Q6F0G6 (SYE_MESFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Mfl651

Organism

Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)

Taxonomic identifier

265311 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Entomoplasmataceae › Mesoplasma

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119596
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	253 – 257	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	256	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6F0G6 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: E70EC7D196D1EF46	FASTA	482	55,805
	10 20 30 40 50 60 MTKFRLRYAP SPTGFLHIGN TRTALMNYLF AKHYNGDFIV RIEDTDLERN VEGAIESQFE 70 80 90 100 110 120 NLNWLGINAD ESFLKPGEEK YGKYMQSQKF GRYQELAEKL ISIKKAYRCF CTTEELEKDY 130 140 150 160 170 180 EDQVAKGIVA TKYSGKCSRL SESEIESNLK SNKDFSIRFL VPETTLNIKD FIKGEITFDS 190 200 210 220 230 240 KELGDFVILK TNKIATYNFA VVVDDFDMEI SHVLRGEEHI SNTPRQILIY QAFGWETPQF 250 260 270 280 290 300 GHMSLIVDST GKKLSKRSGN ALFFIEQYKN QGYLPEAMFN YISLLGWSPI GEKELLTKQE 310 320 330 340 350 360 LISMFDDKRF SKSPSTFDMT KMKWINSQYM KALSEEEYLE FTKKYINTEM FNTKEKSEDW 370 380 390 400 410 420 LNQVLLLFKK ELEFADQINN HLNIFFNEVD VTSETIEILK TIEEHESVIK EFETQISTLN 430 440 450 460 470 480 EWEIENIKSL IKLVSENTGK KGKDLFMPIR IASSSSEHGP SLADVIYLLG KDKVLANIAK VK

References

[1]

Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S., Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33453 / NBRC 100688 / NCTC 11704 / L1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017263 Genomic DNA. Translation: AAT76007.1.
RefSeq	YP_053891.1. NC_006055.1.
3D structure databases
ProteinModelPortal	Q6F0G6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2898181.
GenomeReviews	Gene locus Mfl651 in contig AE017263_GR.
KEGG	mfl:Mfl651.
PATRIC	22474081. VBIMesFlo3168_0653.
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DDFDMEI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	MFLO265311:MFL651-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MESFL

Accession

Primary (citable) accession number: Q6F0G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program