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Q6F0X5 (SYL_MESFL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mfl490
OrganismMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum) [Reference proteome] [HAMAP]
Taxonomic identifier265311 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma

Protein attributes

Sequence length801 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 801801Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152041

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif578 – 5825"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5811ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6F0X5 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 7B12CCD8B1BCFFD5

FASTA80192,070
        10         20         30         40         50         60 
MEFSHKAIEK KWKKYWEENN TNKTTNTSDK KSYVLDMFPY PSGAGIHVGH VKGYTATDVF 

        70         80         90        100        110        120 
SRYKRMNGYD VLHPMGWDAF GLPAEQYALK TGNDPIDFTL ENIKTFKRQL KMMGFSYDFD 

       130        140        150        160        170        180 
KEISTANPNY YKITQWIFNQ LYKKGLAENR DVEVNWCQEL GTVLANDEII EKDGLMVSER 

       190        200        210        220        230        240 
GEHPVTKRKM RQWVLKITEY ADRLLEGLDE LEWNSSIKDL QRNWIGKSTG VELDFLVNNI 

       250        260        270        280        290        300 
KVPVFTTRID TIYGVSYIVL APEHEQVLNI TTPEQLKEVQ TYIELAKNKS EIDRKDESKP 

       310        320        330        340        350        360 
KTGVFTGSYA TNPHTNELVQ VWVSDYVLAN YGTGAVMAVP AHDKRDWEFA TKFNLEKKFV 

       370        380        390        400        410        420 
IENKTDEKAF VGEGKIINSD ILNGMDKKQA IQTMTKIAIE QGWGREQTNY KLRDWLFSRQ 

       430        440        450        460        470        480 
RFYGEPFPVL YGPNQEITLI EDLPVELPRI KNIKPSGTGE SPLANVEEWV NVEIDGVKYR 

       490        500        510        520        530        540 
RETNTMPQSA GSSWYYLAYI LADGENEFID IDSAEAKKRF EKWMPVDLYV GGQEHAVGHL 

       550        560        570        580        590        600 
LYARFWNYVL YDLGITSVKE PFKQLFNQGM ILGPDGRKMS KSWGNVINPD DIVSTHGADS 

       610        620        630        640        650        660 
LRLYEMFMGP LDASLPWSED GLDSALKWIH RAYRMVMTTE LTDVNDTKLD FVYNDVVKNV 

       670        680        690        700        710        720 
SEMIESLKFN TAISQLMIFV NAVYKHEGPV YRPYIEGFVK MLSIYAPFIG EELWEKLGHA 

       730        740        750        760        770        780 
PSITKQAWPV FDPSKLVSNT VVIALQINGK LRATIEVEKG TIKDKLLELA KKQESIISYI 

       790        800 
KDKEIIKEIA VVDRIVNIVI K 

« Hide

References

[1]Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017263 Genomic DNA. Translation: AAT75848.1.
RefSeqYP_053732.1. NC_006055.1.

3D structure databases

ProteinModelPortalQ6F0X5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265311.Mfl490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT75848; AAT75848; Mfl490.
GeneID2898120.
KEGGmfl:Mfl490.
PATRIC22473739. VBIMesFlo3168_0496.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMFLO265311:GHIB-510-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 2 hits.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MESFL
AccessionPrimary (citable) accession number: Q6F0X5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: August 16, 2004
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries