Reviewed, UniProtKB/Swiss-Prot Q6F7X1 (MDH_ACIAD)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	ACIAD3155

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Malate dehydrogenase		PRO_0000113343
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	131 – 133	3	NAD By similarity
Sites
	Active site	189	1	Proton acceptor By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	107	1	NAD By similarity
	Binding site	114	1	NAD By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F7X1-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 22AE35B28B08B5AB	FASTA	328	35,449
	10 20 30 40 50 60 MKQPVRVAVT GAAGQIGYSL LFRIASGEML GKDQPVILQL LEIPVEKAQQ ALKGVMMELD 70 80 90 100 110 120 DCAFPLLAGM IGTDDPKVAF KDADYALLVG SRPRGPGMER ADLLKVNGEI FIGQGQALNE 130 140 150 160 170 180 VASRDVKVLV VGNPANTNAY IAMKSAPDLP AKNFTAMLRL DHNRALTQIA QKAGVAVADI 190 200 210 220 230 240 EKLTVWGNHS PTMYADYRFA TANGENLKDK INDAEWNKDV FLPTVGKRGA AIIEARGLSS 250 260 270 280 290 300 AASAANAAID HMRDWALGTN GKWVTMGIPS DGSYGIPEGV IYGVPVTCEN GEYKRVEGLE 310 320 IDEFSRERMD KTLQELEEER AAIADMLK

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG69844.1.
RefSeq	YP_047666.1.
3D structure databases
SMR	Q6F7X1. Positions 2-327.
ModBase	Search...
Genome annotation databases
GeneID	2880577.
GenomeReviews	Gene locus ACIAD3155 in contig CR543861_GR.
KEGG	aci:ACIAD3155.
NMPDR	fig|62977.3.peg.2859.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6F7X1.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD3155-MON.
Family and domain databases
HAMAP	MF_01517. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR010945. Malate_DHase_SF1. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_ACIAD

Accession

Primary (citable) accession number: Q6F7X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)