Q6EV69 (OFUT1_PANTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GDP-fucose protein O-fucosyltransferase 1
EC=2.4.1.221

Alternative name(s):
Peptide-O-fucosyltransferase 1
Short name=O-FucT-1

Gene names

Name:	POFUT1
Synonyms:	FUT12

Organism

Pan troglodytes (Chimpanzee) [Reference proteome]

Taxonomic identifier

9598 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Pan

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs) By similarity.
Catalytic activity	Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum By similarity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the glycosyltransferase 68 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism Notch signaling pathway
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW angiogenesis Inferred from electronic annotation. Source: Compara fucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW heart development Inferred from electronic annotation. Source: Compara nervous system development Inferred from electronic annotation. Source: Compara protein O-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB somitogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	fucosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB peptide-O-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 388	362	GDP-fucose protein O-fucosyltransferase 1		PRO_0000012150
Regions
	Region	44 – 46	3	Substrate binding By similarity
	Region	238 – 240	3	Substrate binding By similarity
	Region	356 – 357	2	Substrate binding By similarity
	Motif	385 – 388	4	Prevents secretion from ER Potential
Sites
	Binding site	335	1	Substrate By similarity
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	160	1	N-linked (GlcNAc...) Potential
	Disulfide bond	38 ↔ 40		By similarity
	Disulfide bond	126 ↔ 140		By similarity
	Disulfide bond	249 ↔ 283		By similarity
	Disulfide bond	267 ↔ 354		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6EV69 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 3FACCCA434D02415	FASTA	388	43,956
	10 20 30 40 50 60 MGAAAWARPL SVSFLLLLLP LPGMPAGSWD PAGYLLYCPC MGRFGNQADH FLGSLAFAKL 70 80 90 100 110 120 LNRTLAVPPW IEYQHHKPPF TNLHVSYQKY FKLEPLQAYH RVISLEDFME KLAPTHWPPE 130 140 150 160 170 180 KRVAYCFEVA AQRSPDKKTC PMKEGNPFGP FWDQFHVSFN KSELFTGISF SASYREQWSQ 190 200 210 220 230 240 RFSPKEHPVL ALPGAPAQFP VLEEHRPLQK YMVWSDEMVK TGEAQIHAHL VRPYVGIHLR 250 260 270 280 290 300 IGSDWKNACA MLKDGTAGSH FMASPQCVGY SRSTAAPLTM TMCLPDLKEI QRAVKLWVRS 310 320 330 340 350 360 LDAQSVYVAT DSESYVPELQ QLFKGKVKVV SLKPEVAQVD LYILGQADHF IGNCVSSFTA 370 380 FVKRERDLQG RPSSFFGMDR PPKLRDEF

References

[1]

"A new superfamily of protein-O-fucosyltransferases, alpha2-fucosyltransferases, and alpha6-fucosyltransferases: phylogeny and identification of conserved peptide motifs." Martinez-Duncker I., Mollicone R., Candelier J.-J., Breton C., Oriol R. Glycobiology 13:1C-5C(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ781500 mRNA. Translation: CAH03712.1.
RefSeq	NP_001008982.1. NM_001008982.1.
UniGene	Ptr.6249.
3D structure databases
ProteinModelPortal	Q6EV69.
ModBase	Search...
Protein-protein interaction databases
STRING	9598.ENSPTRP00000022931.
Protein family/group databases
CAZy	GT65. Glycosyltransferase Family 65.
Proteomic databases
PRIDE	Q6EV69.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSPTRT00000024842; ENSPTRP00000022931; ENSPTRG00000013375.
GeneID	449504.
KEGG	ptr:449504.
Organism-specific databases
CTD	23509.
Phylogenomic databases
eggNOG	NOG250895.
GeneTree	ENSGT00390000015634.
HOGENOM	HOG000231651.
HOVERGEN	HBG059976.
InParanoid	Q6EV69.
KO	K03691.
OMA	RNGIDWV.
OrthoDB	EOG43TZVN.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR019378. GDP-Fuc_O-FucTrfase. [Graphical view]
Pfam	PF10250. O-FucT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20832632.

Entry information

Entry name

OFUT1_PANTR

Accession

Primary (citable) accession number: Q6EV69

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program