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Q6EWH2

- FYNA_DANRE

UniProt

Q6EWH2 - FYNA_DANRE

Protein

Tyrosine-protein kinase fyna

Gene

fyna

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (05 Sep 2012)
      Previous versions | rss
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    Functioni

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Role in cntn1-mediated signaling By similarity. Required for convergent extension cell movements during gastrulation, acting with yes via rhoa. May be required for epiboly to occur, possibly through its effects in calcium signaling.By similarity2 Publications

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Cofactori

    Manganese.

    Enzyme regulationi

    Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site By similarity. Relatively inactive in the unfertilized oocyte, undergoes rapid activation immediately following fertilization. Total activity increases progressively during later development and remains elevated during sphere and epiboly stage.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei299 – 2991ATPPROSITE-ProRule annotation
    Active sitei390 – 3901Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi277 – 2859ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein kinase activity Source: ZFIN

    GO - Biological processi

    1. convergent extension involved in gastrulation Source: ZFIN
    2. cytosolic calcium ion homeostasis Source: ZFIN
    3. gastrulation with mouth forming second Source: ZFIN
    4. protein autophosphorylation Source: ZFIN
    5. regulation of protein kinase activity Source: ZFIN

    Keywords - Molecular functioni

    Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_174907. Regulation of KIT signaling.
    REACT_175016. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_181648. Signaling by SCF-KIT.
    REACT_182699. DAP12 signaling.
    REACT_182784. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_210358. Regulation of signaling by CBL.
    REACT_214033. Netrin mediated repulsion signals.
    REACT_221534. FCGR activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase fyna (EC:2.7.10.2)
    Alternative name(s):
    Proto-oncogene c-Fyna
    Gene namesi
    Name:fyna
    Synonyms:fyn
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 17

    Organism-specific databases

    ZFINiZDB-GENE-030903-5. fyna.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Transiently expressed in the nucleus at mid-blastula stage (3.3 hpf) in a large subset of cells. Nuclear localization is not observed from late blastula stage onward.

    GO - Cellular componenti

    1. cytosol Source: ZFIN
    2. nucleus Source: ZFIN

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi299 – 2991K → M: Loss of activity. 1 Publication

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 537536Tyrosine-protein kinase fynaPRO_0000418878Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteineBy similarity
    Lipidationi6 – 61S-palmitoyl cysteineBy similarity
    Modified residuei12 – 121Phosphothreonine; by PKCBy similarity
    Modified residuei420 – 4201Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei531 – 5311PhosphotyrosineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Expressioni

    Tissue specificityi

    Widely expressed.2 Publications

    Developmental stagei

    Expressed in the pre-gastrula embryo (at protein level).

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000003208.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini82 – 14362SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini149 – 24698SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini271 – 524254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    GeneTreeiENSGT00620000087702.
    HOVERGENiHBG008761.
    InParanoidiQ6EWH2.
    KOiK05703.
    OMAiSHNSGYR.
    OrthoDBiEOG7GTT2V.
    TreeFamiTF351634.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6EWH2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGCVQCKDKE ATKLTDERET SVSQHAGYRY GSDPTPQHYP SFGVTAIPNY    50
    NNFHAPVSQG VTVFGGVNSS SHSGTLRSRG GTGVTLFVAL YDYEARSEDD 100
    LSFRKGEKFQ ILNSTEGDWW EARSLTTGGT GYIPSNYVAP VDSIQAEDWY 150
    FGKLGRKDAE RQLLSNGNPR GTFLIRESET TKGAYSLSIQ DWDETKGDHV 200
    KHYKIRKLDN GGYYITTRAQ FETLQQLVHH YSARAAGLCC RLIVPCHKGM 250
    PRLADLSVKT KDVWEIPRES LQLIKRLGNG QFGEVWMGTW NGNTKVAVKT 300
    LKPGTMSPES FLEEAQIMKK LRHDKLVQLY AVVSEEPIYI VTEYMSKGSL 350
    LDFLKDGEGR GLKLPNLVDM AAQVAAGMAY IERMNYIHRD LRSANILVGD 400
    SLVCKIADFG LARLIEDNEY TARQGAKFPI KWTAPEAALY GRFTIKSDVW 450
    SFGILLTELV TKGRVPYPGM NNREVLEQVE RGYRMPCPQD CPSSLHELML 500
    QCWKRDPEER PTFEYLQAFL EDYFTATEPQ YQPGDNL 537
    Length:537
    Mass (Da):60,569
    Last modified:September 5, 2012 - v2
    Checksum:i1C7CB34990049B96
    GO
    Isoform 2 (identifier: Q6EWH2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         293-293: N → H
         294-537: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:293
    Mass (Da):32,799
    Checksum:iCC69174A2FA24017
    GO

