Q6FAZ1 (MURB_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	ACIAD1945

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224651
Regions
	Domain	18 – 188	171	FAD-binding PCMH-type
Sites
	Active site	165	1	By similarity
	Active site	234	1	Proton donor By similarity
	Active site	330	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FAZ1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 1DFC8E539F67313E	FASTA	345	39,023
	10 20 30 40 50 60 MIHIQHQVQL KPFNTLNLDV VASHYIKVQS VNELIEALDY AEQQQLNALI LSGGSNMLLP 70 80 90 100 110 120 QSLDALVLHI DIQGLEMIDE DSESVKIKVG GGQIWHDFVL MTTQKGWYGL QNLALIPGLV 130 140 150 160 170 180 GASPVQNIGA YGVEVGEFIE LVHVYDRSEK SVKTISCADC KFAYRHSIFK DEPYRYIITH 190 200 210 220 230 240 VTFKLFKTPN LKLSYGDLLK AVGNEQTPEN LQKQVIQIRE SKLPNPKEYP NVGSFFKNPV 250 260 270 280 290 300 ISRQDFNQLL TEFPLIPHYP QPHDRVKIAA GWLIDQAGWK GKRLGAVGMF EKQALVLVNY 310 320 330 340 ANATLIDVQH TYRHVQSDIQ QKFKLRLEPE PVLFGSNGLI LSHME

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ADP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG68772.1.
RefSeq	YP_046594.1. NC_005966.1.
3D structure databases
ProteinModelPortal	Q6FAZ1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FAZ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2879154.
GenomeReviews	Gene locus ACIAD1945 in contig CR543861_GR.
KEGG	aci:ACIAD1945.
NMPDR	fig|62977.3.peg.1856.
PATRIC	20741618. VBIAciSp98416_1754.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	IEQAGWK.
PhylomeDB	Q6FAZ1.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD1945-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_ACIAD

Accession

Primary (citable) accession number: Q6FAZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program