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Q6EV56

- OFUT2_PANTR

UniProt

Q6EV56 - OFUT2_PANTR

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Protein

GDP-fucose protein O-fucosyltransferase 2

Gene

POFUT2

Organism
Pan troglodytes (Chimpanzee)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13. O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity).By similarity

Catalytic activityi

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 541Proton donor/acceptorBy similarity
Binding sitei294 – 2941SubstrateBy similarity
Sitei396 – 3961Essential for catalytic activityBy similarity

GO - Molecular functioni

  1. peptide-O-fucosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. fucose metabolic process Source: UniProtKB-KW
  2. mesoderm formation Source: Ensembl
  3. protein O-linked fucosylation Source: UniProtKB
  4. regulation of epithelial to mesenchymal transition Source: Ensembl
  5. regulation of gene expression Source: Ensembl
  6. regulation of secretion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT68. Glycosyltransferase Family 68.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-fucose protein O-fucosyltransferase 2 (EC:2.4.1.221)
Alternative name(s):
Peptide-O-fucosyltransferase 2
Short name:
O-FucT-2
Gene namesi
Name:POFUT2
Synonyms:FUT13
OrganismiPan troglodytes (Chimpanzee)
Taxonomic identifieri9598 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan
ProteomesiUP000002277: Chromosome 21

Subcellular locationi

Endoplasmic reticulum By similarity. Golgi apparatus By similarity
Note: Mainly located in the endoplasmic reticulum.By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 429408GDP-fucose protein O-fucosyltransferase 2PRO_0000012156Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi161 ↔ 192By similarity
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi412 ↔ 419By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ6EV56.

Interactioni

Protein-protein interaction databases

STRINGi9598.ENSPTRP00000024102.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 564Substrate bindingBy similarity
Regioni388 – 3892Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 68 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG77810.
GeneTreeiENSGT00390000007989.
HOGENOMiHOG000015874.
HOVERGENiHBG053367.
InParanoidiQ6EV56.
KOiK03691.
OMAiICAHARF.
OrthoDBiEOG76T9S1.
TreeFamiTF314337.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6EV56-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATLSFVFLL LGAVSWPPAS ASGQEFWPGQ SAADILSGAA SRRRYLLYDV
60 70 80 90 100
NPPEGFNLRR DVYIRIASLL KTLLKTEEWV LVLPPWGRLY HWQSPDIHQV
110 120 130 140 150
RIPWSEFFDL PSLNKNIPVI EYEQFIAESG GPFIDQVYVL QSYAEGWKEG
160 170 180 190 200
TWEEKVDERP CIDQLLYSQD KHEYYRGWFW GYEETRGLNV SCLSVQGSAS
210 220 230 240 250
IVAPLLLRNT SARSVMLDRA ENLLHDHYGG KEYWDTRRSM VFARHLREVG
260 270 280 290 300
DEFRSRHLNS TDDADGIPFQ EDWTKMKVKL GSALGGPYLG VHLRRKDFIW
310 320 330 340 350
GHRQDVPSLE GAVRKIRSLM KTHRLDKVFV ATDAVRKEYE ELKKLLPEMV
360 370 380 390 400
RFEPTWEELE LYKDGGVAII DQWICAHARF FIGTSVSTFS FRIHEEREIL
410 420
GLDPKTTYNR FCGDQEKACE QPTHWKITY
Length:429
Mass (Da):49,847
Last modified:August 16, 2004 - v1
Checksum:i77A3635BD4698983
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ781756 mRNA. Translation: CAH03731.1.
RefSeqiNP_001008983.1. NM_001008983.1.
UniGeneiPtr.6191.

Genome annotation databases

EnsembliENSPTRT00000026129; ENSPTRP00000024102; ENSPTRG00000014001.
GeneIDi449505.
KEGGiptr:449505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ781756 mRNA. Translation: CAH03731.1 .
RefSeqi NP_001008983.1. NM_001008983.1.
UniGenei Ptr.6191.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9598.ENSPTRP00000024102.

Protein family/group databases

CAZyi GT68. Glycosyltransferase Family 68.

Proteomic databases

PRIDEi Q6EV56.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSPTRT00000026129 ; ENSPTRP00000024102 ; ENSPTRG00000014001 .
GeneIDi 449505.
KEGGi ptr:449505.

Organism-specific databases

CTDi 23275.

Phylogenomic databases

eggNOGi NOG77810.
GeneTreei ENSGT00390000007989.
HOGENOMi HOG000015874.
HOVERGENi HBG053367.
InParanoidi Q6EV56.
KOi K03691.
OMAi ICAHARF.
OrthoDBi EOG76T9S1.
TreeFami TF314337.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 20832633.

Family and domain databases

InterProi IPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view ]
Pfami PF10250. O-FucT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A new superfamily of protein-O-fucosyltransferases, alpha2-fucosyltransferases, and alpha6-fucosyltransferases: phylogeny and identification of conserved peptide motifs."
    Martinez-Duncker I., Mollicone R., Candelier J.-J., Breton C., Oriol R.
    Glycobiology 13:1C-5C(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiOFUT2_PANTR
AccessioniPrimary (citable) accession number: Q6EV56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: August 16, 2004
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3