    Sequence cautioni

    The sequence AAX47959.1 differs from that shown. Reason: Frameshift at positions 126 and 131.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51Q → R in AAH75763. 1 PublicationCurated
    Sequence conflicti60 – 623GVT → RVP in CAF06179. (PubMed:15815683)Curated
    Sequence conflicti148 – 1481D → E in AAX47959. (PubMed:16112104)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei293 – 2931N → H in isoform 2. 1 PublicationVSP_044087
    Alternative sequencei294 – 537244Missing in isoform 2. 1 PublicationVSP_044088Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY948197 mRNA. Translation: AAX47959.1. Frameshift.
    AJ620748 mRNA. Translation: CAF06179.1.
    CU306817 Genomic DNA. No translation available.
    BC075763 mRNA. Translation: AAH75763.1.
    AF269145 mRNA. Translation: AAF81748.1.
    RefSeqiNP_001007287.1. NM_001007286.1.
    XP_005169873.1. XM_005169816.1. [Q6EWH2-1]
    UniGeneiDr.118811.

    Genome annotation databases

    EnsembliENSDART00000046414; ENSDARP00000046413; ENSDARG00000011370. [Q6EWH2-2]
    ENSDART00000150232; ENSDARP00000123888; ENSDARG00000011370. [Q6EWH2-1]
    GeneIDi373872.
    KEGGidre:373872.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY948197 mRNA. Translation: AAX47959.1 . Frameshift.
    AJ620748 mRNA. Translation: CAF06179.1 .
    CU306817 Genomic DNA. No translation available.
    BC075763 mRNA. Translation: AAH75763.1 .
    AF269145 mRNA. Translation: AAF81748.1 .
    RefSeqi NP_001007287.1. NM_001007286.1.
    XP_005169873.1. XM_005169816.1. [Q6EWH2-1 ]
    UniGenei Dr.118811.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000003208.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000046414 ; ENSDARP00000046413 ; ENSDARG00000011370 . [Q6EWH2-2 ]
    ENSDART00000150232 ; ENSDARP00000123888 ; ENSDARG00000011370 . [Q6EWH2-1 ]
    GeneIDi 373872.
    KEGGi dre:373872.

    Organism-specific databases

    CTDi 373872.
    ZFINi ZDB-GENE-030903-5. fyna.

    Phylogenomic databases

    GeneTreei ENSGT00620000087702.
    HOVERGENi HBG008761.
    InParanoidi Q6EWH2.
    KOi K05703.
    OMAi SHNSGYR.
    OrthoDBi EOG7GTT2V.
    TreeFami TF351634.

    Enzyme and pathway databases

    Reactomei REACT_174907. Regulation of KIT signaling.
    REACT_175016. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_181648. Signaling by SCF-KIT.
    REACT_182699. DAP12 signaling.
    REACT_182784. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_210358. Regulation of signaling by CBL.
    REACT_214033. Netrin mediated repulsion signals.
    REACT_221534. FCGR activation.

    Miscellaneous databases

    NextBioi 20813409.
    PROi Q6EWH2.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Role of Fyn kinase in signaling associated with epiboly during zebrafish development."
      Sharma D., Holets L., Zhang X., Kinsey W.H.
      Dev. Biol. 285:462-476(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, MUTAGENESIS OF LYS-299.
      Tissue: Embryo.
    2. "Fyn/Yes and non-canonical Wnt signalling converge on RhoA in vertebrate gastrulation cell movements."
      Jopling C., den Hertog J.
      EMBO Rep. 6:426-431(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    3. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    4. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Embryo.
    5. "Transient nuclear localization of Fyn kinase during development in zebrafish."
      Rongish B.J., Kinsey W.H.
      Anat. Rec. 260:115-123(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-153, SUBCELLULAR LOCATION, ENZYME REGULATION, TISSUE SPECIFICITY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiFYNA_DANRE
    AccessioniPrimary (citable) accession number: Q6EWH2
    Secondary accession number(s): F8W4M2
    , Q58HR4, Q6DI27, Q9I8J8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: September 5, 2012
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3