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Protein

Acetylserotonin O-methyltransferase 1

Gene

ASMT1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).4 Publications

Catalytic activityi

S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin.1 Publication

Kineticsi

  1. KM=222 µM for N-acetylserotonin (at 55 degrees Celsius)1 Publication
  2. KM=864 µM for N-acetylserotonin (at 30 degrees Celsius)1 Publication
  1. Vmax=9 nmol/min/mg enzyme with N-acetylserotonin as substrate at 55 degrees Celsius)1 Publication
  2. Vmax=13.8 pmol/min/mg enzyme with N-acetylserotonin as substrate (at 30 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.8.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius.1 Publication

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.Curated
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serotonin N-acetyltransferase 1, chloroplastic (SNAT1), Acetylserotonin O-methyltransferase 2 (ASMT2), Serotonin N-acetyltransferase 2, chloroplastic (SNAT2), Acetylserotonin O-methyltransferase 3 (ASMT3), Acetylserotonin O-methyltransferase 1 (ASMT1)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei231S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei251S-adenosyl-L-methionineBy similarity1
Binding sitei252S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei265S-adenosyl-L-methionineBy similarity1
Active sitei269Proton acceptorPROSITE-ProRule annotation1
Active sitei300By similarity1
Active sitei330By similarity1

GO - Molecular functioni

  • acetylserotonin O-methyltransferase activity Source: UniProtKB
  • protein dimerization activity Source: InterPro

GO - Biological processi

  • melatonin biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processMelatonin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-OSA-209931. Serotonin and melatonin biosynthesis.
SABIO-RKiQ6EPG8.
UniPathwayiUPA00837; UER00815.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylserotonin O-methyltransferase 1Curated (EC:2.1.1.41 Publication)
Short name:
OsASMT11 Publication
Gene namesi
Name:ASMT11 Publication
Synonyms:ASMT1 Publication
Ordered Locus Names:Os09g0344500Imported, LOC_Os09g17560Curated
ORF Names:OSJNBb0057I13.39Imported, OSJNBb0085I16.5Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 9

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004379471 – 364Acetylserotonin O-methyltransferase 1Add BLAST364

Proteomic databases

PaxDbiQ6EPG8.

Expressioni

Tissue specificityi

Expressed in leaves, stems and flowers.1 Publication

Inductioni

By senescence, abscisic acid (ABA), acifluorfen, salt and cadmium (PubMed:21210840). Induced by copper (PubMed:18676621, PubMed:21210840). Induced by butafenacil (PubMed:22998587). Induced in dark-grown etiolated shoot (PubMed:22747959). Induced by UV-C (PubMed:24035516).5 Publications

Gene expression databases

ExpressionAtlasiQ6EPG8. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi39947.LOC_Os09g17560.1.

Structurei

3D structure databases

ProteinModelPortaliQ6EPG8.
SMRiQ6EPG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
HOGENOMiHOG000238277.
OMAiNEQVMST.
OrthoDBiEOG09360DNU.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR012967. Plant_MeTrfase_dimerisation.
IPR029063. SAM-dependent_MTases.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PfamiView protein in Pfam
PF08100. Dimerisation. 1 hit.
PF00891. Methyltransf_2. 1 hit.
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51683. SAM_OMT_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6EPG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNVQENEQ VMSTEDLLQA QIELYHHCLA FIKSMALRAA TDLRIPDAIH
60 70 80 90 100
CNGGAATLTD LAAHVGLHPT KLSHLRRLMR VLTLSGIFTV HDGDGEATYT
110 120 130 140 150
LTRVSRLLLS DGVERTHGLS QMVRVFVNPV AVASQFSLHE WFTVEKAAAV
160 170 180 190 200
SLFEVAHGCT RWEMIANDSK DGSMFNAGMV EDSSVAMDII LRKSSNVFRG
210 220 230 240 250
INSLVDVGGG YGAVAAAVVR AFPDIKCTVL DLPHIVAKAP SNNNIQFVGG
260 270 280 290 300
DLFEFIPAAD VVLLKCILHC WQHDDCVKIM RRCKEAISAR DAGGKVILIE
310 320 330 340 350
VVVGIGSNET VPKEMQLLFD VFMMYTDGIE REEHEWKKIF LEAGFSDYKI
360
IPVLGVRSII EVYP
Length:364
Mass (Da):40,095
Last modified:August 16, 2004 - v1
Checksum:iB77C00F1EC496AB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP005700 Genomic DNA. Translation: BAD29092.1.
AP005881 Genomic DNA. Translation: BAD29452.1.
AP008215 Genomic DNA. Translation: BAF24845.1.
AP014965 Genomic DNA. Translation: BAT07597.1.
AK072740 mRNA. Translation: BAG93122.1.
RefSeqiXP_015610997.1. XM_015755511.1.
XP_015610998.1. XM_015755512.1.
UniGeneiOs.53458.

Genome annotation databases

EnsemblPlantsiOS09T0344500-01; OS09T0344500-01; OS09G0344500.
GeneIDi4346795.
GrameneiOS09T0344500-01; OS09T0344500-01; OS09G0344500.
KEGGiosa:4346795.

Similar proteinsi

Entry informationi

Entry nameiASMT1_ORYSJ
AccessioniPrimary (citable) accession number: Q6EPG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2016
Last sequence update: August 16, 2004
Last modified: December 20, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families