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Protein

Diacetylchitobiose deacetylase

Gene

dac

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Deacylates the non-reducing end of diacetylchitobiose (GlcNAc2). Can also use N-acetylglucosamine (GlcNAc) and N-acetylchitotriose (GlcNAc3). Probably involved in chitin degradation.1 Publication

Catalytic activityi

N,N'-diacetylchitobiose + H2O = N-acetyl-beta-D-glucosaminyl-(1->4)-D-glucosamine + acetate.1 Publication

Kineticsi

  1. KM=31.8 mM for GlcNAc2 (at pH 8 and at 70 degrees Celsius)1 Publication
  2. KM=98 mM for GlcNAc3 (at pH 8 and at 70 degrees Celsius)1 Publication
  3. KM=127 mM for GlcNAc (at pH 8 and at 70 degrees Celsius)1 Publication
  1. Vmax=13.3 µmol/min/mg enzyme with GlcNAc3 (at pH 8 and at 70 degrees Celsius)1 Publication
  2. Vmax=48.5 µmol/min/mg enzyme with GlcNAc2 (at pH 8 and at 70 degrees Celsius)1 Publication
  3. Vmax=133 µmol/min/mg enzyme with GlcNAc (at pH 8 and at 70 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 75 degrees Celsius. Activity levels at 37 and 100 degrees Celsius are 20% of that observed at the optimal temperature.1 Publication

Pathwayi: chitin degradation

This protein is involved in the pathway chitin degradation, which is part of Glycan degradation.1 Publication
View all proteins of this organism that are known to be involved in the pathway chitin degradation and in Glycan degradation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16776.
TKOD69014:GH72-1800-MONOMER.
BRENDAi3.5.1.105. 5246.
3.5.1.33. 5246.
SABIO-RKQ6F4N1.
UniPathwayiUPA00349.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacetylchitobiose deacetylase (EC:3.5.1.1051 Publication)
Alternative name(s):
N-acetylchitobiose deacetylase
Tk-Dac
Gene namesi
Name:dac
Ordered Locus Names:TK1764
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Diacetylchitobiose deacetylasePRO_0000386548Add
BLAST

Expressioni

Inductioni

By diacetylchitobiose (GlcNAc2).1 Publication

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

IntActiQ6F4N1. 1 interaction.
MINTiMINT-8377160.
STRINGi69014.TK1764.

Structurei

3D structure databases

ProteinModelPortaliQ6F4N1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGL family.Curated

Phylogenomic databases

eggNOGiarCOG03460. Archaea.
COG2120. LUCA.
HOGENOMiHOG000241170.
InParanoidiQ6F4N1.
OMAiCIEPHPD.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6F4N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFEEFNNFD EAFSALLSKL DFKINEPFND VKKVLCIEPH PDDCAIGLGG
60 70 80 90 100
TIKKLTDSGI DVVYLLLTDG SMGTTDGEVS GHELALRRLE EEKRSAEILG
110 120 130 140 150
VKKIHALDFG DTELPYTREV RKEIVTVIRK ERPGIVLMPD PWLPYEGHPD
160 170 180 190 200
HRHAGFLGIE AVSFAGLPNF NRSDLIAGLD PHSIQAVGFY YTHKPNYFVD
210 220 230 240 250
ISDVMEVKLR AVRTHESQFP EDVWELWEPY LRTIALYYGK MSGHRYAEGI
260
RFVPGIFLHI CPFAHVI
Length:267
Mass (Da):30,302
Last modified:August 16, 2004 - v1
Checksum:iEE7740F826B43CD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125969 Genomic DNA. Translation: BAD29713.1.
AP006878 Genomic DNA. Translation: BAD85953.1.

Genome annotation databases

EnsemblBacteriaiBAD85953; BAD85953; TK1764.
KEGGitko:TK1764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB125969 Genomic DNA. Translation: BAD29713.1.
AP006878 Genomic DNA. Translation: BAD85953.1.

3D structure databases

ProteinModelPortaliQ6F4N1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6F4N1. 1 interaction.
MINTiMINT-8377160.
STRINGi69014.TK1764.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD85953; BAD85953; TK1764.
KEGGitko:TK1764.

Phylogenomic databases

eggNOGiarCOG03460. Archaea.
COG2120. LUCA.
HOGENOMiHOG000241170.
InParanoidiQ6F4N1.
OMAiCIEPHPD.

Enzyme and pathway databases

UniPathwayiUPA00349.
BioCyciMetaCyc:MONOMER-16776.
TKOD69014:GH72-1800-MONOMER.
BRENDAi3.5.1.105. 5246.
3.5.1.33. 5246.
SABIO-RKQ6F4N1.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCHI_THEKO
AccessioniPrimary (citable) accession number: Q6F4N1
Secondary accession number(s): Q5JDT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: August 16, 2004
Last modified: November 11, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.