Reviewed, UniProtKB/Swiss-Prot Q6F8M7 (CARA_ACIAD)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	ACIAD2860

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112242
Regions
	Domain	192 – 379	188	Glutamine amidotransferase type-1
	Region	1 – 188	188	CPSase HAMAP MF_01209
Sites
	Active site	269	1	Nucleophile By similarity
	Active site	353	1	By similarity
	Active site	355	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F8M7-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 944FE433D558FB46	FASTA	379	40,851
	10 20 30 40 50 60 MSTPAILALA DGTIFKGTSI GATGSTTGEV VFNTAMTGYQ EILTDPSYAQ QLVTLTYPHI 70 80 90 100 110 120 GNTGCNEEDT ESGRIHKVWA NGLIIRDLPL LHSNFRSEQS LAEYLIQHNV VAIADIDTRK 130 140 150 160 170 180 LTRILRDKGA QNGCILAGEN ITEEEALAKA RAFGGLNGLD LAKECCDPNG FEWTEGSWEL 190 200 210 220 230 240 GKGFTQPELK YHVVAYDYGV KTNILRMLAD RGCKLTVVPA QTPAEQVLAL NPDGVFLSNG 250 260 270 280 290 300 PGDPAACDYA IEAVKTIVET TNLPVFGICL GHQILALASG AKTMKMNHGH HGANHPVQNL 310 320 330 340 350 360 EQGTVMITSQ NHGFAVDEST LPANLKVTHR SLFDGTNQGI HRTDKPAFSF QGHPEASPGP 370 HDCAPLFDHF IELIEAAKK

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG69588.1.
RefSeq	YP_047410.1.
3D structure databases
SMR	Q6F8M7. Positions 5-379.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6F8M7.
Genome annotation databases
GeneID	2880525.
GenomeReviews	Gene locus ACIAD2860 in contig CR543861_GR.
KEGG	aci:ACIAD2860.
NMPDR	fig|62977.3.peg.2603.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0505.
HOGENOM	HBG286341.
OMA	FTYPELG.
PhylomeDB	Q6F8M7.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD2860-MONOMER.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_ACIAD

Accession

Primary (citable) accession number: Q6F8M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)