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Q6F175

- SYI_MESFL

UniProt

Q6F175 - SYI_MESFL

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi882 – 8821ZincUniRule annotation
Metal bindingi885 – 8851ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMFLO265311:GHIB-410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mfl389
OrganismiMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Taxonomic identifieri265311 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma
ProteomesiUP000006647: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908Isoleucine--tRNA ligasePRO_0000098414Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi265311.Mfl389.

Structurei

3D structure databases

ProteinModelPortaliQ6F175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6F175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNIYKDTLL IGQTDFDMRA GLKDKEPVIQ EMWDAKKIYD QKQKLNEGKP
60 70 80 90 100
LFMLHDGPPY ANGDLHIGHA LNKTLKDMII RWKNANGYLA PFIMGWDTHG
110 120 130 140 150
LPIETAVTKM GIDRKQTPAV EFRDMCKDYA LKQVANQANQ FKRLGIFSNS
160 170 180 190 200
DVKYVTLTHD FEVSELRLFQ KMYEKEMVYK ALKPIYWSPS SESALAESEI
210 220 230 240 250
EYKDVKSPTI FVAMKVVEGN AKIDTDTEIV IWTTTPWTIP SNQMAAVGEN
260 270 280 290 300
IEYNIVKAND RKFILASSLV NKVAEQIGWE TFEILDTLKG PEIVGVKYAH
310 320 330 340 350
PLYEQKINPV VIGHHVTDEA GTGIVHTAGG FGEDDYIIVK QHGIEPFAPI
360 370 380 390 400
DDQGKFTNEI AEFDEKLVGV FYEDANKIVG MDLEAKQRLL KLKFVSHSYP
410 420 430 440 450
HDWRTKKPVI YRCTSQWFIG LDKAKNQILA NVDQITTKPE WAKKRLYQVL
460 470 480 490 500
EDRTDWTISR QRLWGVPIVA FYDQNDKLVL NNEILAFAID KIAELGTNAW
510 520 530 540 550
FDKPADTFLP EAYRNKNLKK EKDILDVWFD SGSSAIALSE RFKNLPLPYD
560 570 580 590 600
LYLEGNDQYR GWFNASMINS TIYSGKSPYK KLISHGMTVD EKGNKMSKSL
610 620 630 640 650
GNGIDPIEFA NTQGADILRL WVASTDYTDD QKIGPEIIKQ IGESYRKIRN
660 670 680 690 700
TMRFILANLF DFDPSKDYQT NLTEVDRYAL NNLSVVKNKA SEAYDNLSYN
710 720 730 740 750
QVYNLVVNYV TKDLSSFYLD FIKDILYIEK NDSIRRRQVQ TVLYEQLWML
760 770 780 790 800
IDLLRPILIH TIEEVYQAMV NLNKTDSVHL LDNKKQDFIE SNEFVTKWNN
810 820 830 840 850
IMVLRDDVNK ALEIAREQKI INKGFEATVK VCLKDEFKNI ESTTELEKIF
860 870 880 890 900
IVNSLSFTND CSGLSEQKIA FVGVELKNGT KCERCWGIFD TLINNEICER

CNSVVESL
Length:908
Mass (Da):104,380
Last modified:August 16, 2004 - v1
Checksum:i8DC53125D359C17A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75748.1.
RefSeqiYP_053632.1. NC_006055.1.

Genome annotation databases

EnsemblBacteriaiAAT75748; AAT75748; Mfl389.
GeneIDi2898000.
KEGGimfl:Mfl389.
PATRICi22473517. VBIMesFlo3168_0389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75748.1 .
RefSeqi YP_053632.1. NC_006055.1.

3D structure databases

ProteinModelPortali Q6F175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 265311.Mfl389.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT75748 ; AAT75748 ; Mfl389 .
GeneIDi 2898000.
KEGGi mfl:Mfl389.
PATRICi 22473517. VBIMesFlo3168_0389.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MFLO265311:GHIB-410-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Entry informationi

Entry nameiSYI_MESFL
AccessioniPrimary (citable) accession number: Q6F175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 16, 2004
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3