Reviewed, UniProtKB/Swiss-Prot Q6FE36 (SYM_ACIAD)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	ACIAD0768

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00098
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 685	685	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139096
Regions
	Domain	584 – 685	102	tRNA-binding
	Motif	330 – 334	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	142	1	Zinc By similarity
	Metal binding	145	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity
	Metal binding	158	1	Zinc By similarity
	Binding site	333	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FE36-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 6B7D897255153C6C	FASTA	685	76,745
	10 20 30 40 50 60 MRKILVTNAL PYANGPIHMG HLLGYIQADI WVRAMRAMGH DVTYVCADDA HGTAIMLRAE 70 80 90 100 110 120 ANGISPEKQI ANVQQEHIRD FDGFGVHFDH YDSTHSAENK VRSEDIYLKN REAGNIAVRP 130 140 150 160 170 180 ITQLFDPEKQ MFLSDRFIKG TCPKCKAEDQ YGDACEVCGT TYNATELLNP RSTLSGATPV 190 200 210 220 230 240 EKSSDHYFFK LPNFGEYLQK WTRDEGRLPV SIANKLDEWF EAGLADWDIS RDAPYFGFEI 250 260 270 280 290 300 PDAPNKYFYV WVDAPIGYMS SFENYIKAKR PELNFDDYWK KDSQNEVYHF IGKDIVYFHA 310 320 330 340 350 360 LFWPAMLEGA NYRTPSGLFV NGFLTVNGQK MSKSRGTFIK AETYLQHLNP EYLRYYFASK 370 380 390 400 410 420 LSDKVEDSDL NLDDFVQKVN SDLVGKVVNI ASRCAKFINS SFNNTLSANC AEPELVQSFI 430 440 450 460 470 480 DAGDSIAQAY EAREFSAAIR EIMALADKAN QYIDEKKPWA LAKQEGQEQQ VHNVCSVGIN 490 500 510 520 530 540 LFRQLAIYLS PVLPTLAAQV QAFLQLESFD FASRQQILLA HEIAQFQPLM QRVDPKAVAA 550 560 570 580 590 600 MVDASKDSLA ATAEAPKAEK KKEKKVEKKP EPKVGEAEII GIEDFIKVDL RVALVQEAAT 610 620 630 640 650 660 VEGSDKLLQL TLDVGEAEPR NVFSGIREFY QPEDLKGKLV VMVANLAPRK MRFGISNGMV 670 680 LAAGNGDGVW VISPDSGAKP GDKVS

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG67672.1.
RefSeq	YP_045494.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FE36.
Genome annotation databases
GeneID	2879488.
GenomeReviews	Gene locus ACIAD0768 in contig CR543861_GR.
KEGG	aci:ACIAD0768.
NMPDR	fig|62977.3.peg.573.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FE36.
OMA	CEAVPES.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0768-MON.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ACIAD

Accession

Primary (citable) accession number: Q6FE36

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)