Reviewed, UniProtKB/Swiss-Prot Q6FCR9 (ILVD1_ACIAD)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 1
      Short name=DAD 1
    EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	ACIAD1266

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000225362
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6FCR9-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 1ADC2552A334C54C	FASTA	609	65,404
	10 20 30 40 50 60 MPDYRSKTST HGRNMAGARG LWRATGMKDE DFGKPIIAVV NSFTQFVPGH VHLKDLGQMV 70 80 90 100 110 120 AEQIQAAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRDLI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPV VFVSGGPMEA GKVKFRGNEK AIDLVDAMVV AADDSYTDEE 190 200 210 220 230 240 VQAFERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS IVATHANRKK LFLKAGELIV 250 260 270 280 290 300 SLAKRYYEQD DSSILPRSIA TKAAYENAMT LDIAMGGSTN TVLHLLAAAS EAEVDFTMDD 310 320 330 340 350 360 IDRLSRNVPV LCKVAPAKQD VHMEDVHRAG GIMSILGELD RAKLLNTTVS TVHEKTLQDA 370 380 390 400 410 420 LNKWDIIRTE DADVYEFFRS SPGGVPTQVA FSQNRYYSTL DGDRENGVIR NAEHAFSKDG 430 440 450 460 470 480 GLAVLYGNIA LEGCIVKTAG VDESILKFNG TARVFESQDA AVEAILGNEI KAGDVVVIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKDCAL ITDGRFSGGS SGLSIGHISP EAAEGGAIGL 550 560 570 580 590 600 VEDGDLIEID IPNRSMNLKV DDETLAARRK AQDEKGWKPV EERKRKVSKA LKVYAMHTTS AAKGAVRIL

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG68140.1.
RefSeq	YP_045962.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FCR9.
Genome annotation databases
GeneID	2877811.
GenomeReviews	Gene locus ACIAD1266 in contig CR543861_GR.
KEGG	aci:ACIAD1266.
NMPDR	fig|62977.3.peg.391.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FCR9.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1266-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_ACIAD

Accession

Primary (citable) accession number: Q6FCR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)