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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Asp/Glu-ADT subunit BUniRule annotation
Gene namesi
Name:gatBUniRule annotation
Ordered Locus Names:Mfl163
OrganismiMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Taxonomic identifieri265311 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma
Proteomesi
  • UP000006647 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002412371 – 479Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BAdd BLAST479

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi265311.Mfl163.

Structurei

3D structure databases

ProteinModelPortaliQ6F1V4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
OrthoDBiPOG091H01A7.

Family and domain databases

HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6F1V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNFEIVIGI ENHVELKTKS KMFSSAPVSY GETPNTNVNE TDMAYPGSLP
60 70 80 90 100
TINKKGIELA IRTCNALNME IDTLVKFDRK NYFYPDLTKG YQITQQYNPI
110 120 130 140 150
GKNGKLNINV NGLTKEVDIE RLHMEEDTAK QIHKDDLTYI DYNRAGTGLV
160 170 180 190 200
EIVTRPVLRS ADEACAYVEK LREVLLFLKV SDVKMNEGSL RTDVNISIRP
210 220 230 240 250
FGTQEFSNKV EVKNLNSISN IKKAIEFEVE RQTKLMLNNE IIIQETRRFD
260 270 280 290 300
DTTNSTVSMR SKSDALDYKY FREPNIMPIQ LKKEWVEDCI KNSPELADIK
310 320 330 340 350
RIKYVNDYKI SINDANIILT SIEMTEFFEE TIKFTNNYTK VANILISDIQ
360 370 380 390 400
AQLNNENTTI DKLALLPSHL AEMINLVDQS VISSKHTKTI LPIIMKDSSK
410 420 430 440 450
SVIEIVEELN IKMISDENEI ANLVNPIIES NLELLEQYSE RPERVTKTIM
460 470
GQLMKVTGGN VNPEAGMNII IKLVENKIK
Length:479
Mass (Da):54,705
Last modified:August 16, 2004 - v1
Checksum:iB24DFA899127259B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75519.1.
RefSeqiWP_011183060.1. NC_006055.1.
YP_053403.1. NC_006055.1.

Genome annotation databases

EnsemblBacteriaiAAT75519; AAT75519; Mfl163.
GeneIDi2897853.
KEGGimfl:Mfl163.
PATRICi22473035. VBIMesFlo3168_0164.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75519.1.
RefSeqiWP_011183060.1. NC_006055.1.
YP_053403.1. NC_006055.1.

3D structure databases

ProteinModelPortaliQ6F1V4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi265311.Mfl163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT75519; AAT75519; Mfl163.
GeneIDi2897853.
KEGGimfl:Mfl163.
PATRICi22473035. VBIMesFlo3168_0164.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
OrthoDBiPOG091H01A7.

Family and domain databases

HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATB_MESFL
AccessioniPrimary (citable) accession number: Q6F1V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.