SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6FG02

- SYI_ACIAD

UniProt

Q6FG02 - SYI_ACIAD

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, ACIAD0022
Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731Aminoacyl-adenylate By similarity
Binding sitei617 – 6171ATP By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciASP62977:GJVV-25-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:ACIAD0022
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
ProteomesiUP000000430: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098338Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi62977.ACIAD0022.

Structurei

3D structure databases

ProteinModelPortaliQ6FG02.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7711"HIGH" regionUniRule annotation
Add
BLAST
Motifi614 – 6185"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6FG02-1 [UniParc]FASTAAdd to Basket

« Hide

MSDKQTPENA VDYKATLNLP GTEFAMKANL AVREVKWLEE WYADNIYQKI    50
RASRIGKKKY VLHDGPPYAN GQIHLGHAVN KVLKDIIVKS RVMDGFDAPY 100
VPGWDCHGLP IELKVEEKVG KVGVKVDAST FRKACREYAL SQVDLQRKDF 150
IRMGVFGDWQ DPYLTMNFKQ EADIVRSLGE IQKAGHIEPG LKPVNWCIDC 200
GSALAEAEVE YEDKKSDAID VGFGVVDLND LSARLNVEVQ DPTDIVIWTT 250
TPWTLPANQA VALHAEIDYQ LVQVQSDRGT QNFILAKDLV ESAIARYKLE 300
NPVVLADFKG AVLELLQLQH PLLADRQVPV ILGEHVIATS GTGAVHTAPG 350
HGTDDYKVGL IYNLKVDNPV GGNGVYLPTA PIFSGEHIYK ANPQIIEALG 400
ATGRLWAHQP IVHSYPHCWR HKTPIIFRAT PQWFISMDAK GLRECALNAI 450
ENDISFVPDW GKNRIESMIE GRPDWCISRQ RTWGVPIPFF VHKDTNELHP 500
RTPELIEEVA QLIEHEGIDA WFNREAKDFI GADAEHYNAV RDTLDVWFDS 550
GTTHYAVLRQ REELTDPADL YLEGSDQHRG WFQSSLLTSI AINERAPYKG 600
LLTHGFVVDE KGRKMSKSLG NIITPQDIIK DMGADGLRFW IASSDYRYEM 650
TAGKEIFSRA SDGYRRIRNT LRFLLANLNG FTPSTDALPV DQLIALDQYI 700
LQRAAEVQKT VQQAYEDMNF HIVCSALTNF CINDLGGFYL DIIKDRQYTT 750
KADSAARHSA QTALYHLVQA FVRWMSPILS FTAQEAWPLI PEQTEQYVFT 800
TEWYDIPVAS TANLISEADW QTLISVKSAV NKQIEAARNA KLVGSNLSAK 850
VEIWAKDELQ SVLNQLGDEL RFVLITSQVN VYPYAEQGES TEMDGLRVQI 900
SAAEGEKCVR CWHVLPDVNT HADHPGLCGR CIINVTGSGE VRKYA 945
Length:945
Mass (Da):106,145
Last modified:July 19, 2004 - v1
Checksum:i7D400C9D4CB2EDF3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR543861 Genomic DNA. Translation: CAG67005.1.
RefSeqiYP_044827.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG67005; CAG67005; ACIAD0022.
GeneIDi2878563.
KEGGiaci:ACIAD0022.
PATRICi20738044. VBIAciSp98416_0019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR543861 Genomic DNA. Translation: CAG67005.1 .
RefSeqi YP_044827.1. NC_005966.1.

3D structure databases

ProteinModelPortali Q6FG02.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 62977.ACIAD0022.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG67005 ; CAG67005 ; ACIAD0022 .
GeneIDi 2878563.
KEGGi aci:ACIAD0022.
PATRICi 20738044. VBIAciSp98416_0019.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ASP62977:GJVV-25-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
    Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
    Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33305 / BD413 / ADP1.

Entry informationi

Entry nameiSYI_ACIAD
AccessioniPrimary (citable) accession number: Q6FG02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi