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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation1 Publication

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation1 Publication

Cofactori

Mn2+UniRule annotation1 PublicationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation1 Publication

Kineticsi

  1. KM=18.1 µM for oxaloacetate (at 60 degrees Celsius)1 Publication
  2. KM=131 µM for phosphoenolpyruvate (at 60 degrees Celsius)1 Publication
  3. KM=18.5 µM for GDP (at 60 degrees Celsius)1 Publication
  4. KM=36.1 µM for GTP (at 60 degrees Celsius)1 Publication
  1. Vmax=44.4 µmol/min/mg enzyme for the forward reaction (at 60 degrees Celsius)1 Publication
  2. Vmax=76.9 µmol/min/mg enzyme for the reverse reaction (at 60 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Temperature dependencei

Optimum temperature is 80 degrees Celsius. Thermostable. Half-life at the optimal temperature is 53 minutes.1 Publication

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotationCurated
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi248Manganese; via tele nitrogenUniRule annotation1
Binding sitei270SubstrateUniRule annotation1
Active sitei272UniRule annotation1
Metal bindingi289ManganeseUniRule annotation1
Binding sitei386GTPUniRule annotation1
Binding sitei418GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 276GTPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTKOD69014:G1G2A-1401-MONOMER
BRENDAi4.1.1.32 5246
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]1 PublicationUniRule annotation (EC:4.1.1.32UniRule annotation1 Publication)
Short name:
PEP carboxykinase1 PublicationUniRule annotation
Short name:
PEPCK1 PublicationUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinase1 PublicationUniRule annotation
Short name:
GTP-PEPCK1 PublicationUniRule annotation
PckTk1 PublicationUniRule annotation
Gene namesi
Name:pckG
Synonyms:pck
Ordered Locus Names:TK1405
OrganismiThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Taxonomic identifieri69014 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000000536 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationCurated

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036201 – 623Phosphoenolpyruvate carboxykinase [GTP]Add BLAST623

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi69014.TK1405

Structurei

3D structure databases

ProteinModelPortaliQ6F494
SMRiQ6F494
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni384 – 386Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotationCurated

Phylogenomic databases

eggNOGiarCOG05865 Archaea
COG1274 LUCA
HOGENOMiHOG000191700
InParanoidiQ6F494
KOiK01596
OMAiCWLALTD
OrthoDBiPOG093Z01SN

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q6F494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALERLEKL LDKEQFEKVK AIDNPELHEF LAEWIEWLEP DKVFVCTDSP
60 70 80 90 100
EDEGYVRWKA LYYGEERMLE TPNHTVHYDN YYDQARDKAN TAILLPGGKK
110 120 130 140 150
LPYINTKDRD EGLKEIRELM KGIMKGKELF VCFFVLGPKN SIFTIPAVQL
160 170 180 190 200
TDSAYVAHSE FILYRKGYEE FKRLGRSARF FRFVHSAGEL DERKTSKNLD
210 220 230 240 250
KRRIYIDLED ETVYSVNTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF
260 270 280 290 300
LMRVNGPHGR KTYFTGAYPS MCGKTSTAMI PWENIVGDDL TFILPVNGIA
310 320 330 340 350
RGANVEKGVF GIIQGVNPED DPIIWQVLHS PVEIIFSNVL VKDGKPYWND
360 370 380 390 400
MGIEIPDEGE NHSGKWWRGK KDAEGNEIPP SHKNARFTVS LEHFPNVDME
410 420 430 440 450
ALENPCGVEV GGMIFGGRDA DTWPPVREAF NWEHGVITMG ASLESETTAA
460 470 480 490 500
TLGKEGVRAF NPMAILDFMS VHLGDYLRNY LEFGRKLKKT PKIFAVNYFL
510 520 530 540 550
RENGVWLNHK LDKAVWLKWM ELRVHGDVEA IETPIGYIPK YKDLAKLFKD
560 570 580 590 600
VLNKEYTKED YERQFKIRVP ELLAKIDRIE EIYRKLDNVP EELFKVLEEE
610 620
RQRLLEAREK YGDYISPFAL EGE
Length:623
Mass (Da):72,039
Last modified:August 16, 2004 - v1
Checksum:i91B376391DC8B54E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB167819 Genomic DNA Translation: BAD26729.1
AP006878 Genomic DNA Translation: BAD85594.1
RefSeqiWP_011250356.1, NC_006624.1

Genome annotation databases

EnsemblBacteriaiBAD85594; BAD85594; TK1405
GeneIDi3234722
KEGGitko:TK1405
PATRICifig|69014.16.peg.1367

Similar proteinsi

Entry informationi

Entry nameiPCKG_THEKO
AccessioniPrimary (citable) accession number: Q6F494
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: August 16, 2004
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health