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Protein

2-Cys peroxiredoxin BAS1, chloroplastic

Gene

BAS1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:16891402, PubMed:19345687). May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf (By similarity).By similarity2 Publications

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

  • peroxidase activity Source: UniProtKB-KW
  • peroxiredoxin activity Source: UniProtKB

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • hydrogen peroxide catabolic process Source: UniProtKB

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase
Biological processHydrogen peroxide

Enzyme and pathway databases

ReactomeiR-OSA-3299685. Detoxification of Reactive Oxygen Species.
R-OSA-5628897. TP53 Regulates Metabolic Genes.
R-OSA-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei4022. Os2CysPrx.

Names & Taxonomyi

Protein namesi
Recommended name:
2-Cys peroxiredoxin BAS1, chloroplastic (EC:1.11.1.15)
Alternative name(s):
Thiol-specific antioxidant protein
Gene namesi
Name:BAS1
Ordered Locus Names:Os02g0537700, LOC_Os02g33450
ORF Names:OsJ_07037Imported, OSJNBa0014M17.2, P0508B05.23
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 60Chloroplast1 PublicationAdd BLAST60
ChainiPRO_000028408461 – 2612-Cys peroxiredoxin BAS1, chloroplasticAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi114Interchain (with C-236); in linked formBy similarity
Disulfide bondi236Interchain (with C-114); in linked formBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ6ER94.
PRIDEiQ6ER94.

Expressioni

Gene expression databases

GenevisibleiQ6ER94. OS.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation (By similarity). Interacts with the plastidial NADPH-dependent thioredoxin reductase NTRC (PubMed:19345687).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Os07g0657900Q70G585EBI-6956385,EBI-6956411

Protein-protein interaction databases

IntActiQ6ER94. 2 interactors.
MINTiMINT-7017080.
STRINGi39947.LOC_Os02g33450.1.

Structurei

3D structure databases

ProteinModelPortaliQ6ER94.
SMRiQ6ER94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 227ThioredoxinPROSITE-ProRule annotationAdd BLAST160

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
InParanoidiQ6ER94.
KOiK03386.
OMAiCPANWEE.
OrthoDBiEOG09360MKG.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6ER94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAACCSSLAT AVSSSSAKPL AGIPPAAPHS LSLPRAPAAR PLRLSASSSR
60 70 80 90 100
SARASSFVAR AGGVDDAPLV GNKAPDFDAE AVFDQEFINV KLSDYIGKKY
110 120 130 140 150
VILFFYPLDF TFVCPTEITA FSDRYDEFEK LNTEILGVSI DSVFSHLAWV
160 170 180 190 200
QTDRKSGGLG DLKYPLISDV TKSISKSFGV LIPDQGIALR GLFIIDKEGV
210 220 230 240 250
IQHSTINNLA IGRSVDETMR TLQALQYVQD NPDEVCPAGW KPGDKSMKPD
260
PKGSKEYFAA I
Length:261
Mass (Da):28,097
Last modified:August 16, 2004 - v1
Checksum:i5F0CA432FDA0007B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039889 mRNA. Translation: CAJ01693.1.
AP004753 Genomic DNA. Translation: BAD27915.1.
AP005609 Genomic DNA. Translation: BAD28826.1.
AP008208 Genomic DNA. Translation: BAF08964.1.
AP014958 Genomic DNA. Translation: BAS79077.1.
CM000139 Genomic DNA. Translation: EEE57135.1.
AK068919 mRNA. Translation: BAG91160.1.
AK104703 mRNA. Translation: BAG96892.1.
RefSeqiXP_015627405.1. XM_015771919.1.
UniGeneiOs.12196.

Genome annotation databases

EnsemblPlantsiOS02T0537700-01; OS02T0537700-01; OS02G0537700.
GeneIDi4329578.
GrameneiOS02T0537700-01; OS02T0537700-01; OS02G0537700.
KEGGiosa:4329578.

Similar proteinsi

Entry informationi

Entry nameiBAS1_ORYSJ
AccessioniPrimary (citable) accession number: Q6ER94
Secondary accession number(s): B7EFL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: August 16, 2004
Last modified: August 30, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families