Reviewed, UniProtKB/Swiss-Prot Q6FCL9 (PYRD_ACIAD)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	ACIAD1321

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00225
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity. HAMAP MF_00225
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_0000148420
Sites
	Active site	172	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FCL9-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 34E542D27E5FAE47	FASTA	334	36,308
	10 20 30 40 50 60 MLYSLARPML FSLAPERAHE LTLSMLKTAH KMGMLRQTIQ PKPVTCMGIE FPNPVGLAAG 70 80 90 100 110 120 LDKNGAYIDA LAGLGFGFIE IGTITPRPQS GNPKPRLFRI PEAKAIINRM GFNNEGVDQL 130 140 150 160 170 180 VENVKAAKFR GILGINIGKN ADTPVENAVD DYLICLEKVY NYASYITVNI SSPNTKNLRS 190 200 210 220 230 240 LQSGDALTEL LETLKKRQLE LAEQYHHYVP LVLKVAPDLT HEDIQFISEQ LLTFKIDGLI 250 260 270 280 290 300 VTNTTLSREG VENLPFGNEA GGLSGAPVFE KSTECLRSFA KVLNDQIPLI GVGGITQGEH 310 320 330 AVAKKDAGAS LVQIYSGLIY TGPDLIKECV SAIT

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG68191.1.
RefSeq	YP_046013.1.
3D structure databases
SMR	Q6FCL9. Positions 1-333.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FCL9.
Genome annotation databases
GeneID	2879565.
GenomeReviews	Gene locus ACIAD1321 in contig CR543861_GR.
KEGG	aci:ACIAD1321.
NMPDR	fig|62977.3.peg.495.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0167.
HOGENOM	HBG351027.
OMA	NAEQQGG.
PhylomeDB	Q6FCL9.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1321-MONOMER.
Family and domain databases
HAMAP	MF_00225. DHO_dh_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ACIAD

Accession

Primary (citable) accession number: Q6FCL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)