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Q6F6Z6 (PYRE_ACIAD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE
Ordered Locus Names:ACIAD3525
OrganismAcinetobacter sp. (strain ADP1) [Complete proteome] [HAMAP]
Taxonomic identifier62977 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Sequence caution

The sequence CAG70169.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMagnesium
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

pyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionorotate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_0000110661

Regions

Region38 – 392Orotate binding By similarity
Region75 – 7625-phosphoribose 1-diphosphate binding By similarity
Region128 – 13695-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site3015-phosphoribose 1-diphosphate By similarity
Binding site10215-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10315-phosphoribose 1-diphosphate By similarity
Binding site10615-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10815-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1321Orotate By similarity
Binding site1601Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6F6Z6 [UniParc].

Last modified August 21, 2007. Version 2.
Checksum: FA9F6067752858EE

FASTA21623,336
        10         20         30         40         50         60 
MTTPSSFNPQ AFIELALSRG VLKFGEFTLK SGRVSPYFFN AGLLNDGEAL SLLAQGYADQ 

        70         80         90        100        110        120 
LMQCQHVDVI FGPAYKGIPF VAATAVALSQ LHAKSVPWGF NRKEAKDHGE GGVLVGASVE 

       130        140        150        160        170        180 
GKKVWIIDDV ITAGTAIREV VTILKNAGAT IAGVLVALDR QERGQGQLSA IQEVQQELEI 

       190        200        210 
PVHALITMKD LMNYLDAKGE TQALAKMEDY RLKYGI

« Hide

References

[1]"Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium."
Barbe V., Vallenet D., Fonknechten N., Kreimeyer A., Oztas S., Labarre L., Cruveiller S., Robert C., Duprat S., Wincker P., Ornston L.N., Weissenbach J., Marliere P., Cohen G.N., Medigue C.
Nucleic Acids Res. 32:5766-5779(2004) [PubMed: 15514110] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ADP1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR543861 Genomic DNA. Translation: CAG70169.1. Different initiation.
RefSeqYP_047991.1. NC_005966.1.

3D structure databases

ProteinModelPortalQ6F6Z6.
SMRQ6F6Z6. Positions 9-216.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6F6Z6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2879566.
GenomeReviewsGene locus ACIAD3525 in contig CR543861_GR.
KEGGaci:ACIAD3525.
NMPDRfig|62977.3.peg.3184.
PATRIC20744481. VBIAciSp98416_3149.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0461.
HOGENOMHBG404341.
ProtClustDBPRK00455.

Enzyme and pathway databases

BioCycASP62977:ACIAD3525-MONOMER.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
KOK00762.
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_ACIAD
AccessionPrimary (citable) accession number: Q6F6Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: August 21, 2007
Last modified: January 25, 2012
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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