Reviewed, UniProtKB/Swiss-Prot Q6FEJ4 (TGT_ACIAD)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	ACIAD0590

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135439
Sites
	Active site	99	1	Nucleophile By similarity
	Metal binding	313	1	Zinc By similarity
	Metal binding	315	1	Zinc By similarity
	Metal binding	318	1	Zinc By similarity
	Metal binding	344	1	Zinc By similarity
	Binding site	100	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FEJ4-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 49D9DD15D9310DC1	FASTA	386	43,951
	10 20 30 40 50 60 MFFRLDLESC MKFEKLGQSG RARRGRLTLA HGVVETPVFM PVGTYGTVKG MLPRDIEEIQ 70 80 90 100 110 120 AQIILGNTFH LYLRPGLEVI KEHGGLHEFI KWDKPILTDS GGFQVFSLGA MRKIKEEGVT 130 140 150 160 170 180 FRSPIDGSKV FLSPEISMEI QQVLNSDIVM IFDECTPYPA THEEAQKSLQ LSLRWAKRCK 190 200 210 220 230 240 THHHEQLNNT NALFGIIQGG MYEDLRDESL NGLLEIGFDG YAIGGLSVGE PKEEMIKVLD 250 260 270 280 290 300 YLPAKMPEDK PRYLMGVGKP EDIVEAVRRG VDMFDCVMPT RNARNGHYFV TDGLVRIRNS 310 320 330 340 350 360 KYRHDQSPLD PHCDCYTCKN FTRAYLYHLE KCGEMLASML GTIHNLRYYQ RLTQGIRDAL 370 380 EQGTFDEFVT DFYTRRGLDV PPAPVD

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG67514.1.
RefSeq	YP_045336.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2881193.
GenomeReviews	Gene locus ACIAD0590 in contig CR543861_GR.
KEGG	aci:ACIAD0590.
NMPDR	fig|62977.3.peg.128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FEJ4.
OMA	Q6FEJ4. VLNSDIV.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0590-MON.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_ACIAD

Accession

Primary (citable) accession number: Q6FEJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)