Reviewed, UniProtKB/Swiss-Prot Q6FD29 (PYRC_ACIAD)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	ACIAD1150

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Dihydroorotase		PRO_1000024007
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	247	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FD29-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: DE11304473479E6E	FASTA	344	38,867
	10 20 30 40 50 60 MDTITLLQPD DWHAHLRDGL ALKRTVPDLA QQFARAICMP NLVPPVKTVD EAEAYRERIM 70 80 90 100 110 120 AHVPEGVHFD PRMVLYFTDH TSPSEVKKIK DSAHVNAIKL YPAGATTNSD NGVSDIRKVY 130 140 150 160 170 180 AVIEQLEEHQ VPLLLHGEVT HHHVDIFDRE KRFLDEVLSP LLKQFPKLKL VLEHITTSEA 190 200 210 220 230 240 AHFVLEQDRN VAATITPQHL LFNRNDMLVG GIKPHFYCLP ILKRQTHQQT LIEVATSGNP 250 260 270 280 290 300 KFFLGTDSAP HSKNAKENAC GCAGCYSAPT AIELYAQAFD QVNKIERLEG FASHFGADFY 310 320 330 340 GLPRNTNTIT LVKEDQIIPE QLDYLDDEKI IPLYAGKTIQ WRKV

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG68030.1.
RefSeq	YP_045852.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2879564.
GenomeReviews	Gene locus ACIAD1150 in contig CR543861_GR.
KEGG	aci:ACIAD1150.
NMPDR	fig|62977.3.peg.65.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FD29.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1150-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ACIAD

Accession

Primary (citable) accession number: Q6FD29

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)