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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451S-adenosyl-L-methionineUniRule annotation
Binding sitei70 – 701S-adenosyl-L-methionineUniRule annotation
Active sitei125 – 1251By similarity
Binding sitei125 – 1251S-adenosyl-L-methionineUniRule annotation
Binding sitei129 – 1291SubstrateUniRule annotation
Binding sitei161 – 1611SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMFLO265311:GHIB-239-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Mfl218
OrganismiMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Taxonomic identifieri265311 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma
ProteomesiUP000006647: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223tRNA (guanine-N(7)-)-methyltransferasePRO_0000171347Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi265311.Mfl218.

Structurei

3D structure databases

ProteinModelPortaliQ6F1P8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 2044Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiRLINTEF.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6F1P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNKPWVK EYLEKNDKYL ISWDKETKIN LSDLFNNKKQ PVHLEIGCGK
60 70 80 90 100
GNFITNHALK ESDINFIGME KEETVVGVAL KKTLAEFEQR NKEVTNLKYF
110 120 130 140 150
NDFAEDLSDI FAPSSIDKIY LNFSDPWPKA RHSKKRLTYR TFLDIYANII
160 170 180 190 200
KSHGILEFKT DNDGLFAFSL EEIAENKNWE LIYQTTDLYS DIEALKNNIP
210 220
TEYETKFHTA GKNINKLIIK KTF
Length:223
Mass (Da):26,158
Last modified:August 16, 2004 - v1
Checksum:i719328A9762616DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75575.1.
RefSeqiYP_053459.1. NC_006055.1.

Genome annotation databases

EnsemblBacteriaiAAT75575; AAT75575; Mfl218.
GeneIDi2898269.
KEGGimfl:Mfl218.
PATRICi22473161. VBIMesFlo3168_0218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75575.1.
RefSeqiYP_053459.1. NC_006055.1.

3D structure databases

ProteinModelPortaliQ6F1P8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi265311.Mfl218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT75575; AAT75575; Mfl218.
GeneIDi2898269.
KEGGimfl:Mfl218.
PATRICi22473161. VBIMesFlo3168_0218.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiRLINTEF.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciMFLO265311:GHIB-239-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Entry informationi

Entry nameiTRMB_MESFL
AccessioniPrimary (citable) accession number: Q6F1P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 16, 2004
Last modified: March 4, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.