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Q6F494

- PCKG_THEKO

UniProt

Q6F494 - PCKG_THEKO

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.1 Publication

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.1 Publication

    Cofactori

    Binds 1 manganese ion per subunit.1 Publication

    Kineticsi

    1. KM=18.1 µM for oxaloacetate (at 60 degrees Celsius)1 Publication
    2. KM=131 µM for phosphoenolpyruvate (at 60 degrees Celsius)1 Publication
    3. KM=18.5 µM for GDP (at 60 degrees Celsius)1 Publication
    4. KM=36.1 µM for GTP (at 60 degrees Celsius)1 Publication

    Vmax=44.4 µmol/min/mg enzyme for the forward reaction (at 60 degrees Celsius)1 Publication

    Vmax=76.9 µmol/min/mg enzyme for the reverse reaction (at 60 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.1 Publication

    Temperature dependencei

    Optimum temperature is 80 degrees Celsius. Thermostable. Half-life at the optimal temperature is 53 minutes.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei86 – 861SubstrateBy similarity
    Binding sitei222 – 2221Substrate; via amide nitrogenBy similarity
    Metal bindingi229 – 2291ManganeseBy similarity
    Binding sitei229 – 2291SubstrateBy similarity
    Metal bindingi248 – 2481Manganese; via tele nitrogenBy similarity
    Binding sitei270 – 2701SubstrateBy similarity
    Active sitei272 – 2721By similarity
    Metal bindingi289 – 2891ManganeseBy similarity
    Binding sitei386 – 3861GTPBy similarity
    Binding sitei418 – 4181GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTKOD69014:GH72-1426-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
    Short name:
    PEP carboxykinase
    Short name:
    PEPCK
    Alternative name(s):
    PckTk
    Gene namesi
    Name:pckG
    Synonyms:pck
    Ordered Locus Names:TK1405
    OrganismiThermococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
    Taxonomic identifieri69014 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000000536: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 623623Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103620Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi69014.TK1405.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6F494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni384 – 3863Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiIACNIGR.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6F494-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNALERLEKL LDKEQFEKVK AIDNPELHEF LAEWIEWLEP DKVFVCTDSP    50
    EDEGYVRWKA LYYGEERMLE TPNHTVHYDN YYDQARDKAN TAILLPGGKK 100
    LPYINTKDRD EGLKEIRELM KGIMKGKELF VCFFVLGPKN SIFTIPAVQL 150
    TDSAYVAHSE FILYRKGYEE FKRLGRSARF FRFVHSAGEL DERKTSKNLD 200
    KRRIYIDLED ETVYSVNTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF 250
    LMRVNGPHGR KTYFTGAYPS MCGKTSTAMI PWENIVGDDL TFILPVNGIA 300
    RGANVEKGVF GIIQGVNPED DPIIWQVLHS PVEIIFSNVL VKDGKPYWND 350
    MGIEIPDEGE NHSGKWWRGK KDAEGNEIPP SHKNARFTVS LEHFPNVDME 400
    ALENPCGVEV GGMIFGGRDA DTWPPVREAF NWEHGVITMG ASLESETTAA 450
    TLGKEGVRAF NPMAILDFMS VHLGDYLRNY LEFGRKLKKT PKIFAVNYFL 500
    RENGVWLNHK LDKAVWLKWM ELRVHGDVEA IETPIGYIPK YKDLAKLFKD 550
    VLNKEYTKED YERQFKIRVP ELLAKIDRIE EIYRKLDNVP EELFKVLEEE 600
    RQRLLEAREK YGDYISPFAL EGE 623
    Length:623
    Mass (Da):72,039
    Last modified:August 16, 2004 - v1
    Checksum:i91B376391DC8B54E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB167819 Genomic DNA. Translation: BAD26729.1.
    AP006878 Genomic DNA. Translation: BAD85594.1.
    RefSeqiWP_011250356.1. NC_006624.1.
    YP_183818.1. NC_006624.1.

    Genome annotation databases

    EnsemblBacteriaiBAD85594; BAD85594; TK1405.
    GeneIDi3234722.
    KEGGitko:TK1405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB167819 Genomic DNA. Translation: BAD26729.1 .
    AP006878 Genomic DNA. Translation: BAD85594.1 .
    RefSeqi WP_011250356.1. NC_006624.1.
    YP_183818.1. NC_006624.1.

    3D structure databases

    ProteinModelPortali Q6F494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 69014.TK1405.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD85594 ; BAD85594 ; TK1405 .
    GeneIDi 3234722.
    KEGGi tko:TK1405.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi IACNIGR.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci TKOD69014:GH72-1426-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "First characterization of an archaeal GTP-dependent phosphoenolpyruvate carboxykinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1."
      Fukuda W., Fukui T., Atomi H., Imanaka T.
      J. Bacteriol. 186:4620-4627(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.
    2. "Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes."
      Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.
      Genome Res. 15:352-363(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-918 / JCM 12380 / KOD1.

    Entry informationi

    Entry nameiPCKG_THEKO
    AccessioniPrimary (citable) accession number: Q6F494
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3