Reviewed, UniProtKB/Swiss-Prot Q6F860 (SYT_ACIAD)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	ACIAD3055

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_0000100930
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F860-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 8F35F4D552E4AF24	FASTA	640	72,996
	10 20 30 40 50 60 MPIITLPNGD QKSFDQPVSV MQVAQSIGPG LAKNTVAGRV NDRLVDACDL ITEDATLQII 70 80 90 100 110 120 TPKDPEGVEI IRHSCAHLVG HAVKQLFPDV QMVIGPVIEE GFYYDIFSPK PFTLDDMAAI 130 140 150 160 170 180 EARMKQLIDQ DYDVVKKMTP REQVIQEFTT RGETYKLRLI DDMPEETQMG LYYHQEYVDM 190 200 210 220 230 240 CRGPHVPNTK FLKNFKLTKI SGAYWRGDAK NEQLQRIYGT AWSDKKELAA YIKRIEEAEK 250 260 270 280 290 300 RDHRKIGKAL DLFHMQEEAP GMVFWHANGW TIYQALEQYM RKVQQDNGYQ EVRTPQIVDF 310 320 330 340 350 360 TLWEKSGHAA NYAENMFTTH SESRNYAVKP MNCPCHVQVF NQGLKSYRDL PVRLAEFGSC 370 380 390 400 410 420 HRNEPSGSLH GIMRVRGFTQ DDGHIFCTKE QIGKEVADFI QLTLDVYKDF GFEDVQMKLS 430 440 450 460 470 480 TRPEKRVGDD RLWDLAEKSL ADALDAAGLE WELQPGEGAF YGPKIEFSLK DCLGRVWQCG 490 500 510 520 530 540 TIQCDFNLPI RLDASFVTED NERDQPVMLH RAILGSFERF IGILIEHYAG FMPPWLAPVQ 550 560 570 580 590 600 ACVMNITDSQ AEACQQVVAK LKENGLRAIS DLRNEKIGFK IRERTLERIP YLLVLGDREV 610 620 630 640 EEGTVNVRTR SGKNLGTMSI DAFVDLVKSA VAERGRYIVE

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG69755.1.
RefSeq	YP_047577.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6F860.
Genome annotation databases
GeneID	2880751.
GenomeReviews	Gene locus ACIAD3055 in contig CR543861_GR.
KEGG	aci:ACIAD3055.
NMPDR	fig|62977.3.peg.2770.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6F860.
OMA	ELELFMF.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD3055-MON.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ACIAD

Accession

Primary (citable) accession number: Q6F860

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)