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Q6FG02

- SYI_ACIAD

UniProt

Q6FG02 - SYI_ACIAD

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731Aminoacyl-adenylateUniRule annotation
Binding sitei617 – 6171ATPUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciASP62977:GJVV-25-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ACIAD0022
OrganismiAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Taxonomic identifieri62977 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
ProteomesiUP000000430: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligasePRO_0000098338Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi62977.ACIAD0022.

Structurei

3D structure databases

ProteinModelPortaliQ6FG02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7711"HIGH" regionAdd
BLAST
Motifi614 – 6185"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6FG02-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDKQTPENA VDYKATLNLP GTEFAMKANL AVREVKWLEE WYADNIYQKI
60 70 80 90 100
RASRIGKKKY VLHDGPPYAN GQIHLGHAVN KVLKDIIVKS RVMDGFDAPY
110 120 130 140 150
VPGWDCHGLP IELKVEEKVG KVGVKVDAST FRKACREYAL SQVDLQRKDF
160 170 180 190 200
IRMGVFGDWQ DPYLTMNFKQ EADIVRSLGE IQKAGHIEPG LKPVNWCIDC
210 220 230 240 250
GSALAEAEVE YEDKKSDAID VGFGVVDLND LSARLNVEVQ DPTDIVIWTT
260 270 280 290 300
TPWTLPANQA VALHAEIDYQ LVQVQSDRGT QNFILAKDLV ESAIARYKLE
310 320 330 340 350
NPVVLADFKG AVLELLQLQH PLLADRQVPV ILGEHVIATS GTGAVHTAPG
360 370 380 390 400
HGTDDYKVGL IYNLKVDNPV GGNGVYLPTA PIFSGEHIYK ANPQIIEALG
410 420 430 440 450
ATGRLWAHQP IVHSYPHCWR HKTPIIFRAT PQWFISMDAK GLRECALNAI
460 470 480 490 500
ENDISFVPDW GKNRIESMIE GRPDWCISRQ RTWGVPIPFF VHKDTNELHP
510 520 530 540 550
RTPELIEEVA QLIEHEGIDA WFNREAKDFI GADAEHYNAV RDTLDVWFDS
560 570 580 590 600
GTTHYAVLRQ REELTDPADL YLEGSDQHRG WFQSSLLTSI AINERAPYKG
610 620 630 640 650
LLTHGFVVDE KGRKMSKSLG NIITPQDIIK DMGADGLRFW IASSDYRYEM
660 670 680 690 700
TAGKEIFSRA SDGYRRIRNT LRFLLANLNG FTPSTDALPV DQLIALDQYI
710 720 730 740 750
LQRAAEVQKT VQQAYEDMNF HIVCSALTNF CINDLGGFYL DIIKDRQYTT
760 770 780 790 800
KADSAARHSA QTALYHLVQA FVRWMSPILS FTAQEAWPLI PEQTEQYVFT
810 820 830 840 850
TEWYDIPVAS TANLISEADW QTLISVKSAV NKQIEAARNA KLVGSNLSAK
860 870 880 890 900
VEIWAKDELQ SVLNQLGDEL RFVLITSQVN VYPYAEQGES TEMDGLRVQI
910 920 930 940
SAAEGEKCVR CWHVLPDVNT HADHPGLCGR CIINVTGSGE VRKYA
Length:945
Mass (Da):106,145
Last modified:July 19, 2004 - v1
Checksum:i7D400C9D4CB2EDF3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR543861 Genomic DNA. Translation: CAG67005.1.
RefSeqiYP_044827.1. NC_005966.1.

Genome annotation databases

EnsemblBacteriaiCAG67005; CAG67005; ACIAD0022.
GeneIDi2878563.
KEGGiaci:ACIAD0022.
PATRICi20738044. VBIAciSp98416_0019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR543861 Genomic DNA. Translation: CAG67005.1 .
RefSeqi YP_044827.1. NC_005966.1.

3D structure databases

ProteinModelPortali Q6FG02.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 62977.ACIAD0022.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG67005 ; CAG67005 ; ACIAD0022 .
GeneIDi 2878563.
KEGGi aci:ACIAD0022.
PATRICi 20738044. VBIAciSp98416_0019.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ASP62977:GJVV-25-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
    Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
    Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33305 / BD413 / ADP1.

Entry informationi

Entry nameiSYI_ACIAD
AccessioniPrimary (citable) accession number: Q6FG02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3