVQVGK.
PhylomeDB	Q6FG10.
ProtClustDB	PRK05912.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0013-MONOMER.
Family and domain databases
HAMAP	MF_02007. Tyr_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024108. Tyr-tRNA-synth_bac_2. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_ACIAD

Accession

Primary (citable) accession number: Q6FG10

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program