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Protein

Diacetylchitobiose deacetylase

Gene

dac

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deacylates the non-reducing end of diacetylchitobiose (GlcNAc2). Can also use N-acetylglucosamine (GlcNAc) and N-acetylchitotriose (GlcNAc3). Probably involved in chitin degradation.1 Publication

Catalytic activityi

N,N'-diacetylchitobiose + H2O = N-acetyl-beta-D-glucosaminyl-(1->4)-D-glucosamine + acetate.1 Publication

Kineticsi

  1. KM=31.8 mM for GlcNAc2 (at pH 8 and at 70 degrees Celsius)1 Publication
  2. KM=98 mM for GlcNAc3 (at pH 8 and at 70 degrees Celsius)1 Publication
  3. KM=127 mM for GlcNAc (at pH 8 and at 70 degrees Celsius)1 Publication
  1. Vmax=13.3 µmol/min/mg enzyme with GlcNAc3 (at pH 8 and at 70 degrees Celsius)1 Publication
  2. Vmax=48.5 µmol/min/mg enzyme with GlcNAc2 (at pH 8 and at 70 degrees Celsius)1 Publication
  3. Vmax=133 µmol/min/mg enzyme with GlcNAc (at pH 8 and at 70 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 75 degrees Celsius. Activity levels at 37 and 100 degrees Celsius are 20% of that observed at the optimal temperature.1 Publication

Pathwayi: chitin degradation

This protein is involved in the pathway chitin degradation, which is part of Glycan degradation.1 Publication
View all proteins of this organism that are known to be involved in the pathway chitin degradation and in Glycan degradation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16776
BRENDAi3.5.1.105 5246
3.5.1.33 5246
SABIO-RKiQ6F4N1
UniPathwayiUPA00349

Names & Taxonomyi

Protein namesi
Recommended name:
Diacetylchitobiose deacetylase (EC:3.5.1.1051 Publication)
Alternative name(s):
N-acetylchitobiose deacetylase
Tk-Dac
Gene namesi
Name:dac
Ordered Locus Names:TK1764
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003865481 – 267Diacetylchitobiose deacetylaseAdd BLAST267

Expressioni

Inductioni

By diacetylchitobiose (GlcNAc2).1 Publication

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

IntActiQ6F4N1, 1 interactor
MINTiQ6F4N1
STRINGi69014.TK1764

Structurei

3D structure databases

ProteinModelPortaliQ6F4N1
SMRiQ6F4N1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGL family.Curated

Phylogenomic databases

eggNOGiarCOG03460 Archaea
COG2120 LUCA
HOGENOMiHOG000241170
InParanoidiQ6F4N1
KOiK22408
OMAiEIVLCNA
OrthoDBiPOG093Z0ATI

Family and domain databases

Gene3Di3.40.50.10320, 1 hit
InterProiView protein in InterPro
IPR003737 GlcNAc_PI_deacetylase-related
IPR024078 LmbE-like_dom_sf
PANTHERiPTHR12993 PTHR12993, 1 hit
PfamiView protein in Pfam
PF02585 PIG-L, 1 hit
SUPFAMiSSF102588 SSF102588, 1 hit

Sequencei

Sequence statusi: Complete.

Q6F4N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFEEFNNFD EAFSALLSKL DFKINEPFND VKKVLCIEPH PDDCAIGLGG
60 70 80 90 100
TIKKLTDSGI DVVYLLLTDG SMGTTDGEVS GHELALRRLE EEKRSAEILG
110 120 130 140 150
VKKIHALDFG DTELPYTREV RKEIVTVIRK ERPGIVLMPD PWLPYEGHPD
160 170 180 190 200
HRHAGFLGIE AVSFAGLPNF NRSDLIAGLD PHSIQAVGFY YTHKPNYFVD
210 220 230 240 250
ISDVMEVKLR AVRTHESQFP EDVWELWEPY LRTIALYYGK MSGHRYAEGI
260
RFVPGIFLHI CPFAHVI
Length:267
Mass (Da):30,302
Last modified:August 16, 2004 - v1
Checksum:iEE7740F826B43CD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125969 Genomic DNA Translation: BAD29713.1
AP006878 Genomic DNA Translation: BAD85953.1

Genome annotation databases

EnsemblBacteriaiBAD85953; BAD85953; TK1764
KEGGitko:TK1764
PATRICifig|69014.16.peg.1720

Similar proteinsi

Entry informationi

Entry nameiDCHI_THEKO
AccessioniPrimary (citable) accession number: Q6F4N1
Secondary accession number(s): Q5JDT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: August 16, 2004
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health