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Q6F145 (TYSY_MESFL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:Mfl419
OrganismMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum) [Reference proteome] [HAMAP]
Taxonomic identifier265311 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Entomoplasmatales › Entomoplasmataceae › Mesoplasma ›

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140979

Sites

Active site1701 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6F145 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 39CC3DBB87E6B46D

FASTA28833,889
        10         20         30         40         50         60 
MKQYLELVNE ILKDGEKRED RTNTGTISKF GVQKRYDLRE GFPLLTTKKV FYKAIFHEML 

        70         80         90        100        110        120 
WFIKGDTNIK YLVENNVKIW NEWPYENFKK SSEFNNETLQ EFVERLKNDN EFCNKWGDLG 

       130        140        150        160        170        180 
PVYGKQWRDF GGIDQFAKLI NDIKKNPFSR RHIVSAWNPA EVDNMLLPPC HSFWQVYVSK 

       190        200        210        220        230        240 
DGWLDLQLYQ RSGDVFLGVP FNIASYALLM ELIAKECNLK ARYFVHTIGD AHIYLNHLEQ 

       250        260        270        280 
INEQLKRKPL PLCKIKINSE KSIFDIAFED IEIEGYESHA KITGEVAV

« Hide

References

[1]Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017263 Genomic DNA. Translation: AAT75778.1.
RefSeqYP_053662.1. NC_006055.1.

3D structure databases

ProteinModelPortalQ6F145.
SMRQ6F145. Positions 2-288.
ModBaseSearch...

Protein-protein interaction databases

STRING265311.Mfl419.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT75778; AAT75778; Mfl419.
GeneID2897608.
KEGGmfl:Mfl419.
PATRIC22473585. VBIMesFlo3168_0420.

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMADTNVAYL.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMFLO265311:GHIB-440-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_MESFL
AccessionPrimary (citable) accession number: Q6F145
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: August 16, 2004
Last modified: May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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