Q6F1V4 (GATB_MESFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	Mfl163

Organism

Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum) [Reference proteome] [HAMAP]

Taxonomic identifier

265311 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Entomoplasmataceae › Mesoplasma › Mesoplasma florum

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_0000241237

Sequences

	Sequence		Length	Mass (Da)
	Q6F1V4 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: B24DFA899127259B	FASTA	479	54,705
	10 20 30 40 50 60 MRNFEIVIGI ENHVELKTKS KMFSSAPVSY GETPNTNVNE TDMAYPGSLP TINKKGIELA 70 80 90 100 110 120 IRTCNALNME IDTLVKFDRK NYFYPDLTKG YQITQQYNPI GKNGKLNINV NGLTKEVDIE 130 140 150 160 170 180 RLHMEEDTAK QIHKDDLTYI DYNRAGTGLV EIVTRPVLRS ADEACAYVEK LREVLLFLKV 190 200 210 220 230 240 SDVKMNEGSL RTDVNISIRP FGTQEFSNKV EVKNLNSISN IKKAIEFEVE RQTKLMLNNE 250 260 270 280 290 300 IIIQETRRFD DTTNSTVSMR SKSDALDYKY FREPNIMPIQ LKKEWVEDCI KNSPELADIK 310 320 330 340 350 360 RIKYVNDYKI SINDANIILT SIEMTEFFEE TIKFTNNYTK VANILISDIQ AQLNNENTTI 370 380 390 400 410 420 DKLALLPSHL AEMINLVDQS VISSKHTKTI LPIIMKDSSK SVIEIVEELN IKMISDENEI 430 440 450 460 470 ANLVNPIIES NLELLEQYSE RPERVTKTIM GQLMKVTGGN VNPEAGMNII IKLVENKIK

References

[1]

Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017263 Genomic DNA. Translation: AAT75519.1.
RefSeq	YP_053403.1. NC_006055.1.
3D structure databases
ProteinModelPortal	Q6F1V4.
ModBase	Search...
Protein-protein interaction databases
STRING	265311.Mfl163.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT75519; AAT75519; Mfl163.
GeneID	2897853.
KEGG	mfl:Mfl163.
PATRIC	22473035. VBIMesFlo3168_0164.
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223742.
KO	K02434.
OMA	GWVEIEV.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	MFLO265311:GHIB-184-MONOMER.
Family and domain databases
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_MESFL

Accession

Primary (citable) accession number: Q6F1V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program