Reviewed, UniProtKB/Swiss-Prot Q6F596 (GGPPS_RAT)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl pyrophosphate synthetase
      Short name=GGPP synthetase
      Short name=GGPPSase
Alternative name(s):
    Geranylgeranyl diphosphate synthase
Including the following 3 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10
    3- Recommended name:
            Farnesyltranstransferase
              EC=2.5.1.29

Gene names

Name:

Ggps1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate, an important precursor of carotenoids and geranylated proteins.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Pathway	Isoprenoid biosynthesis; farnesyl-PP biosynthesis; farnesyl-PP from geranyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranyl-PP biosynthesis; geranyl-PP from dimethylallyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranylgeranyl-PP biosynthesis; geranylgeranyl-PP from farnesyl-PP and isopentenyl-PP: step 1/1.
Subunit structure	Homooctamer By similarity.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW soluble fraction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC farnesyltranstransferase activity Inferred from sequence or structural similarity. Source: UniProtKB geranyltranstransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Geranylgeranyl pyrophosphate synthetase		PRO_0000123964

Sequences

	Sequence		Length	Mass (Da)
	Q6F596-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: A3C47E3F9BB81876	FASTA	300	34,778
	10 20 30 40 50 60 MEKTKEKAER ILLEPYKYLL QLPGKQVRTK LSQAFNHWLK VPEDKLQIII EVTEMLHNAS 70 80 90 100 110 120 LLIDDIEDSS KLRRGFPVAH SIYGVPSVIN SANYVYFLGL EKVLTLDHPD AVKLFTRQLL 130 140 150 160 170 180 ELHQGQGLDI YWRDTYTCPT EEEYKAMVLQ KTGGLFGLAV GLMQLFSDYK EDLKPLLDTL 190 200 210 220 230 240 GLFFQIRDDY ANLHSKEYSE NKSFCEDLTE GKFSFPTIHA IWSRPESTQV QNILRQRTEN 250 260 270 280 290 300 IDIKKYCVQY LEDVGSFEYT RYTLRELEAK AYKQIEACGG NPSLVALVKH LSKMFTEENE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Relationship between intron 4b splicing of the rat geranylgeranyl diphosphate synthase gene and the active enzyme expression level." Matsumura Y., Kuzuguchi T., Sagami H. J. Biochem. 136:301-312(2004) [PubMed: 15598886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB118237 Genomic DNA. Translation: BAD30051.1. AB118238 mRNA. Translation: BAD30052.1. AB118239 mRNA. Translation: BAD30053.1. AB118240 mRNA. Translation: BAD30054.1. BC090327 mRNA. Translation: AAH90327.1.
RefSeq	NP_001007627.1.
UniGene	Rn.54975
3D structure databases
SMR	Q6F596. Positions 6-295.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000016767. Rattus norvegicus. [Contig view]
GeneID	291211.
KEGG	rno:291211.
NMPDR	fig|10116.3.peg.13440.
Organism-specific databases
RGD	1359680. Ggps1.
Phylogenomic databases
HOVERGEN	Q6F596.
Gene expression databases
ArrayExpress	Q6F596.
GermOnline	ENSRNOG00000016767. Rattus norvegicus.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	632273.

Entry information

Entry name

GGPPS_RAT

Accession

Primary (citable) accession number: Q6F596

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)