Reviewed, UniProtKB/Swiss-Prot Q6F704 (MURC_ACIAD)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	ACIAD3516

Organism

Acinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]

Taxonomic identifier

62977 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182043
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6F704-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 3B98BD440BBC480A	FASTA	482	52,787
	10 20 30 40 50 60 MSPTTPADQA KKLIKVPEMR RIKHIYFVGI GGAGMCGIAE VLKNQGYKVS GSDIKASKTT 70 80 90 100 110 120 AHLEQKGIKV YIGHTADNLK AVDVLVVSTA IDPENPEVKA AIENRIPVVR RAEMLGELMR 130 140 150 160 170 180 YRHGIAVAGT HGKTTTTSLL TTMLAEENMD PTYVIGGLLN RTGVNAALGA SRFIVAEADE 190 200 210 220 230 240 SDASFLYLQP MAAIITNVDA DHMDTYGGSF DKLKDTFVEF IHRLPFYGLA VVCGDDHNVR 250 260 270 280 290 300 EIMPRFGRPT LTYGFNEDND IRAVDVVQEG MQSHFTVLRK DREPLRLTVN QPGLHNILNA 310 320 330 340 350 360 LAAIGVATDE GVSDAAIARA LEGFSGVGRR FQVQGEFELG EGSVKLVDDY GHHPKEVEAT 370 380 390 400 410 420 IKAARQSHPD RRLVMMFQPH RFSRTRDCFD DFVEVLSQVD QLLLLEVYPA GEKPIVGADS 430 440 450 460 470 480 RTLARSIRLR GDVEPILVDP VEGNLPNIIQ KVLQPNDLLL TQGAGNVGAI SVDLAQQQLY LK

References

[1]

"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium." Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C. Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR543861 Genomic DNA. Translation: CAG70161.1.
RefSeq	YP_047983.1.
3D structure databases
SMR	Q6F704. Positions 16-479.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6F704.
Genome annotation databases
GeneID	2879155.
GenomeReviews	Gene locus ACIAD3516 in contig CR543861_GR.
KEGG	aci:ACIAD3516.
NMPDR	fig|62977.3.peg.3176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	Q6F704.
Enzyme and pathway databases
BioCyc	ASP62977:ACIAD3516-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACIAD

Accession

Primary (citable) accession number: Q6F704

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)