Reviewed, UniProtKB/Swiss-Prot Q6FA61 (QUEF_ACIAD)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	ACIAD2261

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.
Pathway	tRNA modification; queuosine-tRNA biosynthesis.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	NADPH-dependent 7-cyano-7-deazaguanine reductase		PRO_0000163018

Sequences

	Sequence		Length	Mass (Da)
	Q6FA61-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: F031B8094A96E2EA	FASTA	271	31,042
	10 20 30 40 50 60 MSVEQSLLGK ETEYPTTYSP SVLFPISRDA AREKYAQIEG ISQGKDWWHV FELSWLNSDN 70 80 90 100 110 120 IPQVAIGRIT LPATSPYLIE SKSLKLYFNS LNFHVFASKQ ELIDLVEKDL SQAANAKVSL 130 140 150 160 170 180 ELFDVDSLEI SKPSGMCIDD LKPERLESHP DASLLKLDAN EQHDADVTLY SHLLRSNCPV 190 200 210 220 230 240 TGQPDWGTVF IRYTGRKHCY RSILAYIISY RQHNGFHEQC VEQIYADIWK NLQPEKLMVY 250 260 270 ATYTRRGGLD INPCRVSDLS WMPNPIRLAR Q

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG69052.1.
RefSeq	YP_046874.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2880015.
GenomeReviews	Gene locus ACIAD2261 in contig CR543861_GR.
KEGG	aci:ACIAD2261.
NMPDR	fig|62977.3.peg.2161.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FA61.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD2261-MON.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycOHase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_ACIAD

Accession

Primary (citable) accession number: Q6FA61

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)