Reviewed, UniProtKB/Swiss-Prot Q6FCS7 (PURA_ACIAD)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	ACIAD1258

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000224247
Regions
	Nucleotide binding	13 – 19	7	GTP Potential
Sites
	Active site	141	1	By similarity
	Active site	148	1	By similarity
	Metal binding	14	1	Magnesium By similarity
	Metal binding	41	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FCS7-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 6390A56B8EF9918A	FASTA	439	47,238
	10 20 30 40 50 60 MGKNVVVLGT QWGDEGKGKI VDLLTDQAAA VVRYQGGHNA GHTLVVGGKK TVLHLIPSGI 70 80 90 100 110 120 LRENVLCLIG NGVVLSPAAL IKEMGILEEE GVPVKERLRI SPNCPLILPN HIALDQAREK 130 140 150 160 170 180 KRGNAKIGTT GRGIGPAYED KVARRAVRVA DLVRGGAALE EKLQEMLELH NFQLTQFYGV 190 200 210 220 230 240 EAVKFEDVLA LCNEWREVLA PLVIDVTKVL HDYRKEGKAI MFEGAQGSLL DIDHGTYPYV 250 260 270 280 290 300 TSSNTTAGGV SSGSGMGPLH LDYVLGITKA YTTRVGAGPF PTELHYDAAT DTGDAIGRHL 310 320 330 340 350 360 GTVGHEFGAS TGRQRRCGWF DAEILRRSVE VNSLSGICLT KLDVLDGLDE IKICVGYEDV 370 380 390 400 410 420 DSGCAGSSDA VSFESLKPIY ETMPGWSEST VGLTSIDQLP ANALAYVKRI EQLIECPIDI 430 ISTGPDRAET MILRHPFSA

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG68132.1.
RefSeq	YP_045954.1.
3D structure databases
SMR	Q6FCS7. Positions 2-439.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FCS7.
Genome annotation databases
GeneID	2879345.
GenomeReviews	Gene locus ACIAD1258 in contig CR543861_GR.
KEGG	aci:ACIAD1258.
NMPDR	fig|62977.3.peg.327.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0104.
HOGENOM	HBG658237.
OMA	YVLGIIK.
PhylomeDB	Q6FCS7.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1258-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ACIAD

Accession

Primary (citable) accession number: Q6FCS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)