Reviewed, UniProtKB/Swiss-Prot Q6F9D9 (PROB_ACIAD)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	ACIAD2560

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109627
Regions
	Domain	285 – 363	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q6F9D9-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: C2C3DB863B9075C6	FASTA	377	40,717
	10 20 30 40 50 60 MIEVIDGQRQ LKACKRIVVK IGSSLLTANG QGLDLDAISH WAMQIADLHN AGHEIILVSS 70 80 90 100 110 120 GAVAEGMVRM KLTSRPTDLP SLQACAAIGQ MGLIHTWSSV LEKHTIQTAQ VLLTHDDLAD 130 140 150 160 170 180 RRRYLNSCDA LQNLIEWRVI PVINENDTVS TDEIRFGDND TLAAMVAGQV HADLLIILTD 190 200 210 220 230 240 QQGMFDSDPR SNPNAKLLTT VNAMDDALFE MAGGGGVLGR GGMVTKVRAA RLAAKSGCPT 250 260 270 280 290 300 LIASGESDHV LSRLMSGELL GTLFSTDKDR ITAHQQWLAA HLQTAGRLVI DAGAVEAIKQ 310 320 330 340 350 360 RHRSLLPVGV KAVEGHFNRG DVVECVDQSG QRVAVGRVNF SSRSAEIIKG LASDKVHQVL 370 GEARSLEMIH RNHMAIY

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG69325.1.
RefSeq	YP_047147.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6F9D9.
Genome annotation databases
GeneID	2880046.
GenomeReviews	Gene locus ACIAD2560 in contig CR543861_GR.
KEGG	aci:ACIAD2560.
NMPDR	fig|62977.3.peg.2340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6F9D9.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD2560-MON.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ACIAD

Accession

Primary (citable) accession number: Q6F9D9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)