Reviewed, UniProtKB/Swiss-Prot Q6EV76 (FUT8_RAT)

Names and origin

Protein names

Recommended name:
    Alpha-(1,6)-fucosyltransferase
    EC=2.4.1.68
Alternative name(s):
    Glycoprotein 6-alpha-L-fucosyltransferase
    GDP-fucose--glycoprotein fucosyltransferase
    GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
      Short name=alpha1-6FucT

Gene names

Name:

Fut8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans By similarity.
Catalytic activity	GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 23 family. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	SH3 domain SH3-binding Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	protein amino acid glycosylation in Golgi Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW glycoprotein 6-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Alpha-(1,6)-fucosyltransferase		PRO_0000357042
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Signal-anchor for type II membrane protein Potential
	Topological domain	31 – 575	545	Lumenal Potential
	Domain	502 – 563	62	SH3
	Region	365 – 366	2	Important for donor substrate binding
	Motif	299 – 305	7	SH3-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6EV76-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 731EFB6D850B76CC	FASTA	575	66,485
	10 20 30 40 50 60 MRAWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHPDHSS RELSKILAKL ERLKQQNEDL 70 80 90 100 110 120 RRMAESLRIP EGPIDQGTAT GRVRVLEEQL VKAKEQIENY KKQARNGLGK DHELLRRRIE 130 140 150 160 170 180 NGAKELWFFL QSELKKLKHL EGNELQRHAD EILLDLGHHE RSIMTDLYYL SQTDGAGDWR 190 200 210 220 230 240 EKEAKDLTEL VQRRITYLQN PKDCSKARKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT 250 260 270 280 290 300 LILESQNWRY ATGGWETVFR PVSETCTDRS GLSTGHWSGE VNDKNIQVVE LPIVDSLHPR 310 320 330 340 350 360 PPYLPLAVPE DLADRLVRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT KKLGFKHPVI 370 380 390 400 410 420 GVHVRRTDKV GTEAAFHPIE EYMVHVEEHF QLLARRMQVD KKRVYLATDD PALLKEAKTK 430 440 450 460 470 480 YSNYEFISDN SISWSAGLHN RYTENSLRGV ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ 490 500 510 520 530 540 TLHPDASANF HSLDDIYYFG GQNAHNQIAV YPHKPRTDEE IPMEPGDIIG VAGNHWDGYS 550 560 570 KGVNRKLGKT GLYPSYKVRE KIETVKYPTY PEAEK

References

[1]	"Phylogeny of fucosyltransferases." Martinez-Duncker I., Oriol R., Mollicone R. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ781406 mRNA. Translation: CAH03674.1.
IPI	IPI00454556.
RefSeq	NP_001002289.1.
UniGene	Rn.1717
3D structure databases
SMR	Q6EV76. Positions 108-572.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6EV76.
Protein family/group databases
CAZy	GT23. Glycosyltransferase Family 23.
Proteomic databases
PRIDE	Q6EV76.
Genome annotation databases
Ensembl	ENSRNOT00000011220; ENSRNOP00000011220; ENSRNOG00000008451; Rattus norvegicus. [Genome view]
GeneID	432392.
KEGG	rno:432392.
UCSC	NM_001002289. rat.
Organism-specific databases
CTD	432392.
RGD	1303096. Fut8.
Phylogenomic databases
HOVERGEN	Q6EV76.
Gene expression databases
Genevestigator	Q6EV76.
Family and domain databases
InterPro	IPR015827. Alpha1_6FUT_euk. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMART	SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50002. SH3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	696858.

Entry information

Entry name

FUT8_RAT

Accession

Primary (citable) accession number: Q6EV76

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	August 16, 2004
Last modified:	October 13, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)