Reviewed, UniProtKB/Swiss-Prot Q6ER94 (BAS1_ORYSJ)

Names and origin

Protein names

Recommended name:
    2-Cys peroxiredoxin BAS1, chloroplastic
    EC=1.11.1.15
Alternative name(s):
    Thiol-specific antioxidant protein

Gene names

Name:	BAS1
Ordered Locus Names:	Os02g0537700, LOC_Os02g33450
ORF Names:	P0508B05.23, OSJNBa0014M17.2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf. Involved in the detoxification of alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.
Subunit structure	Homodimer; disulfide-linked, upon oxidation By similarity. Interacts with the plastidial NADPH-dependent thioredoxin reductase NTRC.
Subcellular location	Plastid › chloroplastBy similarity.
Post-translational modification	The Cys-114-SH group is the primary site of oxidation by H(2)O(2), and the oxidized Cys-114 (probably Cys-SOH) rapidly reacts with Cys-236-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced by thioredoxin By similarity.
Sequence similarities	Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 60	60	Chloroplast By similarity
	Chain	61 – 261	201	2-Cys peroxiredoxin BAS1, chloroplastic		PRO_0000284084
Regions
	Domain	68 – 227	160	Thioredoxin
Sites
	Active site	114	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	114		Interchain (with C-236); in linked form By similarity
	Disulfide bond	236		Interchain (with C-114); in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6ER94-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 5F0CA432FDA0007B	FASTA	261	28,097
	10 20 30 40 50 60 MAACCSSLAT AVSSSSAKPL AGIPPAAPHS LSLPRAPAAR PLRLSASSSR SARASSFVAR 70 80 90 100 110 120 AGGVDDAPLV GNKAPDFDAE AVFDQEFINV KLSDYIGKKY VILFFYPLDF TFVCPTEITA 130 140 150 160 170 180 FSDRYDEFEK LNTEILGVSI DSVFSHLAWV QTDRKSGGLG DLKYPLISDV TKSISKSFGV 190 200 210 220 230 240 LIPDQGIALR GLFIIDKEGV IQHSTINNLA IGRSVDETMR TLQALQYVQD NPDEVCPAGW 250 260 KPGDKSMKPD PKGSKEYFAA I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rice NTRC is a high-efficiency redox system for chloroplast protection against oxidative damage." Perez-Ruiz J.M., Spinola M.C., Kirchsteiger K., Moreno J., Sahrawy M., Cejudo F.J. Plant Cell 18:2356-2368(2006) [PubMed: 16891402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH NTRC. Strain: cv. Nipponbare. Tissue: Leaf.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed: 16758443] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 61-67, MASS SPECTROMETRY. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM039889 mRNA. Translation: CAJ01693.1. AP004753 Genomic DNA. Translation: BAD27915.1. AP005609 Genomic DNA. Translation: BAD28826.1. AP008208 Genomic DNA. Translation: BAF08964.1. AK104703 mRNA. No translation available.
RefSeq	NP_001047050.1.
UniGene	Os.12196
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	4022. Os2CysPrx.
Genome annotation databases
GeneID	4329578.
KEGG	osa:4329578.
Organism-specific databases
Gramene	Q6ER94.
Family and domain databases
InterPro	IPR000866. AhpC-TSA. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAS1_ORYSJ

Accession

Primary (citable) accession number: Q6ER94

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)