ASMT1

Functionⁱ

Methyltransferase which catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).4 Publications

Catalytic activityⁱ

S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin.1 Publication

Kineticsⁱ

K_M=
222 µM for N-acetylserotonin (at 55 degrees Celsius)
1 Publication
Manual assertion based on experiment inⁱ
- Ref.11
  "Cellular localization and kinetics of the rice melatonin biosynthetic enzymes SNAT and ASMT."
  Byeon Y., Lee H.Y., Lee K., Park S., Back K.
  J. Pineal Res. 56:107-114(2014) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
K_M=
864 µM for N-acetylserotonin (at 30 degrees Celsius)
1 Publication
Manual assertion based on experiment inⁱ
- Ref.8
  "Kinetic analysis of purified recombinant rice N-acetylserotonin methyltransferase and peak melatonin production in etiolated rice shoots."
  Park S., Byeon Y., Kim Y.S., Back K.
  J. Pineal Res. 54:139-144(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION BY DARK.

pH dependenceⁱ

Optimum pH is 7.8.1 Publication

Temperature dependenceⁱ

Optimum temperature is 55 degrees Celsius.1 Publication

Pathwayⁱ: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.Curated
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Serotonin N-acetyltransferase 1, chloroplastic (SNAT1), Acetylserotonin O-methyltransferase 2 (ASMT2), Serotonin N-acetyltransferase 2, chloroplastic (SNAT2), Acetylserotonin O-methyltransferase 1 (ASMT1), Acetylserotonin O-methyltransferase 3 (ASMT3)
no protein annotated in this organism

This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	208	S-adenosyl-L-methionine; via carbonyl oxygenBy similarity	1
Binding siteⁱ	231	S-adenosyl-L-methioninePROSITE-ProRule annotation	1
Binding siteⁱ	251	S-adenosyl-L-methionineBy similarity	1
Binding siteⁱ	252	S-adenosyl-L-methionine; via amide nitrogenBy similarity	1
Binding siteⁱ	265	S-adenosyl-L-methionineBy similarity	1
Active siteⁱ	269	Proton acceptorPROSITE-ProRule annotation	1
Active siteⁱ	300	By similarity	1
Active siteⁱ	330	By similarity	1

GO - Molecular functionⁱ

acetylserotonin O-methyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 24134674
protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

melatonin biosynthetic process
Source: UniProtKB
Inferred from direct assayⁱ
- 24134674

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	Melatonin biosynthesis
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

Reactomeⁱ	R-OSA-209931. Serotonin and melatonin biosynthesis.
SABIO-RKⁱ	Q6EPG8.
UniPathwayⁱ	UPA00837; UER00815.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Acetylserotonin O-methyltransferase 1Curated (EC:2.1.1.41 Publication) Short name: OsASMT11 Publication
Gene namesⁱ	Name:ASMT11 Publication Synonyms:ASMT1 Publication Ordered Locus Names:Os09g0344500Imported, LOC_Os09g17560Curated ORF Names:OSJNBb0057I13.39Imported, OSJNBb0085I16.5Imported
Organismⁱ	Oryza sativa subsp. japonica (Rice)
Taxonomic identifierⁱ	39947 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › Liliopsida › Petrosaviidae › commelinids › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa
Proteomesⁱ	UP000059680 Componentⁱ: Chromosome 9

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000437947	1 – 364	Acetylserotonin O-methyltransferase 1Add BLAST		364

Proteomic databases

PaxDbⁱ	Q6EPG8.

PaxDbⁱ

Q6EPG8.

Expressionⁱ

Tissue specificityⁱ

Expressed in leaves, stems and flowers.1 Publication

Inductionⁱ

By senescence, abscisic acid (ABA), acifluorfen, salt and cadmium (PubMed:21210840). Induced by copper (PubMed:18676621, PubMed:21210840). Induced by butafenacil (PubMed:22998587). Induced in dark-grown etiolated shoot (PubMed:22747959). Induced by UV-C (PubMed:24035516).5 Publications

Gene expression databases

ExpressionAtlasⁱ	Q6EPG8. baseline and differential.

ExpressionAtlasⁱ

Q6EPG8. baseline and differential.

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os09g17560.1.

STRINGⁱ

39947.LOC_Os09g17560.1.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q6EPG8.
SMRⁱ	Q6EPG8.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3178. Eukaryota. ENOG410XS7T. LUCA.
HOGENOMⁱ	HOG000238277.
OMAⁱ	NEQVMST.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360DNU.

Family and domain databases

Gene3Dⁱ	1.10.10.10. 1 hit.
InterProⁱ	View protein in InterPro IPR016461. O-MeTrfase_COMT. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR029063. SAM-dependent_MTases. IPR036388. WH-like_DNA-bd_sf. IPR036390. WH_DNA-bd_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit.
PIRSFⁱ	PIRSF005739. O-mtase. 1 hit.
SUPFAMⁱ	SSF46785. SSF46785. 1 hit. SSF53335. SSF53335. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51683. SAM_OMT_II. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q6EPG8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAQNVQENEQ VMSTEDLLQA QIELYHHCLA FIKSMALRAA TDLRIPDAIH 
        60         70         80         90        100
CNGGAATLTD LAAHVGLHPT KLSHLRRLMR VLTLSGIFTV HDGDGEATYT 
       110        120        130        140        150
LTRVSRLLLS DGVERTHGLS QMVRVFVNPV AVASQFSLHE WFTVEKAAAV 
       160        170        180        190        200
SLFEVAHGCT RWEMIANDSK DGSMFNAGMV EDSSVAMDII LRKSSNVFRG 
       210        220        230        240        250
INSLVDVGGG YGAVAAAVVR AFPDIKCTVL DLPHIVAKAP SNNNIQFVGG 
       260        270        280        290        300
DLFEFIPAAD VVLLKCILHC WQHDDCVKIM RRCKEAISAR DAGGKVILIE 
       310        320        330        340        350
VVVGIGSNET VPKEMQLLFD VFMMYTDGIE REEHEWKKIF LEAGFSDYKI 
       360 
IPVLGVRSII EVYP

