Reviewed, UniProtKB/Swiss-Prot Q6FDH0 (ASPD_ACIAD)

Names and origin

Protein names

Recommended name:
    Probable L-aspartate dehydrogenase
    EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	ACIAD0997

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity.
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity.
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_0000144879
Sites
	Active site	216	1	By similarity
	Binding site	120	1	NAD; via amide nitrogen By similarity
	Binding site	186	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FDH0-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 9AB52FEC3BA88672	FASTA	263	28,221
	10 20 30 40 50 60 MKQLMMIGFG AMASEVYAHL PQDLELKWIV VPERSVESVK QKVDRHIQVI SDINQCDGAP 70 80 90 100 110 120 DYVIEVAGQA AVKEHAKNVL AHGWNIGLIS VGTLADSEFF TELQQTAEQN GAHLHLLAGA 130 140 150 160 170 180 IAGIDGIAAA KEGGLEKVTY KGCKSPNSWR GSYAEQLIDL DQVHTVTMFY RGTAREAAQK 190 200 210 220 230 240 FPANANVAAT IALAGVGMDN TIVELTVDPD TTQNKHTIVA EGRFGQMTIE MVGVPLASNP 250 260 KTSTLAALSV IRACRNSVEA IQI

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG67888.1.
RefSeq	YP_045710.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FDH0.
Genome annotation databases
GeneID	2880590.
GenomeReviews	Gene locus ACIAD0997 in contig CR543861_GR.
KEGG	aci:ACIAD0997.
NMPDR	fig|62977.3.peg.1048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FDH0.
OMA	ECAGHSA.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD0997-MON.
Family and domain databases
HAMAP	MF_01265. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. [Graphical view]
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProDom	PD017325. Asp_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ASPD_ACIAD

Accession

Primary (citable) accession number: Q6FDH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)