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Q6F175 (SYI_MESFL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:Mfl389
OrganismMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum) [Reference proteome] [HAMAP]
Taxonomic identifier265311 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma

Protein attributes

Sequence length908 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 908908Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098414

Regions

Motif59 – 6911"HIGH" region HAMAP-Rule MF_02002
Motif595 – 5995"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8821Zinc By similarity
Metal binding8851Zinc By similarity
Metal binding8981Zinc By similarity
Metal binding9011Zinc By similarity
Binding site5541Aminoacyl-adenylate By similarity
Binding site5981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6F175 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 8DC53125D359C17A

FASTA908104,380
        10         20         30         40         50         60 
MKNIYKDTLL IGQTDFDMRA GLKDKEPVIQ EMWDAKKIYD QKQKLNEGKP LFMLHDGPPY 

        70         80         90        100        110        120 
ANGDLHIGHA LNKTLKDMII RWKNANGYLA PFIMGWDTHG LPIETAVTKM GIDRKQTPAV 

       130        140        150        160        170        180 
EFRDMCKDYA LKQVANQANQ FKRLGIFSNS DVKYVTLTHD FEVSELRLFQ KMYEKEMVYK 

       190        200        210        220        230        240 
ALKPIYWSPS SESALAESEI EYKDVKSPTI FVAMKVVEGN AKIDTDTEIV IWTTTPWTIP 

       250        260        270        280        290        300 
SNQMAAVGEN IEYNIVKAND RKFILASSLV NKVAEQIGWE TFEILDTLKG PEIVGVKYAH 

       310        320        330        340        350        360 
PLYEQKINPV VIGHHVTDEA GTGIVHTAGG FGEDDYIIVK QHGIEPFAPI DDQGKFTNEI 

       370        380        390        400        410        420 
AEFDEKLVGV FYEDANKIVG MDLEAKQRLL KLKFVSHSYP HDWRTKKPVI YRCTSQWFIG 

       430        440        450        460        470        480 
LDKAKNQILA NVDQITTKPE WAKKRLYQVL EDRTDWTISR QRLWGVPIVA FYDQNDKLVL 

       490        500        510        520        530        540 
NNEILAFAID KIAELGTNAW FDKPADTFLP EAYRNKNLKK EKDILDVWFD SGSSAIALSE 

       550        560        570        580        590        600 
RFKNLPLPYD LYLEGNDQYR GWFNASMINS TIYSGKSPYK KLISHGMTVD EKGNKMSKSL 

       610        620        630        640        650        660 
GNGIDPIEFA NTQGADILRL WVASTDYTDD QKIGPEIIKQ IGESYRKIRN TMRFILANLF 

       670        680        690        700        710        720 
DFDPSKDYQT NLTEVDRYAL NNLSVVKNKA SEAYDNLSYN QVYNLVVNYV TKDLSSFYLD 

       730        740        750        760        770        780 
FIKDILYIEK NDSIRRRQVQ TVLYEQLWML IDLLRPILIH TIEEVYQAMV NLNKTDSVHL 

       790        800        810        820        830        840 
LDNKKQDFIE SNEFVTKWNN IMVLRDDVNK ALEIAREQKI INKGFEATVK VCLKDEFKNI 

       850        860        870        880        890        900 
ESTTELEKIF IVNSLSFTND CSGLSEQKIA FVGVELKNGT KCERCWGIFD TLINNEICER 


CNSVVESL 

« Hide

References

[1]Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017263 Genomic DNA. Translation: AAT75748.1.
RefSeqYP_053632.1. NC_006055.1.

3D structure databases

ProteinModelPortalQ6F175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265311.Mfl389.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT75748; AAT75748; Mfl389.
GeneID2898000.
KEGGmfl:Mfl389.
PATRIC22473517. VBIMesFlo3168_0389.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycMFLO265311:GHIB-410-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_MESFL
AccessionPrimary (citable) accession number: Q6F175
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries