Reviewed, UniProtKB/Swiss-Prot Q6FEN5 (PROA_ACIAD)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	ACIAD0547

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189681

Sequences

	Sequence		Length	Mass (Da)
	Q6FEN5-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 6086636708C835DF	FASTA	421	46,059
	10 20 30 40 50 60 MQDSIEQYMQ AVGQQARKAS RVLSSASTHT KNNALSAIYT ALVNHEPEIL AANAVDMQKG 70 80 90 100 110 120 RDSHLDSALL DRLELTPTRF KGMLQGLKDV IALTDPIGEI TDLAYRPSGI QLGKMRVPLG 130 140 150 160 170 180 VIGMIYESRP NVTLEAASLA LKSGNAIILR GGSEALQSNK AIATAIQHGL KVAGLPEHAI 190 200 210 220 230 240 QVIETADRAA VGQLITMTEY VDVIVPRGGK SLIERISNEA RIPVIKHLDG NCHVFIDVEA 250 260 270 280 290 300 DLHKALPIAL NAKTHRYGVC NAMETLLVHE EIAEEFLPRI AELYAEKHVE LRGCQETRRI 310 320 330 340 350 360 LGSSVKTATE EDWYTEYLAP ILAIKVVEHI DQAIEHINKY GSHHTDAIVT ENYSKVRKFL 370 380 390 400 410 420 AEVDSSSVMI NASTRFADGF EYGLGAEIGI STDKIHARGP VGLEGLTSQK WIVFGDGQIR H

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG67473.1.
RefSeq	YP_045295.1.
3D structure databases
SMR	Q6FEN5. Positions 9-421.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FEN5.
Genome annotation databases
GeneID	2880045.
GenomeReviews	Gene locus ACIAD0547 in contig CR543861_GR.
KEGG	aci:ACIAD0547.
NMPDR	fig|62977.3.peg.34.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HBG318080.
OMA	ISMPEYV.
PhylomeDB	Q6FEN5.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0547-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACIAD

Accession

Primary (citable) accession number: Q6FEN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)