Reviewed, UniProtKB/Swiss-Prot Q6EX42 (PI5K1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-4-phosphate 5-kinase 1
    EC=2.7.1.68
Alternative name(s):
    1-phosphatidylinositol-4-phosphate kinase
    PIP5K
    PtdIns(4)P-5-kinase
    Diphosphoinositide kinase

Gene names

Name:	PIPK1
Ordered Locus Names:	Os03g0701800, LOC_Os03g49510

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in flowering. May suppress floral initiation by modifying the expression of genes related to floral induction. Ref.1
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Tissue specificity	Expressed in young seedlings, shoot and seeds, and at lower level in roots, stem and leaf. Ref.1
Induction	By auxin, abscisic acid, cytokinins, gibberellic acid, Ca2+, methyl jasmonate and salicylic acid. Ref.1
Miscellaneous	Transgenic plants suppressing PIPK1 show earlier heading (7 to 14 days earlier), delayed leaf development (a common phenotype observed with earlier flowering), and greater expression of floral induction genes.
Sequence similarities	Contains 8 MORN repeats. Contains 1 PIPK domain.

Ontologies

Keywords
Biological process	Differentiation Flowering
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Transferase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol-4-phosphate 5-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6EX42-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6EX42-2)

The sequence of this isoform differs from the canonical sequence as follows:
     494-502: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 801	780	Phosphatidylinositol-4-phosphate 5-kinase 1		PRO_0000024105
Regions
	Repeat	41 – 63	23	MORN 1
	Repeat	64 – 86	23	MORN 2
	Repeat	87 – 109	23	MORN 3
	Repeat	110 – 132	23	MORN 4
	Repeat	133 – 155	23	MORN 5
	Repeat	156 – 178	23	MORN 6
	Repeat	182 – 201	20	MORN 7
	Repeat	202 – 223	22	MORN 8
	Domain	366 – 797	432	PIPK
Natural variations
	Alternative sequence	494 – 502	9	Missing in isoform 2.		VSP_015135

