Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein kinase fyna

Gene

fyna

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Role in cntn1-mediated signaling (By similarity). Required for convergent extension cell movements during gastrulation, acting with yes via rhoa. May be required for epiboly to occur, possibly through its effects in calcium signaling.By similarity2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Enzyme regulationi

Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site (By similarity). Relatively inactive in the unfertilized oocyte, undergoes rapid activation immediately following fertilization. Total activity increases progressively during later development and remains elevated during sphere and epiboly stage.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei299ATPPROSITE-ProRule annotation1
Active sitei390Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi277 – 285ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • adherens junction maintenance Source: ZFIN
  • cell differentiation Source: GO_Central
  • cell migration Source: GO_Central
  • central nervous system development Source: GO_Central
  • convergent extension involved in gastrulation Source: ZFIN
  • gastrulation with mouth forming second Source: ZFIN
  • innate immune response Source: GO_Central
  • myelination Source: ZFIN
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • protein autophosphorylation Source: ZFIN
  • regulation of apoptotic process Source: GO_Central
  • regulation of cell proliferation Source: GO_Central
  • regulation of cytosolic calcium ion concentration Source: ZFIN
  • regulation of protein kinase activity Source: ZFIN
  • T cell activation Source: GO_Central
  • T cell receptor signaling pathway Source: GO_Central
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-114604. GPVI-mediated activation cascade.
R-DRE-1433557. Signaling by SCF-KIT.
R-DRE-202733. Cell surface interactions at the vascular wall.
R-DRE-2029481. FCGR activation.
R-DRE-2424491. DAP12 signaling.
R-DRE-373753. Nephrin interactions.
R-DRE-375165. NCAM signaling for neurite out-growth.
R-DRE-3928662. EPHB-mediated forward signaling.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-3928664. Ephrin signaling.
R-DRE-3928665. EPH-ephrin mediated repulsion of cells.
R-DRE-399954. Sema3A PAK dependent Axon repulsion.
R-DRE-399955. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.
R-DRE-399956. CRMPs in Sema3A signaling.
R-DRE-418885. DCC mediated attractive signaling.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5621575. CD209 (DC-SIGN) signaling.
R-DRE-5673001. RAF/MAP kinase cascade.
R-DRE-75892. Platelet Adhesion to exposed collagen.
R-DRE-8866376. Reelin signalling pathway.
R-DRE-912631. Regulation of signaling by CBL.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase fyna (EC:2.7.10.2)
Alternative name(s):
Proto-oncogene c-Fyna
Gene namesi
Name:fyna
Synonyms:fyn
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 17

Organism-specific databases

ZFINiZDB-GENE-030903-5. fyna.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Transiently expressed in the nucleus at mid-blastula stage (3.3 hpf) in a large subset of cells. Nuclear localization is not observed from late blastula stage onward.

GO - Cellular componenti

  • cytosol Source: ZFIN
  • extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
  • nucleus Source: ZFIN
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi299K → M: Loss of activity. 1 Publication1

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004188782 – 537Tyrosine-protein kinase fynaAdd BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi3S-palmitoyl cysteineBy similarity1
Lipidationi6S-palmitoyl cysteineBy similarity1
Modified residuei12Phosphothreonine; by PKCBy similarity1
Modified residuei420Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei531PhosphotyrosineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ6EWH2.
PRIDEiQ6EWH2.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Developmental stagei

Expressed in the pre-gastrula embryo (at protein level).

Gene expression databases

BgeeiENSDARG00000011370.
ExpressionAtlasiQ6EWH2. differential.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000123888.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 143SH3PROSITE-ProRule annotationAdd BLAST62
Domaini149 – 246SH2PROSITE-ProRule annotationAdd BLAST98
Domaini271 – 524Protein kinasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118938.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ6EWH2.
OMAiSHNSGYR.
OrthoDBiEOG091G0D46.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6EWH2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCVQCKDKE ATKLTDERET SVSQHAGYRY GSDPTPQHYP SFGVTAIPNY
60 70 80 90 100
NNFHAPVSQG VTVFGGVNSS SHSGTLRSRG GTGVTLFVAL YDYEARSEDD
110 120 130 140 150
LSFRKGEKFQ ILNSTEGDWW EARSLTTGGT GYIPSNYVAP VDSIQAEDWY
160 170 180 190 200
FGKLGRKDAE RQLLSNGNPR GTFLIRESET TKGAYSLSIQ DWDETKGDHV
210 220 230 240 250
KHYKIRKLDN GGYYITTRAQ FETLQQLVHH YSARAAGLCC RLIVPCHKGM
260 270 280 290 300
PRLADLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAVKT
310 320 330 340 350
LKPGTMSPES FLEEAQIMKK LRHDKLVQLY AVVSEEPIYI VTEYMSKGSL
360 370 380 390 400
LDFLKDGEGR GLKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGD
410 420 430 440 450
SLVCKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW
460 470 480 490 500
SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPSSLHELML
510 520 530
QCWKRDPEER PTFEYLQAFL EDYFTATEPQ YQPGDNL
Length:537
Mass (Da):60,569
Last modified:September 5, 2012 - v2
Checksum:i1C7CB34990049B96
GO
Isoform 2 (identifier: Q6EWH2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     293-293: N → H
     294-537: Missing.