Length:364

Mass (Da):40,095

Last modified:August 16, 2004 - v1

Checksum:ⁱB77C00F1EC496AB1

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP005700 Genomic DNA. Translation: BAD29092.1. AP005881 Genomic DNA. Translation: BAD29452.1. AP008215 Genomic DNA. Translation: BAF24845.1. AP014965 Genomic DNA. Translation: BAT07597.1. AK072740 mRNA. Translation: BAG93122.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_015610997.1. XM_015755511.1. XP_015610998.1. XM_015755512.1.
UniGeneⁱ	Os.53458.

Genome annotation databases

EnsemblPlantsⁱ	Os09t0344500-01; Os09t0344500-01; Os09g0344500.
GeneIDⁱ	4346795.
Grameneⁱ	Os09t0344500-01; Os09t0344500-01; Os09g0344500.
KEGGⁱ	osa:4346795.

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q6EPG8	A0A0E0QQ29 A0A0E0M035 A0A0E0EQZ7	Oryza rufipogon (Brownbeard rice) (Asian wild rice) Oryza punctata (Red rice) Oryza meridionalis	364	UniRef90_Q6EPG8	Cluster: Acetylserotonin O-methyltransferase 1	4

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q6EPG8	A0A0E0QQ29 A0A0E0M035 A0A0E0EQZ7 Q8VWG4 Q8VWG4-2 A0A0E0QVR2 I1QS93 A0A0D3HAN1 A0A0E0B794 A2Z4K1 +41	Oryza rufipogon (Brownbeard rice) (Asian wild rice) Oryza punctata (Red rice) Oryza meridionalis Oryza sativa subsp. japonica (Rice) Oryza glaberrima (African rice) Oryza barthii Oryza glumipatula Oryza sativa subsp. indica (Rice) Triticum aestivum (Wheat) Aegilops tauschii (Tausch's goatgrass) (Aegilops squarrosa) And more	364	UniRef50_Q6EPG8	Cluster: Acetylserotonin O-methyltransferase 1	52

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP005700 Genomic DNA. Translation: BAD29092.1. AP005881 Genomic DNA. Translation: BAD29452.1. AP008215 Genomic DNA. Translation: BAF24845.1. AP014965 Genomic DNA. Translation: BAT07597.1. AK072740 mRNA. Translation: BAG93122.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_015610997.1. XM_015755511.1. XP_015610998.1. XM_015755512.1.
UniGeneⁱ	Os.53458.

3D structure databases

ProteinModelPortalⁱ	Q6EPG8.
SMRⁱ	Q6EPG8.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os09g17560.1.

STRINGⁱ

39947.LOC_Os09g17560.1.

Proteomic databases

PaxDbⁱ	Q6EPG8.

PaxDbⁱ

Q6EPG8.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	Os09t0344500-01; Os09t0344500-01; Os09g0344500.
GeneIDⁱ	4346795.
Grameneⁱ	Os09t0344500-01; Os09t0344500-01; Os09g0344500.
KEGGⁱ	osa:4346795.

Phylogenomic databases

eggNOGⁱ	KOG3178. Eukaryota. ENOG410XS7T. LUCA.
HOGENOMⁱ	HOG000238277.
OMAⁱ	NEQVMST.
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360DNU.

Enzyme and pathway databases

UniPathwayⁱ	UPA00837; UER00815.
Reactomeⁱ	R-OSA-209931. Serotonin and melatonin biosynthesis.
SABIO-RKⁱ	Q6EPG8.

Gene expression databases

ExpressionAtlasⁱ	Q6EPG8. baseline and differential.

ExpressionAtlasⁱ

Q6EPG8. baseline and differential.

Family and domain databases

Gene3Dⁱ	1.10.10.10. 1 hit.
InterProⁱ	View protein in InterPro IPR016461. O-MeTrfase_COMT. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR029063. SAM-dependent_MTases. IPR036388. WH-like_DNA-bd_sf. IPR036390. WH_DNA-bd_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit.
PIRSFⁱ	PIRSF005739. O-mtase. 1 hit.
SUPFAMⁱ	SSF46785. SSF46785. 1 hit. SSF53335. SSF53335. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51683. SAM_OMT_II. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ASMT1_ORYSJ
Accessionⁱ	Q6EPG8Primary (citable) accession number: Q6EPG8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 2, 2016
	Last sequence update:	August 16, 2004
	Last modified:	February 28, 2018
	This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q6EPG8 (ASMT1_ORYSJ)

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Acetylserotonin O-methyltransferase 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: melatonin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Kineticsⁱ

pH dependenceⁱ

Pathwayⁱ: melatonin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