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: A72DED14A54CDCE6	FASTA	801	90,680
	10 20 30 40 50 60 MPGLHVVSFL VVLLLQLRSS GMHLVASELF WGNTLPNGDI YVGSFDGLVP HGPGKYMWTD 70 80 90 100 110 120 GALYDGEWDK SKMTGRGLIQ WPSGASYEGD FRGGFIDGAG TFKGVDGSVY KGSWRMNKKH 130 140 150 160 170 180 GMGTMVYSNS DTYEGFWNEG LPDEFGKYTW ADGNVYIGRW KSGKMNGSGV MQWINGDTLD 190 200 210 220 230 240 CNWLNGLAHG KGYCKYASGA CYIGTWDRGL KDGHGTFYQP GSKIPCNLEV SDCLTSHDGT 250 260 270 280 290 300 SASSSSNEKI TIGLLFLLQK LCKNWRLRRF LHRPRRISNG TTPVFDDNSG SHLCQDVSSK 310 320 330 340 350 360 SFSADDQCLQ DSEVDKDSVY EREYVQGVLI MEQPKNEDSR MSESGIAQEN NWEKQAKGPM 370 380 390 400 410 420 ETIYKGHRSY YLMLNLQLGI RYTVGKITPV PLREVRSNDF GPRARIKMYF PCEGSQYTPP 430 440 450 460 470 480 HYSVDFFWKD YCPMVFRNLR EMFHIDAADY MMSICGGDSL KELSSPGKSG SIFYLSQDER 490 500 510 520 530 540 FVIKTLRKTE LKIGLMKYVL QILLKMLPKY YNHVKAYDNT LITKFFGVHR ITLKPGRKVR 550 560 570 580 590 600 FVVMGNMFCT ELRIHRKYDL KGSTQGRSTK KQNINENTTL KDLDLSYVFH VDKPWREALF 610 620 630 640 650 660 RQIALDCMFL ESQSIIDYSM LLGIHFRAPN HLKRITSCQN ALESTGISAE TECSVALHHE 670 680 690 700 710 720 ETISSKGFLL VAADEPGPAV RGSHIRGSMV RAAEGGYEEV DLVLPGTGRF RVQLGVNMPA 730 740 750 760 770 780 RARKVQEDVN VEVENRDTIE EYDVVLYLGI IDILQEYNVS KRVEHAVKSL KFDPLSISAV 790 800 DPNLYSRRFI SFLEKVFPEQ D
	Isoform 2. Checksum: C63D276B87126611	FASTA	792	89,634
	10 20 30 40 50 60 MPGLHVVSFL VVLLLQLRSS GMHLVASELF WGNTLPNGDI YVGSFDGLVP HGPGKYMWTD 70 80 90 100 110 120 GALYDGEWDK SKMTGRGLIQ WPSGASYEGD FRGGFIDGAG TFKGVDGSVY KGSWRMNKKH 130 140 150 160 170 180 GMGTMVYSNS DTYEGFWNEG LPDEFGKYTW ADGNVYIGRW KSGKMNGSGV MQWINGDTLD 190 200 210 220 230 240 CNWLNGLAHG KGYCKYASGA CYIGTWDRGL KDGHGTFYQP GSKIPCNLEV SDCLTSHDGT 250 260 270 280 290 300 SASSSSNEKI TIGLLFLLQK LCKNWRLRRF LHRPRRISNG TTPVFDDNSG SHLCQDVSSK 310 320 330 340 350 360 SFSADDQCLQ DSEVDKDSVY EREYVQGVLI MEQPKNEDSR MSESGIAQEN NWEKQAKGPM 370 380 390 400 410 420 ETIYKGHRSY YLMLNLQLGI RYTVGKITPV PLREVRSNDF GPRARIKMYF PCEGSQYTPP 430 440 450 460 470 480 HYSVDFFWKD YCPMVFRNLR EMFHIDAADY MMSICGGDSL KELSSPGKSG SIFYLSQDER 490 500 510 520 530 540 FVIKTLRKTE LKILLKMLPK YYNHVKAYDN TLITKFFGVH RITLKPGRKV RFVVMGNMFC 550 560 570 580 590 600 TELRIHRKYD LKGSTQGRST KKQNINENTT LKDLDLSYVF HVDKPWREAL FRQIALDCMF 610 620 630 640 650 660 LESQSIIDYS MLLGIHFRAP NHLKRITSCQ NALESTGISA ETECSVALHH EETISSKGFL 670 680 690 700 710 720 LVAADEPGPA VRGSHIRGSM VRAAEGGYEE VDLVLPGTGR FRVQLGVNMP ARARKVQEDV 730 740 750 760 770 780 NVEVENRDTI EEYDVVLYLG IIDILQEYNV SKRVEHAVKS LKFDPLSISA VDPNLYSRRF 790 ISFLEKVFPE QD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"OsPIPK 1, a rice phosphatidylinositol monophosphate kinase, regulates rice heading by modifying the expression of floral induction genes." Ma H., Xu S.-P., Luo D., Xu Z.-H., Xue H.-W. Plant Mol. Biol. 54:295-310(2004) [PubMed: 15159629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [MRNA] OF 112-801 (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Zhonghua 11.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ439082 mRNA. Translation: CAD27794.1. AJ544688 mRNA. Translation: CAD67588.1. AC091670 Genomic DNA. Translation: AAX95508.1.
RefSeq	NP_001051005.1.
UniGene	Os.38046
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4333833.
KEGG	osa:4333833.
Organism-specific databases
Gramene	Q6EX42.
Family and domain databases
InterPro	IPR003409. MORN. IPR017163. PIno-4-P-5_kinase_pln. IPR002498. PInositol-4-P-5-kinase_core. IPR016034. PInositol-4P-5-kinase_core_sub. [Graphical view]
PANTHER	PTHR23086. PIP5K. 1 hit.
Pfam	PF02493. MORN. 8 hits. PF01504. PIP5K. 1 hit. [Graphical view]
PIRSF	PIRSF037274. PIP5K_plant_prd. 1 hit.
SMART	SM00698. MORN. 8 hits. SM00330. PIPKc. 1 hit. [Graphical view]
PROSITE	PS51455. PIPK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI5K1_ORYSJ

Accession

Primary (citable) accession number: Q6EX42
Secondary accession number(s): Q70Z13

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	October 13, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)