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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMFLO265311:GHIB-510-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mfl490
OrganismiMesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)
Taxonomic identifieri265311 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesEntomoplasmataceaeMesoplasma
ProteomesiUP000006647 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 801801Leucine--tRNA ligasePRO_0000152041Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi265311.Mfl490.

Structurei

3D structure databases

ProteinModelPortaliQ6F0X5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi578 – 5825"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 2 hits.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6F0X5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSHKAIEK KWKKYWEENN TNKTTNTSDK KSYVLDMFPY PSGAGIHVGH
60 70 80 90 100
VKGYTATDVF SRYKRMNGYD VLHPMGWDAF GLPAEQYALK TGNDPIDFTL
110 120 130 140 150
ENIKTFKRQL KMMGFSYDFD KEISTANPNY YKITQWIFNQ LYKKGLAENR
160 170 180 190 200
DVEVNWCQEL GTVLANDEII EKDGLMVSER GEHPVTKRKM RQWVLKITEY
210 220 230 240 250
ADRLLEGLDE LEWNSSIKDL QRNWIGKSTG VELDFLVNNI KVPVFTTRID
260 270 280 290 300
TIYGVSYIVL APEHEQVLNI TTPEQLKEVQ TYIELAKNKS EIDRKDESKP
310 320 330 340 350
KTGVFTGSYA TNPHTNELVQ VWVSDYVLAN YGTGAVMAVP AHDKRDWEFA
360 370 380 390 400
TKFNLEKKFV IENKTDEKAF VGEGKIINSD ILNGMDKKQA IQTMTKIAIE
410 420 430 440 450
QGWGREQTNY KLRDWLFSRQ RFYGEPFPVL YGPNQEITLI EDLPVELPRI
460 470 480 490 500
KNIKPSGTGE SPLANVEEWV NVEIDGVKYR RETNTMPQSA GSSWYYLAYI
510 520 530 540 550
LADGENEFID IDSAEAKKRF EKWMPVDLYV GGQEHAVGHL LYARFWNYVL
560 570 580 590 600
YDLGITSVKE PFKQLFNQGM ILGPDGRKMS KSWGNVINPD DIVSTHGADS
610 620 630 640 650
LRLYEMFMGP LDASLPWSED GLDSALKWIH RAYRMVMTTE LTDVNDTKLD
660 670 680 690 700
FVYNDVVKNV SEMIESLKFN TAISQLMIFV NAVYKHEGPV YRPYIEGFVK
710 720 730 740 750
MLSIYAPFIG EELWEKLGHA PSITKQAWPV FDPSKLVSNT VVIALQINGK
760 770 780 790 800
LRATIEVEKG TIKDKLLELA KKQESIISYI KDKEIIKEIA VVDRIVNIVI

K
Length:801
Mass (Da):92,070
Last modified:August 16, 2004 - v1
Checksum:i7B12CCD8B1BCFFD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75848.1.
RefSeqiWP_011183388.1. NC_006055.1.
YP_053732.1. NC_006055.1.

Genome annotation databases

EnsemblBacteriaiAAT75848; AAT75848; Mfl490.
GeneIDi2898120.
KEGGimfl:Mfl490.
PATRICi22473739. VBIMesFlo3168_0496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017263 Genomic DNA. Translation: AAT75848.1.
RefSeqiWP_011183388.1. NC_006055.1.
YP_053732.1. NC_006055.1.

3D structure databases

ProteinModelPortaliQ6F0X5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi265311.Mfl490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT75848; AAT75848; Mfl490.
GeneIDi2898120.
KEGGimfl:Mfl490.
PATRICi22473739. VBIMesFlo3168_0496.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMFLO265311:GHIB-510-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 2 hits.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Entry informationi

Entry nameiSYL_MESFL
AccessioniPrimary (citable) accession number: Q6F0X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: August 16, 2004
Last modified: May 27, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.