Q6FFN2 (GUAA_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	ACIAD0151

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000229396
Regions
	Domain	39 – 232	194	Glutamine amidotransferase type-1
	Domain	233 – 425	193	GMPS ATP-PPase
	Nucleotide binding	260 – 266	7	ATP By similarity
Sites
	Active site	116	1	Nucleophile By similarity
	Active site	206	1	By similarity
	Active site	208	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FFN2 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: B7CEF0B74157E965	FASTA	550	61,032
	10 20 30 40 50 60 MLIERTCAIL GKMLEYCASL VALHSCVIMT TNTHITDDRI LILDFGSQYS QLIARRVREA 70 80 90 100 110 120 GVYSEMYAFD MSEEDIRAFN PNGIILSGGP ESVHEEGSPR APQVVFELGV PVLGICYGFQ 130 140 150 160 170 180 TMSEQLGGKV EPGTVHEFGY AEVDIQQRDQ LVGNLQDREN QLHVWMSHGD KVSRLPEGFS 190 200 210 220 230 240 TTASTPSCPF AAASDEARRF YGVQFHPEVT HTAKGAELLA NFVHKICGCG GLWTPEHIID 250 260 270 280 290 300 LRVEQLRAQI GDEKVLLGLS GGVDSSVVAA LLHKAIGDQL TCVFVDNGLL RLNEGDQVMQ 310 320 330 340 350 360 MFADNMGIRV IRADAEERFL TALAGEVDPE KKRKIIGREF IEVFAEEARK LDGVKFLAQG 370 380 390 400 410 420 TIYPDVIESA ASKQGKAHVI KSHHNVGGLP DDLEFELVEP LRDLFKDEVR KLGTTLGLPH 430 440 450 460 470 480 SMIYRHPFPG PGLGVRILGE VKKEYADILR LADDIFMQEL RASGWYDKTA QAFAVFQPVK 490 500 510 520 530 540 SVGVVGDGRR YAWVIALRAV ETVDFMTARF AHLPYELVDK ISTRIMNEIK DVSRVVYDVS 550 SKPPATIEWE

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ADP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG67125.1.
RefSeq	YP_044947.1. NC_005966.1.
3D structure databases
ProteinModelPortal	Q6FFN2.
SMR	Q6FFN2. Positions 34-550.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FFN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2879247.
GenomeReviews	Gene locus ACIAD0151 in contig CR543861_GR.
KEGG	aci:ACIAD0151.
NMPDR	fig|62977.3.peg.181.
PATRIC	20738278. VBIAciSp98416_0134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0519.
HOGENOM	HBG391775.
OMA	DGRTYEY.
PhylomeDB	Q6FFN2.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0151-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_ACIAD

Accession

Primary (citable) accession number: Q6FFN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program