Reviewed, UniProtKB/Swiss-Prot Q6ETN3 (4CL3_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable 4-coumarate--CoA ligase 3
      Short name=4CL 3
      Short name=Os4CL3
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 3

Gene names

Name:	4CL3
Ordered Locus Names:	Os02g0177600, LOC_Os02g08100
ORF Names:	P0504A05.24, OsJ_005431

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Probable 4-coumarate--CoA ligase 3		PRO_0000351624
Regions
	Nucleotide binding	188 – 196	9	ATP By similarity
	Nucleotide binding	331 – 336	6	ATP By similarity
	Region	261 – 330	70	SBD1
	Region	331 – 398	68	SBD2
Sites
	Binding site	419	1	ATP By similarity
	Binding site	434	1	ATP By similarity
	Binding site	525	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ETN3-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 5A1A9D84734BB13C	FASTA	554	59,641
	10 20 30 40 50 60 MGSVAAEEVV VFRSKLPDIE IDNSMTLQEY CFARMAEVGA RPCLIDGQTG ESYTYAEVES 70 80 90 100 110 120 ASRRAAAGLR RMGVGKGDVV MSLLRNCPEF AFSFLGAARL GAATTTANPF YTPHEVHRQA 130 140 150 160 170 180 EAAGARVIVT EACAVEKVRE FAAERGVPVV TVDGAFDGCV EFREVLAAEE LDADADVHPD 190 200 210 220 230 240 DVVALPYSSG TTGLPKGVML THRSLITSVA QQVDGENPNL YFSKDDVILC LLPLFHIYSL 250 260 270 280 290 300 NSVLLAGLRA GSTIVIMRKF DLGALVDLVR KHNITIAPFV PPIVVEIAKS PRVTAEDLAS 310 320 330 340 350 360 IRMVMSGAAP MGKDLQDAFM AKIPNAVLGQ GYGMTEAGPV LAMCLAFAKE PFKVKSGSCG 370 380 390 400 410 420 TVVRNAELKI VDPDTGTSLG RNQSGEICIR GEQIMKGYLN DPEATKNTID EDGWLHTGDI 430 440 450 460 470 480 GFVDDDDEIF IVDRLKEIIK YKGFQVPPAE LEALLITHPE IKDAAVVSMK DDLAGEVPVA 490 500 510 520 530 540 FIVRTEGSEI TEDEIKKFVA KEVVFYKRIN KVFFTDSIPK NPSGKILRKD LRARLAAGIP 550 DAVAAAAADA PKSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"4-coumarate:CoA ligase in Rice." Nakano Y., Satoh K., Mase K., Nishikubo N., Kitano H., Katayama Y. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Fujiminori.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella." de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J. New Phytol. 179:987-1003(2008) [PubMed: 18627494] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB234050 mRNA. Translation: BAF30962.1. AP004838 Genomic DNA. Translation: BAD27987.1. AP008208 Genomic DNA. Translation: BAF07983.1. CM000139 Genomic DNA. Translation: EAZ21948.1.
RefSeq	NP_001046069.1.
UniGene	Os.10669
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Genome annotation databases
GeneID	4328485.
GenomeReviews	Gene locus 4CL3 in contig AP008208_GR.
KEGG	osa:4328485.
Organism-specific databases
Gramene	Q6ETN3.
Phylogenomic databases
HOGENOM	HBG547964.
PhylomeDB	Q6ETN3.
Enzyme and pathway databases
BRENDA	6.2.1.12. 1975.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL3_ORYSJ

Accession

Primary (citable) accession number: Q6ETN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)