Q6F872 (SYFA_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	ACIAD3042

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000126654
Sites
	Metal binding	255	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F872 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: A538F98CD0A91A64	FASTA	330	37,153
	10 20 30 40 50 60 MRVTMSLEAL TTEALAAIAA AQDLAALDQV RVLFTGKKSQ LAEQSKALGK MDPEQRKVQG 70 80 90 100 110 120 AAIHAVREAI NAALTERQTA LQQAALQQKL ASETIDITLP GRGQHMGSIH PVTQVQERIC 130 140 150 160 170 180 QFFTKAGFSV ATGPEVEDDY HNFEALNIPG HHPARAMHDT FYFDVNHLLR THTSGVQIRT 190 200 210 220 230 240 METTQPPIRI VCPGRVYRCD SDQTHSPMFH QIEGLYVAEN TSFAELKGLL INLLNEFFEK 250 260 270 280 290 300 DLKVRFRPSY FPFTEPSAEV DIMDERGRWL EVLGCGMVHP NVLRAAGIDP EKYKGFAFGL 310 320 330 GVERFAMLRY GINDLRMFYQ NDVRFLRQFA

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ADP1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG69743.1.
RefSeq	YP_047565.1. NC_005966.1.
3D structure databases
ProteinModelPortal	Q6F872.
SMR	Q6F872. Positions 85-330.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6F872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2880188.
GenomeReviews	Gene locus ACIAD3042 in contig CR543861_GR.
KEGG	aci:ACIAD3042.
NMPDR	fig|62977.3.peg.2758.
PATRIC	20743587. VBIAciSp98416_2717.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q6F872.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD3042-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ACIAD

Accession

Primary (citable) accession number: Q6F872

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program