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Q6F1P8 (TRMB_MESFL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Mfl218
OrganismMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum) [Reference proteome] [HAMAP]
Taxonomic identifier265311 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 223223tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171347

Regions

Region201 – 2044Substrate binding By similarity

Sites

Active site1251 By similarity
Binding site451S-adenosyl-L-methionine By similarity
Binding site701S-adenosyl-L-methionine By similarity
Binding site1251S-adenosyl-L-methionine By similarity
Binding site1291Substrate By similarity
Binding site1611Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6F1P8 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 719328A9762616DA

FASTA22326,158
        10         20         30         40         50         60 
MRLRNKPWVK EYLEKNDKYL ISWDKETKIN LSDLFNNKKQ PVHLEIGCGK GNFITNHALK 

        70         80         90        100        110        120 
ESDINFIGME KEETVVGVAL KKTLAEFEQR NKEVTNLKYF NDFAEDLSDI FAPSSIDKIY 

       130        140        150        160        170        180 
LNFSDPWPKA RHSKKRLTYR TFLDIYANII KSHGILEFKT DNDGLFAFSL EEIAENKNWE 

       190        200        210        220 
LIYQTTDLYS DIEALKNNIP TEYETKFHTA GKNINKLIIK KTF 

« Hide

References

[1]Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017263 Genomic DNA. Translation: AAT75575.1.
RefSeqYP_053459.1. NC_006055.1.

3D structure databases

ProteinModelPortalQ6F1P8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265311.Mfl218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT75575; AAT75575; Mfl218.
GeneID2898269.
KEGGmfl:Mfl218.
PATRIC22473161. VBIMesFlo3168_0218.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAFPDPQIK.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK745688.

Enzyme and pathway databases

BioCycMFLO265311:GHIB-239-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MESFL
AccessionPrimary (citable) accession number: Q6F1P8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways