Reviewed, UniProtKB/Swiss-Prot Q6FFL8 (MAO1_ACIAD)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	ACIAD0166

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	NAD-dependent malic enzyme		PRO_0000160212
Sites
	Active site	105	1	Proton donor By similarity
	Active site	176	1	Proton acceptor By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	248	1	Divalent metal cation By similarity
	Metal binding	271	1	Divalent metal cation By similarity
	Binding site	158	1	NAD By similarity
	Binding site	271	1	NAD By similarity
	Binding site	419	1	NAD By similarity
	Site	271	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FFL8-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 2122BE03FE5FFC65	FASTA	566	62,969
	10 20 30 40 50 60 MTAETNTNKH PLYIPYAGYT LLELPLLNKG SAFTQEERSH FNLHGLIPHV IETIEEQSQR 70 80 90 100 110 120 SYQQYGAFND DINKHIYLRN IQDTNETLFY RLINDHLEEM MPIIYTPTVG EACQRFSDIY 130 140 150 160 170 180 RRHRGIFISY PDREHIDDIL QNVSKRNVKV IVITDGERIL GLGDQGIGGM GIPIGKLSLY 190 200 210 220 230 240 TACGGISPAY TLPITIDVGT NNQQLLNDPI YMGWRQPRIS GDEYYEFIDQ VLTGIRRRWP 250 260 270 280 290 300 HALIQFEDFA QKNAMPLLTK YRDKFCCFND DIQGTAAVSV GSLIAASRAA GKQLKDQTIT 310 320 330 340 350 360 FLGAGSAGCG IAEQIVAQMV AEGLTDAQAR ARVYMVDRFG LITENQPNLL DFQRKLAQKA 370 380 390 400 410 420 EVVSDWGNVE EVISLLDVVK NAKPTVLIGV SGQPGLFTEE VIRTLAENCE RPIVMPLSNP 430 440 450 460 470 480 TSRVEALPSD VIQWTNGRAL IATGSPFAPV NYQGKLYNIS QCNNSYIFPG IGLGVIASGA 490 500 510 520 530 540 KRVTDNMLMA SSNALADCSP LLQNPNADLL PAIADIQDVS KRIAFKVAKA AIEDGVALNM 550 560 SDEVLLQNIE KEFWKPKYRG YKRVPF

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG67139.1.
RefSeq	YP_044961.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2879295.
GenomeReviews	Gene locus ACIAD0166 in contig CR543861_GR.
KEGG	aci:ACIAD0166.
NMPDR	fig|62977.3.peg.256.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FFL8.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0166-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_ACIAD

Accession

Primary (citable) accession number: Q6FFL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)