Q6F0I2 (KITH_MESFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	Mfl635

Organism

Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum) [Reference proteome] [HAMAP]

Taxonomic identifier

265311 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Entomoplasmataceae › Mesoplasma › Mesoplasma florum

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000174992
Regions
	Nucleotide binding	21 – 28	8	ATP By similarity
	Nucleotide binding	99 – 102	4	ATP By similarity
Sites
	Active site	100	1	Proton acceptor Potential
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	194	1	Zinc By similarity
	Metal binding	197	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F0I2 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: B4871C2CC2B2D487	FASTA	203	22,998
	10 20 30 40 50 60 MIPNRDTIEQ KQLGWVELIT GCMFAGKTEE FIKRLRRHAF AKRNVIAFKP VIDTRYAVNE 70 80 90 100 110 120 VASHAGTLLP SIPVNSTAEL KEKLEAKILE KKVDVVGIDE IQFFDEAIVD YIEELADRGI 130 140 150 160 170 180 IVIVTGLDKD FRSQPFKNVD RILPLAEMVD KLTAICQKCG NFANRTQRII DGKPADWNSP 190 200 LILVDGNDSY EARCRNCYQI EKG

References

[1]

Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017263 Genomic DNA. Translation: AAT75991.1.
RefSeq	YP_053875.1. NC_006055.1.
3D structure databases
ProteinModelPortal	Q6F0I2.
ModBase	Search...
Protein-protein interaction databases
STRING	265311.Mfl635.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT75991; AAT75991; Mfl635.
GeneID	2897887.
KEGG	mfl:Mfl635.
PATRIC	22474049. VBIMesFlo3168_0637.
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076390.
KO	K00857.
OMA	KVHAICV.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	MFLO265311:GHIB-653-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_MESFL

Accession

Primary (citable) accession number: Q6F0I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program