Reviewed, UniProtKB/Swiss-Prot Q6FG03 (LSPA_ACIAD)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	ACIAD0021

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Lipoprotein signal peptidase HAMAP MF_00161		PRO_0000289348
Regions
	Transmembrane	10 – 30	21	Potential
	Transmembrane	48 – 68	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	101 – 121	21	Potential
	Transmembrane	141 – 161	21	Potential
Sites
	Active site	122	1	By similarity
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FG03-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 486C730BBC6042E0	FASTA	179	20,370
	10 20 30 40 50 60 MPKLEAKKGL FQFYPHNLIW LGLSILAIII DQWTKWIAST HLNYADPVPV LPFLNWTLLH 70 80 90 100 110 120 NYGAAFSFLS DAGGWQHYLF TGLAGIVSII FIFWLMRMPK NAMILPAAIA LILGGALGNL 130 140 150 160 170 IDRMTLGYVV DFIHIYYQNH HFPAFNIADS AITIGTILLL IDTFFLEKQR IQRAEVKHD

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG67004.1.
RefSeq	YP_044826.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FG03.
Protein family/group databases
MEROPS	A08.001.
Genome annotation databases
GeneID	2878606.
GenomeReviews	Gene locus ACIAD0021 in contig CR543861_GR.
KEGG	aci:ACIAD0021.
NMPDR	fig|62977.3.peg.1086.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FG03.
OMA	TKRIASE.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0021-MON.
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
ProDom	PD004304. Peptidase_A8. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_ACIAD

Accession

Primary (citable) accession number: Q6FG03

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)