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Q6FCN6 (TRMB_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:ACIAD1302
OrganismAcinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) [Complete proteome] [HAMAP]
Taxonomic identifier62977 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171283

Regions

Region222 – 2254Substrate binding By similarity

Sites

Active site1491 By similarity
Binding site751S-adenosyl-L-methionine By similarity
Binding site1001S-adenosyl-L-methionine By similarity
Binding site1271S-adenosyl-L-methionine By similarity
Binding site1491S-adenosyl-L-methionine By similarity
Binding site1531Substrate By similarity
Binding site1851Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6FCN6 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 29BA34A4C55974E3

FASTA24528,398
        10         20         30         40         50         60 
MSHTKIMSTD QLETQITELD NLPEHREIVT FMRRSAPLNT SQRSALEQYQ DLILEYPVGD 

        70         80         90        100        110        120 
LRQHFEHPEH PLTVEIGFGM GRSLVLMAKA HPERNFVGIE VHVPGIAQCV YEAGMAGLKN 

       130        140        150        160        170        180 
LRVLDADAIQ VLREMPDNSI NCVQLYFPDP WQKKRHFKRR FVIHERMQLV EQKLELGGTF 

       190        200        210        220        230        240 
HAATDWEPYA EWMLDVLDNR PALENLAGKG NSYPRPEWRP QTKFERRGIE SGHKINDFIF 


KKIGS 

« Hide

References

[1]"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33305 / BD413 / ADP1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR543861 Genomic DNA. Translation: CAG68173.1.
RefSeqYP_045995.1. NC_005966.1.

3D structure databases

ProteinModelPortalQ6FCN6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING62977.ACIAD1302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAG68173; CAG68173; ACIAD1302.
GeneID2880729.
KEGGaci:ACIAD1302.
PATRIC20740423. VBIAciSp98416_1170.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycASP62977:GJVV-1230-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ACIAD
AccessionPrimary (citable) accession number: Q6FCN6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways