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Q6EV56

- OFUT2_PANTR

UniProt

Q6EV56 - OFUT2_PANTR

Protein

GDP-fucose protein O-fucosyltransferase 2

Gene

POFUT2

Organism
Pan troglodytes (Chimpanzee)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13. O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm By similarity.By similarity

    Catalytic activityi

    Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei54 – 541Proton donor/acceptorBy similarity
    Binding sitei294 – 2941SubstrateBy similarity
    Sitei396 – 3961Essential for catalytic activityBy similarity

    GO - Molecular functioni

    1. peptide-O-fucosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. fucose metabolic process Source: UniProtKB-KW
    2. mesoderm formation Source: Ensembl
    3. protein O-linked fucosylation Source: UniProtKB
    4. regulation of epithelial to mesenchymal transition Source: Ensembl
    5. regulation of gene expression Source: Ensembl
    6. regulation of secretion Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Fucose metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT68. Glycosyltransferase Family 68.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GDP-fucose protein O-fucosyltransferase 2 (EC:2.4.1.221)
    Alternative name(s):
    Peptide-O-fucosyltransferase 2
    Short name:
    O-FucT-2
    Gene namesi
    Name:POFUT2
    Synonyms:FUT13
    OrganismiPan troglodytes (Chimpanzee)
    Taxonomic identifieri9598 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan
    ProteomesiUP000002277: Chromosome 21

    Subcellular locationi

    Endoplasmic reticulum By similarity. Golgi apparatus By similarity
    Note: Mainly located in the endoplasmic reticulum.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell
    2. Golgi apparatus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 429408GDP-fucose protein O-fucosyltransferase 2PRO_0000012156Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi161 ↔ 192By similarity
    Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi412 ↔ 419By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ6EV56.

    Interactioni

    Protein-protein interaction databases

    STRINGi9598.ENSPTRP00000024102.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni53 – 564Substrate bindingBy similarity
    Regioni388 – 3892Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 68 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG77810.
    GeneTreeiENSGT00390000007989.
    HOGENOMiHOG000015874.
    HOVERGENiHBG053367.
    InParanoidiQ6EV56.
    KOiK03691.
    OMAiICAHARF.
    OrthoDBiEOG76T9S1.
    TreeFamiTF314337.

    Family and domain databases

    InterProiIPR019378. GDP-Fuc_O-FucTrfase.
    [Graphical view]
    PfamiPF10250. O-FucT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6EV56-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLSFVFLL LGAVSWPPAS ASGQEFWPGQ SAADILSGAA SRRRYLLYDV    50
    NPPEGFNLRR DVYIRIASLL KTLLKTEEWV LVLPPWGRLY HWQSPDIHQV 100
    RIPWSEFFDL PSLNKNIPVI EYEQFIAESG GPFIDQVYVL QSYAEGWKEG 150
    TWEEKVDERP CIDQLLYSQD KHEYYRGWFW GYEETRGLNV SCLSVQGSAS 200
    IVAPLLLRNT SARSVMLDRA ENLLHDHYGG KEYWDTRRSM VFARHLREVG 250
    DEFRSRHLNS TDDADGIPFQ EDWTKMKVKL GSALGGPYLG VHLRRKDFIW 300
    GHRQDVPSLE GAVRKIRSLM KTHRLDKVFV ATDAVRKEYE ELKKLLPEMV 350
    RFEPTWEELE LYKDGGVAII DQWICAHARF FIGTSVSTFS FRIHEEREIL 400
    GLDPKTTYNR FCGDQEKACE QPTHWKITY 429
    Length:429
    Mass (Da):49,847
    Last modified:August 16, 2004 - v1
    Checksum:i77A3635BD4698983
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ781756 mRNA. Translation: CAH03731.1.
    RefSeqiNP_001008983.1. NM_001008983.1.
    UniGeneiPtr.6191.

    Genome annotation databases

    EnsembliENSPTRT00000026129; ENSPTRP00000024102; ENSPTRG00000014001.
    GeneIDi449505.
    KEGGiptr:449505.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ781756 mRNA. Translation: CAH03731.1 .
    RefSeqi NP_001008983.1. NM_001008983.1.
    UniGenei Ptr.6191.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9598.ENSPTRP00000024102.

    Protein family/group databases

    CAZyi GT68. Glycosyltransferase Family 68.

    Proteomic databases

    PRIDEi Q6EV56.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSPTRT00000026129 ; ENSPTRP00000024102 ; ENSPTRG00000014001 .
    GeneIDi 449505.
    KEGGi ptr:449505.

    Organism-specific databases

    CTDi 23275.

    Phylogenomic databases

    eggNOGi NOG77810.
    GeneTreei ENSGT00390000007989.
    HOGENOMi HOG000015874.
    HOVERGENi HBG053367.
    InParanoidi Q6EV56.
    KOi K03691.
    OMAi ICAHARF.
    OrthoDBi EOG76T9S1.
    TreeFami TF314337.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 20832633.

    Family and domain databases

    InterProi IPR019378. GDP-Fuc_O-FucTrfase.
    [Graphical view ]
    Pfami PF10250. O-FucT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new superfamily of protein-O-fucosyltransferases, alpha2-fucosyltransferases, and alpha6-fucosyltransferases: phylogeny and identification of conserved peptide motifs."
      Martinez-Duncker I., Mollicone R., Candelier J.-J., Breton C., Oriol R.
      Glycobiology 13:1C-5C(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiOFUT2_PANTR
    AccessioniPrimary (citable) accession number: Q6EV56
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3