Reviewed, UniProtKB/Swiss-Prot Q6EV69 (OFUT1_PANTR)

Names and origin

Protein names

Recommended name:
    GDP-fucose protein O-fucosyltransferase 1
    EC=2.4.1.221
Alternative name(s):
    Peptide-O-fucosyltransferase 1
      Short name=O-FucT-1

Gene names

Name:	POFUT1
Synonyms:	FUT12

Organism

Pan troglodytes (Chimpanzee)

Taxonomic identifier

9598 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pan

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling By similarity.
Catalytic activity	Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the glycosyltransferase 68 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism Notch signaling pathway
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW fucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide-O-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 388	362	GDP-fucose protein O-fucosyltransferase 1		PRO_0000012150
Regions
	Motif	385 – 388	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	160	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6EV69-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 3FACCCA434D02415	FASTA	388	43,956
	10 20 30 40 50 60 MGAAAWARPL SVSFLLLLLP LPGMPAGSWD PAGYLLYCPC MGRFGNQADH FLGSLAFAKL 70 80 90 100 110 120 LNRTLAVPPW IEYQHHKPPF TNLHVSYQKY FKLEPLQAYH RVISLEDFME KLAPTHWPPE 130 140 150 160 170 180 KRVAYCFEVA AQRSPDKKTC PMKEGNPFGP FWDQFHVSFN KSELFTGISF SASYREQWSQ 190 200 210 220 230 240 RFSPKEHPVL ALPGAPAQFP VLEEHRPLQK YMVWSDEMVK TGEAQIHAHL VRPYVGIHLR 250 260 270 280 290 300 IGSDWKNACA MLKDGTAGSH FMASPQCVGY SRSTAAPLTM TMCLPDLKEI QRAVKLWVRS 310 320 330 340 350 360 LDAQSVYVAT DSESYVPELQ QLFKGKVKVV SLKPEVAQVD LYILGQADHF IGNCVSSFTA 370 380 FVKRERDLQG RPSSFFGMDR PPKLRDEF

References

[1]

"A new superfamily of protein-O-fucosyltransferases, alpha2-fucosyltransferases, and alpha6-fucosyltransferases: phylogeny and identification of conserved peptide motifs." Martinez-Duncker I., Mollicone R., Candelier J.-J., Breton C., Oriol R. Glycobiology 13:1C-5C(2003) [PubMed: 12966037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ781500 mRNA. Translation: CAH03712.1.
RefSeq	NP_001008982.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT65. Glycosyltransferase Family 65.
Genome annotation databases
Ensembl	ENSPTRT00000024842; ENSPTRP00000022931; ENSPTRG00000013375; Pan troglodytes. [Genome view]
GeneID	449504.
KEGG	ptr:449504.
Organism-specific databases
CTD	449504.
Phylogenomic databases
HOVERGEN	Q6EV69.
OMA	QSVYIAT.
Enzyme and pathway databases
BRENDA	2.4.1.221. 264977.
Family and domain databases
InterPro	IPR019378. GDP-Fuc_prot_O-FucTrfase. [Graphical view]
Pfam	PF10250. O-FucT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OFUT1_PANTR

Accession

Primary (citable) accession number: Q6EV69

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)