Reviewed, UniProtKB/Swiss-Prot Q6F1U6 (GUAC_MESFL)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	Mfl170

Organism

Mesoplasma florum (Acholeplasma florum) [Complete proteome] [HAMAP]

Taxonomic identifier

2151 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Entomoplasmataceae › Mesoplasma

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	GMP reductase HAMAP MF_01511		PRO_0000294277
Regions
	Nucleotide binding	203 – 226	24	NADP Potential
Sites
	Active site	174	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F1U6-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 53933FE2937D8CCD	FASTA	320	35,585
	10 20 30 40 50 60 MYAFDYEDIQ LIPNMCVVNS RSECNTSVTL GKHTFKMPVV PANMATVINE ELSIMLAEKN 70 80 90 100 110 120 YFYVMHRFDF DAVSFIKKMK EKKLISSISV GVKEQDFKMI NELTELNLIP DYITIDIAHG 130 140 150 160 170 180 HANSVKEMIE HIRTKMGDQT FIIAGNVATP QAVRDLEHWG ADATKVGVGP GKVCITKLKT 190 200 210 220 230 240 GFGTGGWQLG AIKWCSKAAT KPIIADGGLR VNGDIAKSIR FGATMCMIGS LFAAHEESPG 250 260 270 280 290 300 KNVTVDNVLF KEYYGSASEY NKGEKRYVEG KKELIKVRGK LMDTYKEMEE DLQSSISYAG 310 320 GKTLKAIKKV DYVILKTSNF

References

[1]

Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33453 / L1 / NBRC 100688 / NCTC 11704.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017263 Genomic DNA. Translation: AAT75527.1.
RefSeq	YP_053411.1.
3D structure databases
HSSP	HSSP built from PDB template 1YPF based on UniProtKB Q81JJ9.
SMR	Q6F1U6. Positions 4-320.
ModBase	Search...
Genome annotation databases
GeneID	2898130.
GenomeReviews	Gene locus Mfl170 in contig AE017263_GR.
KEGG	mfl:Mfl170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FAGHEES.
Enzyme and pathway databases
BioCyc	MFLO265311:MFL170-MONOMER.
BRENDA	1.7.1.7. 281333.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_MESFL

Accession

Primary (citable) accession number: Q6F1U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)