Q6F1M4 (TMFO1_MESFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO 1
EC=2.1.1.74

Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase 1
Folate-dependent tRNA(M-5-U54)-methyltransferase 1

Gene names

Name:	trmFO1
Ordered Locus Names:	Mfl242

Organism

Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)

Taxonomic identifier

265311 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Entomoplasmataceae › Mesoplasma

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity. HAMAP MF_01037
Subcellular location	Cytoplasm By similarity HAMAP MF_01037.
Sequence similarities	Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO 1 HAMAP MF_01037		PRO_0000346354
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F1M4 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: C253B7257CAB22CB	FASTA	442	49,761
	10 20 30 40 50 60 MQKEVNIIGA GLAGCEAAYL LANNGVKVNL FEVKSLMKND IQKTNDLGEL VCSNTLRSKS 70 80 90 100 110 120 KKNAAGILKN EMKLLNSLVI KAALENEIPG DDALSVDRFG FSKYITDKIK NHKNINLIEQ 130 140 150 160 170 180 EVSEVDYTKV TIIASGPLTT DKLGKNIELM TGNEKLFFLD ASAPIITKDS IDFNKVYWAS 190 200 210 220 230 240 RHNDGKDGQY ICIPLNEEQF NAFVEELKNA ETIKLKSFEK EIYFKGCQPI EQIAKTSKKV 250 260 270 280 290 300 LLNGPLSPNN LIDENGNTPF AVVQLRQDDA IDSLYNFVGF QTNIKWPEQK RILQTLPGLE 310 320 330 340 350 360 NLNIVRFGVM HKNYYINSPK LLNRSLQVKR NKNIFFAGQI TGVEGYIESA SSGILTAINV 370 380 390 400 410 420 LAYLNNIKIE QPSRKSMLGA LNFYITNPKH DKLKPMKCNL GILDQQNKNA KSEFYSFDES 430 440 EREIRRFIKG INNFAKIGEN NE

References

[1]

Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017263 Genomic DNA. Translation: AAT75599.1.
RefSeq	YP_053483.1. NC_006055.1.
3D structure databases
ProteinModelPortal	Q6F1M4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2897836.
GenomeReviews	Gene locus Mfl242 in contig AE017263_GR.
KEGG	mfl:Mfl242.
PATRIC	22473211. VBIMesFlo3168_0242.
Phylogenomic databases
HOGENOM	HBG285041.
ProtClustDB	PRK05335.
Enzyme and pathway databases
BioCyc	MFLO265311:MFL242-MONOMER.
Family and domain databases
HAMAP	MF_01037. TrmFO. [Tree]
InterPro	IPR004417. Folate-dep_Ribothymidyl_synth. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
KO	K04094.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00137. Gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TMFO1_MESFL

Accession

Primary (citable) accession number: Q6F1M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program