SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q6F494

- PCKG_THEKO

UniProt

Q6F494 - PCKG_THEKO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
pckG, pck, TK1405
Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.1 Publication

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.1 Publication

Cofactori

Binds 1 manganese ion per subunit.1 Publication

Kineticsi

  1. KM=18.1 µM for oxaloacetate (at 60 degrees Celsius)1 Publication
  2. KM=131 µM for phosphoenolpyruvate (at 60 degrees Celsius)
  3. KM=18.5 µM for GDP (at 60 degrees Celsius)
  4. KM=36.1 µM for GTP (at 60 degrees Celsius)

Vmax=44.4 µmol/min/mg enzyme for the forward reaction (at 60 degrees Celsius)

Vmax=76.9 µmol/min/mg enzyme for the reverse reaction (at 60 degrees Celsius)

pH dependencei

Optimum pH is 7.

Temperature dependencei

Optimum temperature is 80 degrees Celsius. Thermostable. Half-life at the optimal temperature is 53 minutes.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861Substrate By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Metal bindingi229 – 2291Manganese By similarity
Binding sitei229 – 2291Substrate By similarity
Metal bindingi248 – 2481Manganese; via tele nitrogen By similarity
Binding sitei270 – 2701Substrate By similarity
Active sitei272 – 2721 By similarity
Metal bindingi289 – 2891Manganese By similarity
Binding sitei386 – 3861GTP By similarity
Binding sitei418 – 4181GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:GH72-1426-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Alternative name(s):
PckTk
Gene namesi
Name:pckG
Synonyms:pck
Ordered Locus Names:TK1405
OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000000536: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Phosphoenolpyruvate carboxykinase [GTP]UniRule annotationPRO_0000103620Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi69014.TK1405.

Structurei

3D structure databases

ProteinModelPortaliQ6F494.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 3863Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiIACNIGR.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6F494-1 [UniParc]FASTAAdd to Basket

« Hide

MNALERLEKL LDKEQFEKVK AIDNPELHEF LAEWIEWLEP DKVFVCTDSP    50
EDEGYVRWKA LYYGEERMLE TPNHTVHYDN YYDQARDKAN TAILLPGGKK 100
LPYINTKDRD EGLKEIRELM KGIMKGKELF VCFFVLGPKN SIFTIPAVQL 150
TDSAYVAHSE FILYRKGYEE FKRLGRSARF FRFVHSAGEL DERKTSKNLD 200
KRRIYIDLED ETVYSVNTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF 250
LMRVNGPHGR KTYFTGAYPS MCGKTSTAMI PWENIVGDDL TFILPVNGIA 300
RGANVEKGVF GIIQGVNPED DPIIWQVLHS PVEIIFSNVL VKDGKPYWND 350
MGIEIPDEGE NHSGKWWRGK KDAEGNEIPP SHKNARFTVS LEHFPNVDME 400
ALENPCGVEV GGMIFGGRDA DTWPPVREAF NWEHGVITMG ASLESETTAA 450
TLGKEGVRAF NPMAILDFMS VHLGDYLRNY LEFGRKLKKT PKIFAVNYFL 500
RENGVWLNHK LDKAVWLKWM ELRVHGDVEA IETPIGYIPK YKDLAKLFKD 550
VLNKEYTKED YERQFKIRVP ELLAKIDRIE EIYRKLDNVP EELFKVLEEE 600
RQRLLEAREK YGDYISPFAL EGE 623
Length:623
Mass (Da):72,039
Last modified:August 16, 2004 - v1
Checksum:i91B376391DC8B54E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB167819 Genomic DNA. Translation: BAD26729.1.
AP006878 Genomic DNA. Translation: BAD85594.1.
RefSeqiWP_011250356.1. NC_006624.1.
YP_183818.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85594; BAD85594; TK1405.
GeneIDi3234722.
KEGGitko:TK1405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB167819 Genomic DNA. Translation: BAD26729.1 .
AP006878 Genomic DNA. Translation: BAD85594.1 .
RefSeqi WP_011250356.1. NC_006624.1.
YP_183818.1. NC_006624.1.

3D structure databases

ProteinModelPortali Q6F494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 69014.TK1405.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD85594 ; BAD85594 ; TK1405 .
GeneIDi 3234722.
KEGGi tko:TK1405.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi IACNIGR.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci TKOD69014:GH72-1426-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "First characterization of an archaeal GTP-dependent phosphoenolpyruvate carboxykinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1."
    Fukuda W., Fukui T., Atomi H., Imanaka T.
    J. Bacteriol. 186:4620-4627(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.
  2. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
    Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
    Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-918 / JCM 12380 / KOD1.

Entry informationi

Entry nameiPCKG_THEKO
AccessioniPrimary (citable) accession number: Q6F494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 16, 2004
Last modified: September 3, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi