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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei554Aminoacyl-adenylateUniRule annotation1
Binding sitei598ATPUniRule annotation1
Metal bindingi882ZincUniRule annotation1
Metal bindingi885ZincUniRule annotation1
Metal bindingi898ZincUniRule annotation1
Metal bindingi901ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mfl389
OrganismiMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Taxonomic identifieri265311 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma
Proteomesi
  • UP000006647 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984141 – 908Isoleucine--tRNA ligaseAdd BLAST908

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi265311.Mfl389.

Structurei

3D structure databases

ProteinModelPortaliQ6F175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi595 – 599"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6F175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIYKDTLL IGQTDFDMRA GLKDKEPVIQ EMWDAKKIYD QKQKLNEGKP
60 70 80 90 100
LFMLHDGPPY ANGDLHIGHA LNKTLKDMII RWKNANGYLA PFIMGWDTHG
110 120 130 140 150
LPIETAVTKM GIDRKQTPAV EFRDMCKDYA LKQVANQANQ FKRLGIFSNS
160 170 180 190 200
DVKYVTLTHD FEVSELRLFQ KMYEKEMVYK ALKPIYWSPS SESALAESEI
210 220 230 240 250
EYKDVKSPTI FVAMKVVEGN AKIDTDTEIV IWTTTPWTIP SNQMAAVGEN
260 270 280 290 300
IEYNIVKAND RKFILASSLV NKVAEQIGWE TFEILDTLKG PEIVGVKYAH
310 320 330 340 350
PLYEQKINPV VIGHHVTDEA GTGIVHTAGG FGEDDYIIVK QHGIEPFAPI
360 370 380 390 400
DDQGKFTNEI AEFDEKLVGV FYEDANKIVG MDLEAKQRLL KLKFVSHSYP
410 420 430 440 450
HDWRTKKPVI YRCTSQWFIG LDKAKNQILA NVDQITTKPE WAKKRLYQVL
460 470 480 490 500
EDRTDWTISR QRLWGVPIVA FYDQNDKLVL NNEILAFAID KIAELGTNAW
510 520 530 540 550
FDKPADTFLP EAYRNKNLKK EKDILDVWFD SGSSAIALSE RFKNLPLPYD
560 570 580 590 600
LYLEGNDQYR GWFNASMINS TIYSGKSPYK KLISHGMTVD EKGNKMSKSL
610 620 630 640 650
GNGIDPIEFA NTQGADILRL WVASTDYTDD QKIGPEIIKQ IGESYRKIRN
660 670 680 690 700
TMRFILANLF DFDPSKDYQT NLTEVDRYAL NNLSVVKNKA SEAYDNLSYN
710 720 730 740 750
QVYNLVVNYV TKDLSSFYLD FIKDILYIEK NDSIRRRQVQ TVLYEQLWML
760 770 780 790 800
IDLLRPILIH TIEEVYQAMV NLNKTDSVHL LDNKKQDFIE SNEFVTKWNN
810 820 830 840 850
IMVLRDDVNK ALEIAREQKI INKGFEATVK VCLKDEFKNI ESTTELEKIF
860 870 880 890 900
IVNSLSFTND CSGLSEQKIA FVGVELKNGT KCERCWGIFD TLINNEICER

CNSVVESL
Length:908
Mass (Da):104,380
Last modified:August 16, 2004 - v1
Checksum:i8DC53125D359C17A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75748.1.
RefSeqiWP_011183288.1. NC_006055.1.
YP_053632.1. NC_006055.1.

Genome annotation databases

EnsemblBacteriaiAAT75748; AAT75748; Mfl389.
GeneIDi2898000.
KEGGimfl:Mfl389.
PATRICi22473517. VBIMesFlo3168_0389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75748.1.
RefSeqiWP_011183288.1. NC_006055.1.
YP_053632.1. NC_006055.1.

3D structure databases

ProteinModelPortaliQ6F175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi265311.Mfl389.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT75748; AAT75748; Mfl389.
GeneIDi2898000.
KEGGimfl:Mfl389.
PATRICi22473517. VBIMesFlo3168_0389.

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_MESFL
AccessioniPrimary (citable) accession number: Q6F175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.