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Protein

2-Cys peroxiredoxin BAS1, chloroplastic

Gene

BAS1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antioxidant enzyme that reduces hydrogen peroxide in chloroplasts. Participates in a NADPH-dependent hydrogen peroxide scavenging system in chloroplasts by receiving reducing equivalents from the thioredoxin reductase NTRC. May be involved in the detoxification of alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system.2 Publications

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141Cysteine sulfenic acid (-SOH) intermediateBy similarity

GO - Molecular functioni

  • peroxidase activity Source: UniProtKB-KW
  • peroxiredoxin activity Source: UniProtKB

GO - Biological processi

  • hydrogen peroxide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Enzyme and pathway databases

ReactomeiR-OSA-3299685. Detoxification of Reactive Oxygen Species.
R-OSA-5628897. TP53 Regulates Metabolic Genes.

Protein family/group databases

PeroxiBasei4022. Os2CysPrx.

Names & Taxonomyi

Protein namesi
Recommended name:
2-Cys peroxiredoxin BAS1, chloroplastic (EC:1.11.1.15)
Alternative name(s):
Thiol-specific antioxidant protein
Gene namesi
Name:BAS1
Ordered Locus Names:Os02g0537700, LOC_Os02g33450
ORF Names:OsJ_07037Imported, OSJNBa0014M17.2, P0508B05.23
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi114 – 1141C → S: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6060Chloroplast1 PublicationAdd
BLAST
Chaini61 – 2612012-Cys peroxiredoxin BAS1, chloroplasticPRO_0000284084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi114 – 114Interchain (with C-236); in linked formBy similarity
Disulfide bondi236 – 236Interchain (with C-114); in linked formBy similarity

Post-translational modificationi

The Cys-114-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-114 (probably Cys-SOH) rapidly reacts with Cys-236-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced by thioredoxin (By similarity).By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ6ER94.
PRIDEiQ6ER94.

Expressioni

Gene expression databases

ExpressionAtlasiQ6ER94. baseline and differential.
GenevisibleiQ6ER94. OS.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. Interacts with the thioredoxin reductase NTRC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Os07g0657900Q70G585EBI-6956385,EBI-6956411

Protein-protein interaction databases

IntActiQ6ER94. 2 interactions.
MINTiMINT-7017080.
STRINGi39947.LOC_Os02g33450.1.

Structurei

3D structure databases

ProteinModelPortaliQ6ER94.
SMRiQ6ER94. Positions 70-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 227160ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AhpC/TSA family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
InParanoidiQ6ER94.
OMAiVQPFKTE.
OrthoDBiEOG09360MKG.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6ER94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAACCSSLAT AVSSSSAKPL AGIPPAAPHS LSLPRAPAAR PLRLSASSSR
60 70 80 90 100
SARASSFVAR AGGVDDAPLV GNKAPDFDAE AVFDQEFINV KLSDYIGKKY
110 120 130 140 150
VILFFYPLDF TFVCPTEITA FSDRYDEFEK LNTEILGVSI DSVFSHLAWV
160 170 180 190 200
QTDRKSGGLG DLKYPLISDV TKSISKSFGV LIPDQGIALR GLFIIDKEGV
210 220 230 240 250
IQHSTINNLA IGRSVDETMR TLQALQYVQD NPDEVCPAGW KPGDKSMKPD
260
PKGSKEYFAA I
Length:261
Mass (Da):28,097
Last modified:August 16, 2004 - v1
Checksum:i5F0CA432FDA0007B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039889 mRNA. Translation: CAJ01693.1.
AP004753 Genomic DNA. Translation: BAD27915.1.
AP005609 Genomic DNA. Translation: BAD28826.1.
AP008208 Genomic DNA. Translation: BAF08964.1.
AP014958 Genomic DNA. Translation: BAS79077.1.
CM000139 Genomic DNA. Translation: EEE57135.1.
AK068919 mRNA. Translation: BAG91160.1.
AK104703 mRNA. Translation: BAG96892.1.
RefSeqiXP_015627405.1. XM_015771919.1.
UniGeneiOs.12196.

Genome annotation databases

EnsemblPlantsiOS02T0537700-01; OS02T0537700-01; OS02G0537700.
GeneIDi4329578.
GrameneiOS02T0537700-01; OS02T0537700-01; OS02G0537700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039889 mRNA. Translation: CAJ01693.1.
AP004753 Genomic DNA. Translation: BAD27915.1.
AP005609 Genomic DNA. Translation: BAD28826.1.
AP008208 Genomic DNA. Translation: BAF08964.1.
AP014958 Genomic DNA. Translation: BAS79077.1.
CM000139 Genomic DNA. Translation: EEE57135.1.
AK068919 mRNA. Translation: BAG91160.1.
AK104703 mRNA. Translation: BAG96892.1.
RefSeqiXP_015627405.1. XM_015771919.1.
UniGeneiOs.12196.

3D structure databases

ProteinModelPortaliQ6ER94.
SMRiQ6ER94. Positions 70-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6ER94. 2 interactions.
MINTiMINT-7017080.
STRINGi39947.LOC_Os02g33450.1.

Protein family/group databases

PeroxiBasei4022. Os2CysPrx.

Proteomic databases

PaxDbiQ6ER94.
PRIDEiQ6ER94.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS02T0537700-01; OS02T0537700-01; OS02G0537700.
GeneIDi4329578.
GrameneiOS02T0537700-01; OS02T0537700-01; OS02G0537700.

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
InParanoidiQ6ER94.
OMAiVQPFKTE.
OrthoDBiEOG09360MKG.

Enzyme and pathway databases

ReactomeiR-OSA-3299685. Detoxification of Reactive Oxygen Species.
R-OSA-5628897. TP53 Regulates Metabolic Genes.

Gene expression databases

ExpressionAtlasiQ6ER94. baseline and differential.
GenevisibleiQ6ER94. OS.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000866. AhpC/TSA.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAS1_ORYSJ
AccessioniPrimary (citable) accession number: Q6ER94
Secondary accession number(s): B7EFL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.