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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation1 Publication

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation1 Publication

Cofactori

Mn2+UniRule annotation1 PublicationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation1 Publication

Kineticsi

  1. KM=18.1 µM for oxaloacetate (at 60 degrees Celsius)1 Publication
  2. KM=131 µM for phosphoenolpyruvate (at 60 degrees Celsius)1 Publication
  3. KM=18.5 µM for GDP (at 60 degrees Celsius)1 Publication
  4. KM=36.1 µM for GTP (at 60 degrees Celsius)1 Publication
  1. Vmax=44.4 µmol/min/mg enzyme for the forward reaction (at 60 degrees Celsius)1 Publication
  2. Vmax=76.9 µmol/min/mg enzyme for the reverse reaction (at 60 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Temperature dependencei

Optimum temperature is 80 degrees Celsius. Thermostable. Half-life at the optimal temperature is 53 minutes.1 Publication

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotationCurated
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi248Manganese; via tele nitrogenUniRule annotation1
Binding sitei270SubstrateUniRule annotation1
Active sitei272UniRule annotation1
Metal bindingi289ManganeseUniRule annotation1
Binding sitei386GTPUniRule annotation1
Binding sitei418GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 276GTPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.1.1.32. 5246.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]1 PublicationUniRule annotation (EC:4.1.1.32UniRule annotation1 Publication)
Short name:
PEP carboxykinase1 PublicationUniRule annotation
Short name:
PEPCK1 PublicationUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinase1 PublicationUniRule annotation
Short name:
GTP-PEPCK1 PublicationUniRule annotation
PckTk1 PublicationUniRule annotation
Gene namesi
Name:pckG
Synonyms:pck
Ordered Locus Names:TK1405
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationCurated

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036201 – 623Phosphoenolpyruvate carboxykinase [GTP]Add BLAST623

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi69014.TK1405.

Structurei

3D structure databases

ProteinModelPortaliQ6F494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni384 – 386Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotationCurated

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
HOGENOMiHOG000191700.
InParanoidiQ6F494.
KOiK01596.
OMAiYGENMRV.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6F494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALERLEKL LDKEQFEKVK AIDNPELHEF LAEWIEWLEP DKVFVCTDSP
60 70 80 90 100
EDEGYVRWKA LYYGEERMLE TPNHTVHYDN YYDQARDKAN TAILLPGGKK
110 120 130 140 150
LPYINTKDRD EGLKEIRELM KGIMKGKELF VCFFVLGPKN SIFTIPAVQL
160 170 180 190 200
TDSAYVAHSE FILYRKGYEE FKRLGRSARF FRFVHSAGEL DERKTSKNLD
210 220 230 240 250
KRRIYIDLED ETVYSVNTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF
260 270 280 290 300
LMRVNGPHGR KTYFTGAYPS MCGKTSTAMI PWENIVGDDL TFILPVNGIA
310 320 330 340 350
RGANVEKGVF GIIQGVNPED DPIIWQVLHS PVEIIFSNVL VKDGKPYWND
360 370 380 390 400
MGIEIPDEGE NHSGKWWRGK KDAEGNEIPP SHKNARFTVS LEHFPNVDME
410 420 430 440 450
ALENPCGVEV GGMIFGGRDA DTWPPVREAF NWEHGVITMG ASLESETTAA
460 470 480 490 500
TLGKEGVRAF NPMAILDFMS VHLGDYLRNY LEFGRKLKKT PKIFAVNYFL
510 520 530 540 550
RENGVWLNHK LDKAVWLKWM ELRVHGDVEA IETPIGYIPK YKDLAKLFKD
560 570 580 590 600
VLNKEYTKED YERQFKIRVP ELLAKIDRIE EIYRKLDNVP EELFKVLEEE
610 620
RQRLLEAREK YGDYISPFAL EGE
Length:623
Mass (Da):72,039
Last modified:August 16, 2004 - v1
Checksum:i91B376391DC8B54E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB167819 Genomic DNA. Translation: BAD26729.1.
AP006878 Genomic DNA. Translation: BAD85594.1.
RefSeqiWP_011250356.1. NC_006624.1.

Genome annotation databases

EnsemblBacteriaiBAD85594; BAD85594; TK1405.
GeneIDi3234722.
KEGGitko:TK1405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB167819 Genomic DNA. Translation: BAD26729.1.
AP006878 Genomic DNA. Translation: BAD85594.1.
RefSeqiWP_011250356.1. NC_006624.1.

3D structure databases

ProteinModelPortaliQ6F494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi69014.TK1405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD85594; BAD85594; TK1405.
GeneIDi3234722.
KEGGitko:TK1405.

Phylogenomic databases

eggNOGiarCOG05865. Archaea.
COG1274. LUCA.
HOGENOMiHOG000191700.
InParanoidiQ6F494.
KOiK01596.
OMAiYGENMRV.

Enzyme and pathway databases

UniPathwayiUPA00138.
BRENDAi4.1.1.32. 5246.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_THEKO
AccessioniPrimary (citable) accession number: Q6F494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.