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Reviewed, UniProtKB/Swiss-Prot Q6F1M4 (TMFO1_MESFL)

Last modified June 16, 2009. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO 1
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase 1
    Folate-dependent tRNA(M-5-U54)-methyltransferase 1
Gene names
Name: trmFO1
Ordered Locus Names: Mfl242
OrganismMesoplasma florum (Acholeplasma florum) [Complete proteome] [HAMAP]
Taxonomic identifier2151 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037

Cofactor

FAD By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the mnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO 1 HAMAP MF_01037
PRO_0000346354

Regions

Nucleotide binding9 – 146FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6F1M4-1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: C253B7257CAB22CB

FASTA44249,761
        10         20         30         40         50         60 
MQKEVNIIGA GLAGCEAAYL LANNGVKVNL FEVKSLMKND IQKTNDLGEL VCSNTLRSKS 

        70         80         90        100        110        120 
KKNAAGILKN EMKLLNSLVI KAALENEIPG DDALSVDRFG FSKYITDKIK NHKNINLIEQ 

       130        140        150        160        170        180 
EVSEVDYTKV TIIASGPLTT DKLGKNIELM TGNEKLFFLD ASAPIITKDS IDFNKVYWAS 

       190        200        210        220        230        240 
RHNDGKDGQY ICIPLNEEQF NAFVEELKNA ETIKLKSFEK EIYFKGCQPI EQIAKTSKKV 

       250        260        270        280        290        300 
LLNGPLSPNN LIDENGNTPF AVVQLRQDDA IDSLYNFVGF QTNIKWPEQK RILQTLPGLE 

       310        320        330        340        350        360 
NLNIVRFGVM HKNYYINSPK LLNRSLQVKR NKNIFFAGQI TGVEGYIESA SSGILTAINV 

       370        380        390        400        410        420 
LAYLNNIKIE QPSRKSMLGA LNFYITNPKH DKLKPMKCNL GILDQQNKNA KSEFYSFDES 

       430        440 
EREIRRFIKG INNFAKIGEN NE 

« Hide

References

[1]Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33453 / L1 / NBRC 100688 / NCTC 11704.

Cross-references

Sequence databases

AE017263 Genomic DNA. Translation: AAT75599.1.
RefSeqYP_053483.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID2897836.
GenomeReviewsGene locus Mfl242 in contig AE017263_GR.
KEGGmfl:Mfl242.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ6F1M4.
OMAQ6F1M4. HKNYYIN.

Enzyme and pathway databases

BioCycMFLO265311:MFL242-MON.

Family and domain databases

HAMAPMF_01037.
[Tree]
InterProIPR004417. Gid.
IPR002218. GIDA-rel.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11806. GIDA. 1 hit.
PfamPF01134. GIDA. 1 hit.
[Graphical view]
ProDomPD003738. GIDA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTMFO1_MESFL
AccessionPrimary (citable) accession number: Q6F1M4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 16, 2004
Last modified: June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents