Reviewed, UniProtKB/Swiss-Prot Q6F1M4 (TMFO1_MESFL)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO 1
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase 1
    Folate-dependent tRNA(M-5-U54)-methyltransferase 1

Gene names

Name:	trmFO1
Ordered Locus Names:	Mfl242

Organism

Mesoplasma florum (Acholeplasma florum) [Complete proteome] [HAMAP]

Taxonomic identifier

2151 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Entomoplasmataceae › Mesoplasma

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO 1 HAMAP MF_01037		PRO_0000346354
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F1M4-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: C253B7257CAB22CB	FASTA	442	49,761
	10 20 30 40 50 60 MQKEVNIIGA GLAGCEAAYL LANNGVKVNL FEVKSLMKND IQKTNDLGEL VCSNTLRSKS 70 80 90 100 110 120 KKNAAGILKN EMKLLNSLVI KAALENEIPG DDALSVDRFG FSKYITDKIK NHKNINLIEQ 130 140 150 160 170 180 EVSEVDYTKV TIIASGPLTT DKLGKNIELM TGNEKLFFLD ASAPIITKDS IDFNKVYWAS 190 200 210 220 230 240 RHNDGKDGQY ICIPLNEEQF NAFVEELKNA ETIKLKSFEK EIYFKGCQPI EQIAKTSKKV 250 260 270 280 290 300 LLNGPLSPNN LIDENGNTPF AVVQLRQDDA IDSLYNFVGF QTNIKWPEQK RILQTLPGLE 310 320 330 340 350 360 NLNIVRFGVM HKNYYINSPK LLNRSLQVKR NKNIFFAGQI TGVEGYIESA SSGILTAINV 370 380 390 400 410 420 LAYLNNIKIE QPSRKSMLGA LNFYITNPKH DKLKPMKCNL GILDQQNKNA KSEFYSFDES 430 440 EREIRRFIKG INNFAKIGEN NE

References

[1]

Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33453 / L1 / NBRC 100688 / NCTC 11704.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017263 Genomic DNA. Translation: AAT75599.1.
RefSeq	YP_053483.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2897836.
GenomeReviews	Gene locus Mfl242 in contig AE017263_GR.
KEGG	mfl:Mfl242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6F1M4.
OMA	Q6F1M4. HKNYYIN.
Enzyme and pathway databases
BioCyc	MFLO265311:MFL242-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TMFO1_MESFL

Accession

Primary (citable) accession number: Q6F1M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)