Reviewed, UniProtKB/Swiss-Prot Q6F705 (DDL_ACIAD)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	ACIAD3515

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	D-alanine--D-alanine ligase		PRO_0000341046
Regions
	Domain	104 – 301	198	ATP-grasp
	Nucleotide binding	130 – 185	56	ATP By similarity
Sites
	Metal binding	255	1	Magnesium or manganese 1 By similarity
	Metal binding	268	1	Magnesium or manganese 1 By similarity
	Metal binding	268	1	Magnesium or manganese 2 By similarity
	Metal binding	270	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F705-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 25B74A464E42C3B8	FASTA	309	33,434
	10 20 30 40 50 60 MSNASKFGKV AVLFGGKSAE RDVSLDSGQA VLDALLRSGV QAEAFDPKER SVTELVGYDR 70 80 90 100 110 120 AFIVLHGRGG EDGQIQGVLE WLDIPYTGTG VQGSAIGMDK IKTKQIWQGS DLPTAPYRIV 130 140 150 160 170 180 SKDSDLNEIV ASLGLPVIIK PVHEGSSIGM SKVEKIEDFA PAIEKATAHD AIVMAEKWIT 190 200 210 220 230 240 GREYTIVVLN GKALPVIRLQ PPQDVAFYDY DAKYQRNDVQ YGIPSGLSDS DEKKLQELTL 250 260 270 280 290 300 RAFHTVGASG WGRVDAMQDE HGNFWLLEIN TVPGMTSHSL VPKAAQAIGI DFDALCVEIL AQTLTGLAH

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR543861 Genomic DNA. Translation: CAG70160.1.
RefSeq	YP_047982.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2881096.
GenomeReviews	Gene locus ACIAD3515 in contig CR543861_GR.
KEGG	aci:ACIAD3515.
NMPDR	fig|62977.3.peg.3175.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6F705.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD3515-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ACIAD

Accession

Primary (citable) accession number: Q6F705

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)