Q6F943 (PDXB_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Erythronate-4-phosphate dehydrogenase
EC=1.1.1.290

Gene names

Name:	pdxB
Ordered Locus Names:	ACIAD2667

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate By similarity. HAMAP-Rule MF_01825
Catalytic activity	4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH. HAMAP-Rule MF_01825
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 2/5. HAMAP-Rule MF_01825
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01825.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-phosphoerythronate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Erythronate-4-phosphate dehydrogenase HAMAP-Rule MF_01825		PRO_0000297429
Sites
	Active site	206	1	By similarity
	Active site	234	1	By similarity
	Active site	251	1	Proton donor By similarity
	Binding site	45	1	Substrate By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	146	1	NAD By similarity
	Binding site	229	1	NAD By similarity
	Binding site	254	1	NAD; via amide nitrogen By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F943 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: CD56F8D95E2DBF3A	FASTA	355	39,464
	10 20 30 40 50 60 MKIVADENLA FTDYFFSEFG EIQHRAGRLL TAHDVSDAEA LLVRSVTKVN QALIEHSQLK 70 80 90 100 110 120 FVGSATIGTD HLDISALQQQ DILWSNAPGC NAQAVAEYVI TALYHLDSDV FERGQDFTLG 130 140 150 160 170 180 IIGLGNVGRR LAKMAALLGW NVIGCDPFVQ LPDIHNLSFD DVLQKSDAIS VHVPLTHSGS 190 200 210 220 230 240 HPTFHLFDQH AFASMPASTI LINSARGPVI EEQALIQDIY QTGRKVVLDV FEHEPVISEQ 250 260 270 280 290 300 LLDVVNLVTP HIAGYSLEGK ARGTQMIYDA FCKVFGYEAS KKFETQLPVC EPFFQQQDLK 310 320 330 340 350 QILKAHLRAI YDIAQDDHNL RACVKDGQVD QCAFDQLRKE YPLRREWAAH GGPVA

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ADP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG69422.1.
RefSeq	YP_047244.1. NC_005966.1.
3D structure databases
HSSP	HSSP built from PDB template 1SC6 based on UniProtKB P0A9T0.
ProteinModelPortal	Q6F943.
ModBase	Search...
Protein-protein interaction databases
STRING	62977.ACIAD2667.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG69422; CAG69422; ACIAD2667.
GeneID	2878298.
KEGG	aci:ACIAD2667.
PATRIC	20742933. VBIAciSp98416_2407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0111.
HOGENOM	HOG000234432.
KO	K03473.
OMA	SSAPGCN.
ProtClustDB	CLSK707643.
Enzyme and pathway databases
BioCyc	ASP62977:GJVV-2481-MONOMER.
UniPathway	UPA00244; UER00310.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
HAMAP	MF_01825. PdxB.
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR020921. Erythronate-4-P_DHase. IPR024531. Erythronate-4-P_DHase_dimer. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10996:SF4. PTHR10996:SF4. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF11890. DUF3410. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDXB_ACIAD

Accession

Primary (citable) accession number: Q6F943

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program