Note: No experimental confirmation available.
Show »
Length:293
Mass (Da):32,799
Checksum:iCC69174A2FA24017
GO

Sequence cautioni

The sequence AAX47959 differs from that shown. Reason: Frameshift at positions 126 and 131.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5Q → R in AAH75763 (Ref. 4) Curated1
Sequence conflicti60 – 62GVT → RVP in CAF06179 (PubMed:15815683).Curated3
Sequence conflicti148D → E in AAX47959 (PubMed:16112104).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044087293N → H in isoform 2. 1 Publication1
Alternative sequenceiVSP_044088294 – 537Missing in isoform 2. 1 PublicationAdd BLAST244

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY948197 mRNA. Translation: AAX47959.1. Frameshift.
AJ620748 mRNA. Translation: CAF06179.1.
CU306817 Genomic DNA. No translation available.
BC075763 mRNA. Translation: AAH75763.1.
AF269145 mRNA. Translation: AAF81748.1.
RefSeqiNP_001315092.1. NM_001328163.1. [Q6EWH2-1]
UniGeneiDr.118811.

Genome annotation databases

EnsembliENSDART00000046414; ENSDARP00000046413; ENSDARG00000011370. [Q6EWH2-2]
ENSDART00000150232; ENSDARP00000123888; ENSDARG00000011370. [Q6EWH2-1]
GeneIDi373872.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY948197 mRNA. Translation: AAX47959.1. Frameshift.
AJ620748 mRNA. Translation: CAF06179.1.
CU306817 Genomic DNA. No translation available.
BC075763 mRNA. Translation: AAH75763.1.
AF269145 mRNA. Translation: AAF81748.1.
RefSeqiNP_001315092.1. NM_001328163.1. [Q6EWH2-1]
UniGeneiDr.118811.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000123888.

Proteomic databases

PaxDbiQ6EWH2.
PRIDEiQ6EWH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000046414; ENSDARP00000046413; ENSDARG00000011370. [Q6EWH2-2]
ENSDART00000150232; ENSDARP00000123888; ENSDARG00000011370. [Q6EWH2-1]
GeneIDi373872.

Organism-specific databases

CTDi373872.
ZFINiZDB-GENE-030903-5. fyna.

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118938.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ6EWH2.
OMAiSHNSGYR.
OrthoDBiEOG091G0D46.
TreeFamiTF351634.

Enzyme and pathway databases

ReactomeiR-DRE-114604. GPVI-mediated activation cascade.
R-DRE-1433557. Signaling by SCF-KIT.
R-DRE-202733. Cell surface interactions at the vascular wall.
R-DRE-2029481. FCGR activation.
R-DRE-2424491. DAP12 signaling.
R-DRE-373753. Nephrin interactions.
R-DRE-375165. NCAM signaling for neurite out-growth.
R-DRE-3928662. EPHB-mediated forward signaling.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-3928664. Ephrin signaling.
R-DRE-3928665. EPH-ephrin mediated repulsion of cells.
R-DRE-399954. Sema3A PAK dependent Axon repulsion.
R-DRE-399955. SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion.
R-DRE-399956. CRMPs in Sema3A signaling.
R-DRE-418885. DCC mediated attractive signaling.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5621575. CD209 (DC-SIGN) signaling.
R-DRE-5673001. RAF/MAP kinase cascade.
R-DRE-75892. Platelet Adhesion to exposed collagen.
R-DRE-8866376. Reelin signalling pathway.
R-DRE-912631. Regulation of signaling by CBL.

Miscellaneous databases

PROiQ6EWH2.

Gene expression databases

BgeeiENSDARG00000011370.
ExpressionAtlasiQ6EWH2. differential.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFYNA_DANRE
AccessioniPrimary (citable) accession number: Q6EWH2
Secondary accession number(s): F8W4M2
, Q58HR4, Q6DI27, Q9I8J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 5, 2012
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.